bims-redobi 2024-08-18 papers

Issue of 2024‒08‒18
three papers selected by
Vanesa Cepas López, Candiolo Cancer Institute

Clin Chim Acta. 2024 Aug 10. pii: S0009-8981(24)02168-5. [Epub ahead of print]563 119915

Unveiling thiol biomarkers: Glutathione and cysteamine.

M G Gopika, Surya Gopidas, Gokul S Jayan, P S Arathy, Beena Saraswathyamma.

The physiological and clinical importance of Glutathione and Cysteamine is emphasized by their participation in a range of conditions, such as diabetes, cancer, renal failure, Parkinson's disease, and hypothyroidism. This necessitates the requirement for accessible, expedited, and cost-efficient testing that can facilitate clinical diagnosis and treatment options. This article examines numerous techniques used to detect both glutathione and cysteamine. The discussed methods include electroanalytical techniques such as voltammetry and amperometry, which are examined for their sensitivity and ability to provide real-time analysis. Furthermore, this study investigates the accuracy of gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) in measuring the concentrations of glutathione and cysteamine. Additionally, the potential of new nanotechnology-based methods, such as plasmonic nanoparticles and quantum dots, to improve the sensitivity of detecting glutathione and cysteamine is emphasized.

Keywords: Biosensors; Cysteamine; Electrochemical sensors; Glutathione; Portable sensors

DOI: https://doi.org/10.1016/j.cca.2024.119915

J Fluoresc. 2024 Aug 13.

A Versatile pH-Dependent Fluorometric, Colorimetric, and Electrochemical Sensor for H2S Monitoring in Water.

Rashmi M Kulkarni, M Ranjana, Namratha Ullal, Dhanya Sunil.

Hydrogen sulfide (H2S) is a colorless, foul smelling, toxic substance that can be found in water bodies and waste waters, especially in occupational susceptible environments, and can lead to harmful effects in humans at higher concentrations. An H2S monitoring probe NNAP is synthesized, which displays pH-dependent electrochemical, colorimetric, and fluorescence responses. NNAP functions as a fluorometric sensor at pH 7.4, with a limit of detection (LOD) of 0.70 mM, and as a colorimetric sensor at pH 12, where visible color changes are discernible to the naked eye, with an LOD of 4.28 mM. Additionally, it demonstrates utility in electrochemical sensing at pH 2, with a LOD of 2.5 mM. Furthermore, NNAP-coated paper strips have been successfully utilized for real-time H2S monitoring applications.

Keywords: Colorimetry; Electrochemical; Fluorometry; pH dependant H2S monitoring

DOI: https://doi.org/10.1007/s10895-024-03902-7

Molecules. 2024 Aug 03. pii: 3687. [Epub ahead of print]29(15):

Dynamic and Static Regulation of Nicotinamide Adenine Dinucleotide Phosphate: Strategies, Challenges, and Future Directions in Metabolic Engineering.

Nana Ding, Zenan Yuan, Lei Sun, Lianghong Yin.

Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is a crucial cofactor in metabolic networks. The efficient regeneration of NADPH is one of the limiting factors for productivity in biotransformation processes. To date, many metabolic engineering tools and static regulation strategies have been developed to regulate NADPH regeneration. However, traditional static regulation methods often lead to the NADPH/NADP+ imbalance, causing disruptions in cell growth and production. These methods also fail to provide real-time monitoring of intracellular NADP(H) or NADPH/NADP+ levels. In recent years, various biosensors have been developed for the detection, monitoring, and dynamic regulate of the intracellular NADP(H) levels or the NADPH/NADP+ balance. These NADPH-related biosensors are mainly used in the cofactor engineering of bacteria, yeast, and mammalian cells. This review analyzes and summarizes the NADPH metabolic regulation strategies from both static and dynamic perspectives, highlighting current challenges and potential solutions, and discusses future directions for the advanced regulation of the NADPH/NADP+ balance.

Keywords: NADP(H); dynamic regulation; metabolic engineering; redox balance; static regulation

DOI: https://doi.org/10.3390/molecules29153687