bims-polyam 2022-08-14 papers

Issue of 2022‒08‒14
four papers selected by
Sebastian J. Hofer
University of Graz

Int J Mol Sci. 2022 Jul 29. pii: 8394. [Epub ahead of print]23(15):

Light Spectral Composition Modifies Polyamine Metabolism in Young Wheat Plants.

Magda Pál, Kamirán Áron Hamow, Altafur Rahman, Imre Majláth, Judit Tajti, Orsolya Kinga Gondor, Mohamed Ahres, Fatemeh Gholizadeh, Gabriella Szalai, Tibor Janda.

Although light-emitting diode (LED) technology has extended the research on targeted photomorphogenic, physiological, and biochemical responses in plants, there is not enough direct information about how light affects polyamine metabolism. In this study, the effect of three spectral compositions (referred to by their most typical characteristic: blue, red, and the combination of blue and red [pink] lights) on polyamine metabolism was compared to those obtained under white light conditions at the same light intensity. Although light quality induced pronounced differences in plant morphology, pigment contents, and the expression of polyamine metabolism-related genes, endogenous polyamine levels did not differ substantially. When exogenous polyamines were applied, their roborative effect were detected under all light conditions, but these beneficial changes were correlated with an increase in polyamine content and polyamine metabolism-related gene expression only under blue light. The effect of the polyamines on leaf gene expression under red light was the opposite, with a decreasing tendency. Results suggest that light quality may optimize plant growth through the adjustment of polyamine metabolism at the gene expression level. Polyamine treatments induced different strategies in fine-tuning of polyamine metabolism, which were induced for optimal plant growth and development under different spectral compositions.

Keywords: blue light; light quality; putrescine; red light; spermidine; spermine; wheat

DOI: https://doi.org/10.3390/ijms23158394

Plant Physiol Biochem. 2022 Jul 19. pii: S0981-9428(22)00316-3. [Epub ahead of print]187 1-10

Exogenous spermidine modulates polyamine metabolism and improves stress responsive mechanisms to protect tomato seedlings against salt stress.

Abdul Raziq, Atta Mohi Ud Din, Sumera Anwar, Yu Wang, Mohammad Shah Jahan, Mingming He, Chen Guang Ling, Jin Sun, Sheng Shu, Shirong Guo.

Salt stress negatively affects plant growth, development, and crop productivity causing serious economic loss to agricultural production. Here, we investigated the exogenous application of spermidine (Spd) on tomato seedlings grown under salt stress. Salt stress reduced plant growth, biomass accumulation and chlorophyll contents, thus negatively affecting photosynthesis. Alternatively, Spd application effectively reduced the salinity-induced adverse effects in tomato seedlings by activating the H2O2 mediated signaling involving the enhanced expression of RBOH1 and salt stress-responsive genes SlMYB102, SlHKT1, SlWRKY1 and SlDREB2, and improving detoxification through higher antioxidative activity and osmolyte (proline) accumulation under salt stress. It was further confirmed by significantly lower amount of H2O2, malondialdehyde and electrolyte leakage, and better ion homeostasis (Na+/K+ ratio) and photosynthetic performance of Spd-treated seedlings under salt stress. Furthermore, Spd application modulated endogenous polyamines and enhanced the biosynthesis of endogenous Spd and spermine from putrescine. Altogether, these results confirm the important role of Spd against salt stress and suggest that the increased endogenous Spd content in plants could regulate a number of stress-responsive mechanisms to protect tomato seedlings against salt stress. These results provide a good direction for further elucidation of the detailed interplay between polyamine metabolism and H2O2-mediated signaling, which would help to improve abiotic stress tolerance in plants.

Keywords: H(2)O(2); Ion homeostasis; Polyamine metabolism; Salt stress; Spermidine; Tomato

DOI: https://doi.org/10.1016/j.plaphy.2022.07.005

Int J Mol Sci. 2022 Aug 02. pii: 8563. [Epub ahead of print]23(15):

Utilization of Aminoguanidine Prevents Cytotoxic Effects of Semen.

Mirja Harms, Pascal von Maltitz, Rüdiger Groß, Benjamin Mayer, Miriam Deniz, Janis Müller, Jan Münch.

Studies of human semen in cell or tissue culture are hampered by the high cytotoxic activity of this body fluid. The components responsible for the cell damaging activity of semen are amine oxidases, which convert abundant polyamines, such as spermine or spermidine in seminal plasma into toxic intermediates. Amine oxidases are naturally present at low concentrations in seminal plasma and at high concentrations in fetal calf serum, a commonly used cell culture supplement. Here, we show that, in the presence of fetal calf serum, seminal plasma, as well as the polyamines spermine and spermidine, are highly cytotoxic to immortalized cells, primary blood mononuclear cells, and vaginal tissue. Thus, experiments investigating the effect of polyamines and seminal plasma on cellular functions should be performed with great caution, considering the confounding cytotoxic effects. The addition of the amine oxidase inhibitor aminoguanidine to fetal calf serum and/or the utilization of serum-free medium greatly reduced this serum-induced cytotoxicity of polyamines and seminal plasma in cell lines, primary cells, and tissues and, thus, should be implemented in all future studies analyzing the role of polyamines and semen on cellular functions.

Keywords: cytotoxicity; polyamines; seminal fluid; spermine

DOI: https://doi.org/10.3390/ijms23158563

J Chem Inf Model. 2022 Aug 10.

Bovine Serum Amine Oxidase and Polyamine Analogues: Chemical Synthesis and Biological Evaluation Integrated with Molecular Docking and 3-D QSAR Studies.

Rino Ragno, Anna Minarini, Eleonora Proia, Antonini Lorenzo, Andrea Milelli, Vincenzo Tumiatti, Marco Fiore, Pasquale Fino, Lavinia Rutigliano, Rossella Fioravanti, Tomoaki Tahara, Elena Pacella, Antonio Greco, Gianluca Canettieri, Maria Luisa Di Paolo, Enzo Agostinelli.

Natural polyamines (PAs) are key players in cellular homeostasis by regulating cell growth and proliferation. Several observations highlight that PAs are also implicated in pathways regulating cell death. Indeed, the PA accumulation cytotoxic effect, maximized with the use of bovine serum amine oxidase (BSAO) enzyme, represents a valuable strategy against tumor progression. In the present study, along with the design, synthesis, and biological evaluation of a series of new spermine (Spm) analogues (1-23), a mixed structure-based (SB) and ligand-based (LB) protocol was applied. Binding modes of BSAO-PA modeled complexes led to clarify electrostatic and steric features likely affecting the BSAO-PA biochemical kinetics. LB and SB three-dimensional quantitative structure-activity relationship (Py-CoMFA and Py-ComBinE) models were developed by means of the 3d-qsar.com portal, and their analysis represents a strong basis for future design and synthesis of PA BSAO substrates for potential application in oxidative stress-induced chemotherapy.

DOI: https://doi.org/10.1021/acs.jcim.2c00559