bims-mosdis Biomed News
on Mosquito distribution and disease
Issue of 2022–04–24
eightteen papers selected by
Richard Halfpenny, Staffordshire University



  1. Malar J. 2022 Apr 18. 21(1): 125
       BACKGROUND: Anopheles arabiensis, member species of the Anopheles gambiae complex, is the primary vector of malaria and is widely distributed in Ethiopia. Anopheles funestus, Anopheles pharoensis and Anopheles nili are secondary vectors occurring with limited distribution in the country. Indoor residual spraying (IRS) and long-lasting insecticidal nets (LLINs) are pillars for the interventions against malaria control and elimination efforts in Ethiopia. However, the emergence and widespread of insecticide resistance in An. gambiae sensu lato (s.l.), might compromise the control efforts of the country. The aim of this study was to investigate composition of mosquito fauna and insecticide resistance status of An. gambiae s.l. in Itang special district ( woreda), Gambella, southwestern Ethiopia.
    METHODS: Adult mosquitoes were sampled from September 2020 to February 2021 using the CDC light trap and pyrethrum spray catch (PSC). CDC light traps were placed in three selected houses for two consecutive days per month to collect mosquitoes indoor and outdoor from 6:00 P.M. to 06:00 A.M. and PSC was used to collect indoor resting mosquitoes from ten selected houses once in a month from October 2020 to February 2021. Moreover, mosquito larvae were also collected from different breeding sites and reared to adults to assess susceptibility status of populations of An. gambiae s.l. in the study area. Susceptibility tests were conducted on two to three days old non blood fed female An. gambiae s.l. using insecticide impregnated papers with deltamethrin (0.05%), alpha-cypermethrin (0.05%), propoxur (0.1%), pirimiphos-methyl (0.25%) and bendiocarb (0.1%) following World Health Organization (WHO) standard susceptibility test procedure. Molecular diagnostics were done for the identification of member species of An. gambiae s.l. and detection of knockdown resistance (kdr) allele using species specific polymerase chain reaction (PCR) and allele specific PCR.
    RESULTS: In total, 468 adult mosquitoes were collected from different houses. Culex mosquitoes were the most dominant (80.4%) followed by Anopheles mosquitoes. Three species of Anopheles (Anopheles coustani, An. pharoensis, and An. gambiae s.l.) were identified, of which An. coustani was the dominant (8.1%) species. Higher number of mosquitoes (231) were collected outdoor by CDC light traps. Out of 468 adult mosquitoes, 294 were blood fed, 46 were half-gravid and gravid whereas the remaining 128 were unfed. WHO bioassay tests revealed that the populations of An. gambiae s.l. in the study area are resistant against alpha-cypermethrin and deltamethrin, but susceptible to bendiocarb, pirimiphos-methyl and propoxur. Of the total 86 An. gambiae s.l. specimens assayed, 79 (92%) successfully amplified and identified as An. arabiensis. West African kdr (L1014F) mutation was detected with high kdr allele frequency ranging from 67 to 88%.
    CONCLUSION: The detection of target site mutation, kdr L1014F allele, coupled with the phenotypic resistance against alpha-cypermethrin and deltamethrin call for continuous resistance monitoring.
    Keywords:  Anopheles mosquito; Ethiopia; Insecticide resistance; Itang; Malaria
    DOI:  https://doi.org/10.1186/s12936-022-04150-5
  2. Parasit Vectors. 2022 Apr 19. 15(1): 133
       BACKGROUND: Mosquito control is currently the main tool available to contain the spread of several arboviruses in Brazil. We have evaluated the association between entomological surveys of female adult Aedes aegypti and the Breteau index (BI) in space and time in a hyperendemic area, and compared the human resources costs required to measure each of these indicators.
    METHODS: Entomological surveys were conducted between 2016 and 2019 in Vila Toninho, a neighborhood in the city of São José do Rio Preto, Brazil. Monthly records of collected mosquito specimens were made and then grouped by season.
