bims-ovdlit Biomed News
on Ovarian cancer: early diagnosis, liquid biopsy and therapy
Issue of 2025–05–04
four papers selected by
Lara Paracchini, Humanitas Research



  1. Cancer Discov. 2025 Apr 29.
      Diagnostic delays in patients with brain cancer are common and can impact patient outcome. Development of a blood-based assay for detection of brain cancers could accelerate brain cancer diagnosis. In this study, we analyzed genome-wide cell-free (cfDNA) fragmentomes, including fragmentation profiles and repeat landscapes, from the plasma of individuals with (n=148) or without (n=357) brain cancer. Machine learning analyses of cfDNA fragmentome features detected brain cancer across all grade gliomas (AUC=0.90, 95% CI: 0.87-0.93) and these results were validated in an independent prospectively collected cohort. cfDNA fragmentome changes in patients with gliomas represented a combination of fragmentation profiles from glioma cells and altered white blood cell populations in the circulation. These analyses reveal the properties of cfDNA in patients with brain cancer and open new avenues for noninvasive detection of these individuals.
    DOI:  https://doi.org/10.1158/2159-8290.CD-25-0074
  2. Front Oncol. 2025 ;15 1574037
       Objective: BRCA-mutated women are recommended to undergo bilateral risk-reducing salpingo-oophorectomy (RRSO) after childbearing, due to the lack of effective methods that could be able to early detect the occurrence of ovarian cancer. Thus, predictive machine learning (ML) techniques could be crucial to aid clinicians in identifying high-risk BRCA-mutated patients and determining the appropriate timing for performing RRSO.
    Methods: In this work, we addressed this task by developing explainable ML models using clinical data referred to a multicentric cohort of 694 BRCA-mutated patients from six Italian centers (Policlinico Gemelli, IRCCS San Gerardo, Policlinico Bari, Istituto Tumori Regina Elena, Istituto Tumori Giovanni Paolo II, Ospedale F. Miulli), who performed salpingo-oophorectomy, out of which 39 patients showed tumor (5.6%). Data from Istituto Tumori Regina Elena and Policlinico Bari were used as External Validation Cohort (EVC). The other data were employed as Investigational Cohort (IC). Resampling and ensemble techniques were implemented to handle dataset imbalance. Explainable techniques enabled us to identify some protective and risk factors predicted by the models with respect to the task under study.
    Results: The best ML model achieved an AUC value of 79.3% (95% CI: 75.3% - 83.0%), an accuracy value of 73.8% (95% CI: 69.6% - 78.2%), a sensitivity value of 66.7% (95% CI: 58.1% - 75.3%), a specificity value of 74.3% (95% CI: 68.7% - 80.0%), and a G-mean value of 70.4% (95% CI: 63.0% - 76.0%) on EVC. Although the model demonstrated good overall performance, its limited sensitivity reduces its effectiveness in this high-risk population. The variables CA125, age and MatoRRSO were found to be the most significant risk factors, in agreement with the clinical perspective. Conversely, variables such as Estroprogestinuse and PregnancyNfdt played a protective factor role.
    Conclusion: Our ML proposal explores the intricate relationships between multiple clinical variables, with a particular emphasis on understanding their non-linear associations. However, while our approach provides valuable insights into risk assessment for BRCA-mutated patients, its current predictive capacity does not significantly improve upon existing clinical models.
    Keywords:  BRCA-mutated patients; artificial intelligence; machine learning; ovarian cancer risk; risk-reducing salpingo-oophorectomy
    DOI:  https://doi.org/10.3389/fonc.2025.1574037
  3. Clin Transl Oncol. 2025 Apr 30.
       BACKGROUND: BRCA1/2 Mutations have been linked to an inherited risk of breast and ovarian cancer. However, gene silencing by promoter methylation of BRCA1 and BRCA2 has not been studied extensively.
    MATERIALS AND METHODS: Promoter methylation of BRCA1 and BRCA2 in the gDNA of 113 hereditary breast and ovarian cancer patients was carried out using methylation-specific qPCR.
    RESULTS: The majority of patients showed higher methylation in BRCA2 than in BRCA1 and were significantly associated with hereditary breast and ovarian cancer Moreover, BRCA2 methylation was significantly associated with BRCA2 downregulation. Additionally, protein expression analysis in a subset of 25 patients with hypermethylated demonstrated a significant negative correlation between methylation status and protein expression for both BRCA1 and BRCA2.
    CONCLUSION: BRCA1 and BRCA2 promoter methylation, particularly BRCA2, contributes to gene silencing and protein loss, and may act as key biomarkers for hereditary breast and ovarian cancer prognosis and therapy.
    Keywords:  DNA methylation; Epigenetic events; Hereditary breast and ovarian cancer; Peripheral blood; Real time PCR
    DOI:  https://doi.org/10.1007/s12094-025-03934-w
  4. Proc Natl Acad Sci U S A. 2025 May 06. 122(18): e2505385122
      Aneuploidy is observed as gains or losses of whole chromosomes or chromosome arms and is a common hallmark of cancer. Whereas models for the generation of aneuploidy in cancer invoke mitotic chromosome segregation errors, whole-arm losses might occur simply as a result of centromere breakage. We recently showed that elevated RNA Polymerase II level over the S-phase-dependent histone genes predicts rapid recurrence of human meningioma and is correlated with total whole-arm losses relative to gains. To explain this imbalance in arm losses over gains, we have proposed that histone overexpression at S-phase competes with the histone H3 variant CENP-A, resulting in centromere breaks and whole-arm losses. To test whether centromere breaks alone can drive aneuploidy, we ask whether total whole-arm aneuploids can predict outcomes across different cancer types in large RNA and whole-genome sequencing databanks. We find that total whole-arm losses generally predict outcome, suggesting that centromere breakage is a major initiating factor leading to aneuploidy and the resulting changes in the selective landscape that drive most cancers. We also present evidence that centromere breakage alone is sufficient to account for whole-arm losses and gains, contrary to mitotic spindle error models for the generation of aneuploidy. Our results suggest that therapeutic intervention targeting histone overexpression has the potential to reduce aneuploidy and slow cancer progression.
    Keywords:  RNA sequencing; aneuploidy; centromeres; histones; whole genome sequencing
    DOI:  https://doi.org/10.1073/pnas.2505385122