bims-plasge Biomed News
on Plastid genes
Issue of 2024‒06‒16
two papers selected by
Vera S. Bogdanova, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
  1. Genetic diversity and population structure analysis of a diverse panel of pea (Pisum sativum).
  2. Active E. coli heteromeric acetyl-CoA carboxylase forms polymorphic helical tubular filaments.
  1. Front Genet. 2024 ;15 1396888
    Genetic diversity and population structure analysis of a diverse panel of pea (Pisum sativum).
    Haftom Brhane, Cecilia Hammenhag.
      Breeding resilient cultivars with increased tolerance to environmental stress and enhanced resistance to pests and diseases demands pre-breeding efforts that include understanding genetic diversity. This study aimed to evaluate the genetic diversity and population structure of 265 pea accessions. The diversity arrays technology (DArT) genotyping method was employed to identify single-nucleotide polymorphisms (SNPs) and silico markers. After stringent filtering, 6966 SNP and 8,454 silico markers were selected for diversity analysis. Genetic diversity was estimated by grouping accessions based on plant material type, geographic origin, growth habit, and seed color. Generally, diversity estimations obtained using SNPs were similar to those estimated using silico markers. The polymorphism information content (PIC) of the SNP markers ranged from 0.0 to 0.5, with a quarter of them displaying PIC values exceeding 0.4, making them highly informative. Analysis based on plant material type revealed narrow observed heterozygosity (Ho = 0.02-0.03) and expected heterozygosity (He = 0.26-0.31), with landrace accessions exhibiting the highest diversity. Geographic origin-based diversity analysis revealed Ho = 0.02-0.03 and He = 0.22 to 0.30, with European accessions showing the greatest diversity. Moreover, private alleles unique to landrace (4) and European (22) accessions were also identified, which merit further investigation for their potential association with desirable traits. The analysis of molecular variance revealed a highly significant genetic differentiation among accession groups classified by seed color, growth habit, plant material types, and geographic origin (p < 0.01). Principal coordinate analysis and neighbor-joining cluster analysis revealed weak clustering of accessions at different grouping levels. This study underscores the significance of genetic diversity in pea collections, offering valuable insights for targeted breeding and conservation efforts. By leveraging genomic data and exploring untapped genetic resources, pea breeding programs can be fortified to ensure sustainable plant protein production and address future challenges in agriculture.
    Keywords:  DArT; genetic diversity; pea; population genetic structure; single-nucleotide polymorphism
    DOI:  https://doi.org/10.3389/fgene.2024.1396888
  2. bioRxiv. 2024 May 28. pii: 2024.05.28.596234. [Epub ahead of print]
    Active E. coli heteromeric acetyl-CoA carboxylase forms polymorphic helical tubular filaments.
    Xueyong Xu, Amanda Silva de Sousa, Trevor J Boram, Wen Jiang, Jeremy R Lohman.
      The Escherichia coli heteromeric acetyl-CoA carboxylase (ACC) has four subunits assumed to form an elusive catalytic complex and are involved in allosteric and transcriptional regulation. The E. coli ACC represents almost all ACCs from pathogenic bacteria making it a key antibiotic development target to fight growing antibiotic resistance. Furthermore, it is a model for cyanobacterial and plant plastid ACCs as biofuel engineering targets. Here we report the catalytic E. coli ACC complex surprisingly forms tubes rather than dispersed particles. The cryo-EM structure reveals key protein-protein interactions underpinning efficient catalysis and how transcriptional regulatory roles are masked during catalysis. Discovering the protein-protein interaction interfaces that facilitate catalysis, allosteric and transcriptional regulation provides new routes to engineering catalytic activity and new targets for drug discovery.One-Sentence Summary: Bacterial heteromeric acetyl-CoA carboxylase forms tubes to promote efficient catalysis and mask transcriptional regulation.
    DOI:  https://doi.org/10.1101/2024.05.28.596234
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