    RESULTS: Our findings showed that adult and immature mosquitoes are more related in time than in space, possibly due to differences in their habitats or in climate variables. Bayesian temporal modeling revealed that an increase in 1 standard deviation in the BI was associated with a 27% increase in the number of adult female mosquitoes when adjusted for climatic conditions. The cost of entomological surveys of adult mosquitoes was found to be 83% lower than the cost of determining the BI when covering the same geographic area.
    CONCLUSIONS: For fine-scale assessments, a simple measure of adult Ae. aegypti abundance may be more realistic than aquatic indicators, but the adult indices are not necessarily the only reliable measure. Surveying adult female mosquitoes has significant potential for optimizing vector control strategies because, unlike the BI, this tool provides an effective indicator for micro-areas within an urban region. It should be noted that the results of the present study may be due to specific features of of the study area, and future studies should analyze whether the patterns found in the study neighborhood are also found in other regions.
    Keywords:  Bayesian analysis; Breteau index; Entomological index; Mosquito
    DOI:  https://doi.org/10.1186/s13071-022-05244-4
  3. Acta Trop. 2022 Apr 14. pii: S0001-706X(22)00162-0. [Epub ahead of print] 106470
      West Nile virus (WNV) infections have increased over recent years to the extent that WNV has become one of the most widespread arboviruses in the world, with potential consequences for both human and animal health. While much is known about WNV and the vectors that transmit it from their primary hosts across continental Europe, little is known about the epidemiology of the disease on the island of Cyprus. In this study, the aim was to investigate the prevalence of WNV infection in potential mosquito vectors for the first time in the Republic of Cyprus, using WNV surveillance of mosquitoes. Mosquitoes were collected in 2019, during which an outbreak in humans had occurred, and sampled mosquitoes were then examined for WNV infection by testing them for the presence of WNV RNA. Of 126 mosquito pools tested, one pool, containing Culex pipiens mosquitoes sampled from the Nicosia district, was found to be positive for the presence of WNV RNA. The positive pool found in this study represents the first demonstration of WNV in mosquitoes in Cyprus and confirms that human cases in Cyprus are likely the result of transmission via local Culex mosquitoes.
    Keywords:  Culex pipiens, vector-borne infections; Cyprus; West Nile virus; mosquitoes; real-time PCR
    DOI:  https://doi.org/10.1016/j.actatropica.2022.106470
  4. Pathogens. 2022 Mar 31. pii: 423. [Epub ahead of print]11(4):
      Mosquitoes breeding in urban sewage infrastructure are both a source of nuisance to the local population and a public health risk, given that biting mosquitoes can transmit pathogenic organisms to humans. The increasing presence of the invasive mosquito species Aedes albopictus in European cities has further exacerbated the problems already caused by native Culex pipiens. We tested the effectiveness of modifications to sewage structures as an alternative to the use of biocides to prevent mosquito breeding. The placing of a layer of concrete at the bottom of sand sewers to prevent water accumulation completely eliminated mosquito reproduction, and so eliminates the need for biocides in modified structures. Sewer modification is thus a valid low-cost alternative for mosquito control.
    Keywords:  Aedes albopictus; Culex pipiens; integrated mosquito management; invasive Aedes; manipulation of mosquito habitat; mosquito control
    DOI:  https://doi.org/10.3390/pathogens11040423
  5. Viruses. 2022 Mar 27. pii: 691. [Epub ahead of print]14(4):
      The geographic expansion of mosquitos is associated with a rising frequency of outbreaks of mosquito-borne diseases (MBD) worldwide. We collected occurrence locations and times of mosquito species, mosquito-borne arboviruses, and MBDs in the mainland of China in 1954-2020. We mapped the spatial distributions of mosquitoes and arboviruses at the county level, and we used machine learning algorithms to assess contributions of ecoclimatic, socioenvironmental, and biological factors to the spatial distributions of 26 predominant mosquito species and two MBDs associated with high disease burden. Altogether, 339 mosquito species and 35 arboviruses were mapped at the county level. Culex tritaeniorhynchus is found to harbor the highest variety of arboviruses (19 species), followed by Anopheles sinensis (11) and Culex pipiens quinquefasciatus (9). Temperature seasonality, annual precipitation, and mammalian richness were the three most important contributors to the spatial distributions of most of the 26 predominant mosquito species. The model-predicted suitable habitats are 60-664% larger in size than what have been observed, indicating the possibility of severe under-detection. The spatial distribution of major mosquito species in China is likely to be under-estimated by current field observations. More active surveillance is needed to investigate the mosquito species in specific areas where investigation is missing but model-predicted probability is high.
