bims-nocaut 2023-09-10 papers

Issue of 2023–09–10
three papers selected by
Quentin Frenger, University of Strasbourg

Autophagy. 2023 Sep 07. 1-2

The separate axes of TECPR1 and ATG16L1 in CASM.

Namrita Kaur, Sven R Carlsson, Alf Håkon Lystad.

Conjugation of ATG8 to single membranes (CASM) is a fundamental cellular process that entails the conjugation of mammalian Atg8 homologs, here referred to as ATG8, to phosphatidylethanolamine (PE) and phosphatidylserine (PS) on endolysosomal compartments. Our current research, together with recent reports from the Randow, Wu, and Wileman labs, has uncovered yet another layer to this process. We discovered that, in addition to ATG16L1-containing complexes, TECPR1 (tectonin beta-propeller repeat containing 1)-containing ATG12-ATG5 E3 complexes can facilitate CASM, thereby providing a broader understanding of this pathway.

Keywords: CASM; DysF; SopF; membrane damage; non-canonical autophagy; sphingomyelin

DOI: https://doi.org/10.1080/15548627.2023.2255462

Stress Biol. 2023 Jun 26. 3(1): 19

Rescuing the Golgi from heat damages by ATG8: restoration rather than clean-up.

Anni Luo, Jian-Xiang Liu.

High temperature stress poses significant adverse effects on crop yield and quality. Yet the molecular mechanisms underlying heat stress tolerance in plants/crops, especially regarding the organellar remodeling and homeostasis, are largely unknown. In a recent study, Zhou et al. reported that autophagy-related 8 (ATG8), a famous regulator involved in autophagy, plays a new role in Golgi restoration upon heat stress. Golgi apparatus is vacuolated following short-term acute heat stress, and ATG8 is translocated to the dilated Golgi membrane and interacts with CLATHRIN LIGHT CHAIN 2 (CLC2) to facilitate Golgi restoration, which is dependent on the ATG conjugation system, but not of the upstream autophagic initiators. These exciting findings broaden the fundamental role of ATG8, and elucidate the organelle-level restoration mechanism of Golgi upon heat stress in plants.

Keywords: ATG8; Autophagy; CLC2; ER; Golgi restoration; Heat stress

DOI: https://doi.org/10.1007/s44154-023-00100-6

Autophagy. 2023 Sep 08. 1-15

The nonautophagic functions of autophagy-related proteins.

Jia-Ni Shang, Chen-Ge Yu, Rui Li, Yan Xi, Yue Jenny Jian, Nan Xu, Su Chen.

ABBREVIATIONS: ATG: autophagy related; BECN1: beclin 1; cAMP: cyclic adenosine monophosphate; dsDNA: double-stranded DNA; EMT: epithelial-mesenchymal transition; IFN: interferon; ISCs: intestinal stem cells; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MAPK/JNK: mitogen-activated protein kinase/c-Jun N-terminal kinases; MTOR: mechanistic target of rapamycin kinase; STING1: stimulator of interferon response cGAMP interactor 1; UVRAG: UV radiation resistance associated; VPS: vacuolar protein sorting.

Keywords: autophagy-related proteins; cargo transport; cell growth and senescence; metabolic homeostasis; nonautophagic functions; signal transduction

DOI: https://doi.org/10.1080/15548627.2023.2254664