    Keywords:  China; arboviruses; distribution; mosquito-borne diseases; mosquitoes; risk determinants
    DOI:  https://doi.org/10.3390/v14040691
  6. PLoS One. 2022 ;17(4): e0266736
       BACKGROUND: Long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) are the main malaria vector control measures deployed in Kenya. Widespread pyrethroid resistance among the primary vectors in Western Kenya has necessitated the re-introduction of IRS using an organophosphate insecticide, pirimiphos-methyl (Actellic® 300CS), as a pyrethroid resistance management strategy. Evaluation of the effectiveness of the combined use of non-pyrethroid IRS and LLINs has yielded varied results. We aimed to evaluate the effect of non-pyrethroid IRS and LLINs on malaria indicators in a high malaria transmission area.
    METHODS: We reviewed records and tallied monthly aggregate of outpatient department (OPD) attendance, suspected malaria cases, those tested for malaria and those testing positive for malaria at two health facilities, one from Nyatike, an intervention sub-county, and one from Suba, a comparison sub-county, both located in Western Kenya, from February 1, 2016, through March 31, 2018. The first round of IRS was conducted in February-March 2017 in Nyatike sub-county and the second round one year later in both Nyatike and Suba sub-counties. The mass distribution of LLINs has been conducted in both locations. We performed descriptive analysis and estimated the effect of the interventions and temporal changes of malaria indicators using Poisson regression for a period before and after the first round of IRS.
    RESULTS: A higher reduction in the intervention area in total OPD, the proportion of OPD visits due to suspected malaria, testing positivity rate and annual malaria incidences were observed except for the total OPD visits among the under 5 children (59% decrease observed in the comparison area vs 33% decrease in the intervention area, net change -27%, P <0.001). The percentage decline in annual malaria incidence observed in the intervention area was more than twice the observed percentage decline in the comparison area across all the age groups. A marked decline in the monthly testing positivity rate (TPR) was noticed in the intervention area, while no major changes were observed in the comparison area. The monthly TPR reduced from 46% in February 2016 to 11% in February 2018, representing a 76% absolute decrease in TPR among all ages (RR = 0.24, 95% CI 0.12-0.46). In the comparison area, TPR was 16% in both February 2016 and February 2018 (RR = 1.0, 95% CI 0.52-2.09). A month-by-month comparison revealed lower TPR in Year 2 compared to Year 1 in the intervention area for most of the one year after the introduction of the IRS.
    CONCLUSIONS: Our findings demonstrated a reduced malaria burden among populations protected by both non-pyrethroid IRS and LLINs implying a possible additional benefit afforded by the combined intervention in the malaria-endemic zone.
    DOI:  https://doi.org/10.1371/journal.pone.0266736
  7. BMC Med. 2022 Apr 20. 20(1): 135
       BACKGROUND: Sub-Saharan Africa has seen substantial reductions in cases and deaths due to malaria over the past two decades. While this reduction is primarily due to an increasing expansion of interventions, urbanisation has played its part as urban areas typically experience substantially less malaria transmission than rural areas. However, this may be partially lost with the invasion and establishment of Anopheles stephensi. A. stephensi, the primary urban malaria vector in Asia, was first detected in Africa in 2012 in Djibouti and was subsequently identified in Ethiopia in 2016, and later in Sudan and Somalia. In Djibouti, malaria cases have increased 30-fold from 2012 to 2019 though the impact in the wider region remains unclear.
    METHODS: Here, we have adapted an existing model of mechanistic malaria transmission to estimate the increase in vector density required to explain the trends in malaria cases seen in Djibouti. To account for the observed plasticity in An. stephensi behaviour, and the unknowns of how it will establish in a novel environment, we sample behavioural parameters in order to account for a wide range of uncertainty. This quantification is then applied to Ethiopia, considering temperature-dependent extrinsic incubation periods, pre-existing vector-control interventions and Plasmodium falciparum prevalence in order to assess the potential impact of An. stephensi establishment on P. falciparum transmission. Following this, we estimate the potential impact of scaling up ITN (insecticide-treated nets)/IRS (indoor residual spraying) and implementing piperonyl butoxide (PBO) ITNs and larval source management, as well as their economic costs.
    RESULTS: We estimate that annual P. falciparum malaria cases could increase by 50% (95% CI 14-90) if no additional interventions are implemented. The implementation of sufficient control measures to reduce malaria transmission to pre-stephensi levels will cost hundreds of millions of USD.
    CONCLUSIONS: Substantial heterogeneity across the country is predicted and large increases in vector control interventions could be needed to prevent a major public health emergency.
    Keywords:  Anopheles stephensi; Ethiopia; Insecticide; Invasive; Malaria; Mathematical modelling; Vector control
    DOI:  https://doi.org/10.1186/s12916-022-02324-1
  8. Acta Trop. 2022 Apr 14. pii: S0001-706X(22)00163-2. [Epub ahead of print] 106471
      Aedes aegypti (L.) (Diptera: Culicidae) survives in the egg stage under unfavorable environmental conditions. In this study, we assessed the survival of Ae. aegypti eggs under natural winter conditions as an overwintering mechanism. To address this, field collected eggs (from Buenos Aires city) were exposed for three months during the winter season in three sites of Santa Rosa and General Acha (La Pampa province, Argentina). Eggs survival and hatching response were analyzed considering two factors (site of exposure and time of oviposition). Of the 1397 analyzed eggs, 936 (67%) were viable at the end of the study. Egg survival showed to be high in all sites (between 53% and 84%), except in one site of Santa Rosa. Also, eggs survival was higher in eggs laid in May (94%) (late-fall) as compared to those laid in March (61%) and April (56%) (early- and mid-fall respectively). Eggs hatching response was only affected by the time of oviposition, being lower for eggs laid in May (33%) as compared to March (38%) and April (50%). These results provide information regarding the winter resistance of Ae. aegypti at the limit of its distribution in temperate regions. Given the high percentages of survival found in this study, we consider that the low winter temperatures would not be a limitation for its establishment in southern areas.
    Keywords:  Argentina; egg resistance; hatching response; mosquito vector; temperate regions
    DOI:  https://doi.org/10.1016/j.actatropica.2022.106471
  9. Behav Sci (Basel). 2022 Mar 28. pii: 94. [Epub ahead of print]12(4):
      Knowledge of dengue fever and perceived self-efficacy toward dengue prevention does not necessarily translate to the uptake of mosquito control measures. Understanding how these factors (knowledge and self-efficacy) influence mosquito control measures in Mexico is limited. Our study sought to bridge this knowledge gap by assessing individual-level variables that affect the use of mosquito control measures. A cross-sectional survey with 623 participants was administered online in Mexico from April to July 2021. Multiple linear regression and multiple logistic regression models were used to explore factors that predicted mosquito control scale and odds of taking measures to control mosquitoes in the previous year, respectively. Self-efficacy (β = 0.323, p-value = &lt; 0.0001) and knowledge about dengue reduction scale (β = 0.316, p-value =&lt; 0.0001) were the most important predictors of mosquito control scale. The linear regression model explained 24.9% of the mosquito control scale variance. Increasing age (OR = 1.064, p-value =&lt; 0.0001) and self-efficacy (OR = 1.020, p-value = 0.0024) were both associated with an increase in the odds of taking measures against mosquitoes in the previous year. There is a potential to increase mosquito control awareness and practices through the increase in knowledge about mosquito reduction and self-efficacy in Mexico.
    Keywords:  Colima; Mexico; Sonora; dengue fever; knowledge; mosquito control; prevention; self-efficacy
    DOI:  https://doi.org/10.3390/bs12040094
  10. Insects. 2022 Mar 31. pii: 347. [Epub ahead of print]13(4):
      Sterile males of Aedes aegypti were released once a week for 8 weeks to evaluate the dispersal efficiency of ground and aerial drone release methods in a rural village of 26 Ha in southern Mexico. Indoor and outdoor BG-Sentinel traps were placed in 13-16 houses distributed throughout the village. The BG traps were activated 48 h after the release of the sterile males and functioned for a 24 h period following each release. Over the 8-week period of simultaneous ground and aerial releases, an average of 85,117 ± 6457 sterile males/week were released at ground level and 86,724 ± 6474 sterile males/week were released using an aerial drone. The ground release method resulted in higher numbers of captured males (mean = 5.1 ± 1.4, range 1.1-15.7 sterile males/trap) compared with the aerial release method (mean = 2.6 ± 0.8, range 0.5-7.3 sterile males/trap) (p &lt; 0.05). Similarly, the prevalence of traps that captured at least one sterile male was significantly higher for ground release compared to the aerial release method (p &lt; 0.01). The lower numbers of sterile males captured in the aerial release method could be due to mortality or physical injury caused by the chilling process for immobilization, or the compaction of these insects during transport and release. However, aerial releases by a two-person team distributed insects over the entire village in just 20 min, compared to ~90 min of work for a five-person team during the ground release method. Ground release also resulted in higher aggregations of males and some villagers reported feeling discomfort from the presence of large numbers of mosquitoes in and around their houses. We conclude that modifications to the handling and transport of sterile males and the design of containers used to store males are required to avoid injury and to improve the efficiency of aerial releases for area-wide SIT-based population suppression programs targeted at mosquito vectors of human disease.
    Keywords:  physical injury; recapture rates; release techniques; release time; sterile insect technique; vector control
    DOI:  https://doi.org/10.3390/insects13040347
  11. Ecohealth. 2022 Apr 19.
      Identification and classification of high-risk areas for the presence of Aedes aegypti is not an easy task. To develop suitable methods to identify this areas is an essential task that will increase the efficiency and effectiveness of control measures and to optimize the use of resources. The objectives of this study were to identify high- risk areas for the presence of Ae. aegypti using mosquito traps and household visits to identify breeding sites; to identify and validate aspects of the remote sensing images that could characterize these areas; to evaluate the relationship between this spatial risk classification and the occurrence of Ae. aegypti; and provide a methodology to the health and control vector services and prioritize these areas for development of control measure. Information about the geographical coordinates of these traps will enable us to apply the kriging spatial analysis tool to generate maps with the predicted numbers of Ae. aegypti. Satellite images were used to identify the characteristic features the four areas, so that other areas could also be classified using only the sensing remote images. The developed methodology enables the identification of high-risk areas for Ae. aegypti and for the occurrence of Dengue, as well as Zika fever and Chikungunya fever using only sensing remote images. These results allow health and vector control services to prioritize these areas for developing surveillance and control measures. The use of the available resources can be optimized and potentially promote a decrease in the expected incidences of these diseases, particularly Dengue.
    Keywords:  Aedes aegypti; Ordinary Kriging; Remote Sensing; Traps
    DOI:  https://doi.org/10.1007/s10393-022-01581-z
  12. Am J Trop Med Hyg. 2022 Apr 18. pii: tpmd200669. [Epub ahead of print]
      Dengue is a public health problem in Colombia and in the municipality of Girardot, an area of high risk for dengue transmission. We present the results of an economic evaluation from the societal perspective and 1-year time horizon comparing the regular control program for dengue prevention versus an intervention that comprised an environmental management strategy by covering the most Aedes aegypti productive breeding sites with insecticide covers, community actions, and educational activities. The effectiveness of the intervention was measured as the reduction in probability of dengue infection obtained from a community trial. Resource use was estimated from clinical records that were validated by clinical experts; unit costs were taken from national tariffs. Patient costs were obtained from a household survey. We found that the intervention generated an additional cost of USD20.9 per household and an incremental effectiveness of 0.00173 (reduction in the probability of reported dengue cases). Overall, both alternatives generate similar effectiveness, but the new intervention was associated with increasing costs. We conclude the new intervention is a potentially cost-effective option in areas where high prevalence of dengue exists.
    DOI:  https://doi.org/10.4269/ajtmh.20-0669
  13. Emerg Infect Dis. 2022 May;28(5): 1071-1074
      We tested 294 domestic pet dogs in Mexico for neutralizing antibodies for mosquito-borne flaviviruses. We found high (42.6%) exposure to West Nile virus in Reynosa (northern Mexico) and low (1.2%) exposure in Tuxtla Gutierrez (southern Mexico) but very limited exposure to Aedes-borne flaviviruses. Domestic dogs may be useful sentinels for West Nile virus.
    Keywords:  Mexico; West Nile virus; Zika virus; arboviruses; dengue virus; dogs; flaviviruses; pets; vector-borne infections; viruses
    DOI:  https://doi.org/10.3201/eid2805.211879
  14. Acta Trop. 2022 Apr 14. pii: S0001-706X(22)00144-9. [Epub ahead of print] 106447
      Mosquito-borne diseases are emerging and re-emerging across the globe, especially after the COVID19 pandemic. The recent advances in text mining in infectious diseases hold the potential of providing timely access to explicit and implicit associations among information in the text. In the past few years, the availability of online text data in the form of unstructured or semi-structured text with rich content of information from this domain enables many studies to provide solutions in this area, e.g., disease-related knowledge discovery, disease surveillance, early detection system, etc. However, a recent review of text mining in the domain of mosquito-borne disease was not available to the best of our knowledge. In this review, we survey the recent works in the text mining techniques used in combating mosquito-borne diseases. We highlight the corpus sources, technologies, applications, and the challenges faced by the studies, followed by the possible future directions that can be taken further in this domain. We present a bibliometric analysis of the 294 scientific articles that have been published in Scopus and PubMed in the domain of text mining in mosquito-borne diseases, from the year 2016 to 2021. The papers were further filtered and reviewed based on the techniques used to analyze the text related to mosquito-borne diseases. Based on the corpus of 158 selected articles, we found 27 of the articles were relevant and used text mining in mosquito-borne diseases. These articles covered the majority of Zika (38.70%), Dengue (32.26%), and Malaria (29.03%), with extremely low numbers or none of the other crucial mosquito-borne diseases like chikungunya, yellow fever, West Nile fever. Twitter was the dominant corpus resource to perform text mining in mosquito-borne diseases, followed by PubMed and LexisNexis databases. Sentiment analysis was the most popular technique of text mining to understand the discourse of the disease and followed by information extraction, which dependency relation and co-occurrence-based approach to extract relations and events. Surveillance was the main usage of most of the reviewed studies and followed by treatment, which focused on the drug-disease or symptom-disease association. The advance in text mining could improve the management of mosquito-borne diseases. However, the technique and application posed many limitations and challenges, including biases like user authentication and language, real-world implementation, etc. We discussed the future direction which can be useful to expand this area and domain. This review paper contributes mainly as a library for text mining in mosquito-borne diseases and could further explore the system for other neglected diseases.
    Keywords:  Dengue; Malaria; Mosquito-borne; Text Analysis; Vector-borne; Zika
    DOI:  https://doi.org/10.1016/j.actatropica.2022.106447
  15. Malar J. 2022 Apr 22. 21(1): 128
       BACKGROUND: Countries in malaria endemic regions are determinedly making an effort to achieve the global malaria elimination goals. In Ethiopia, too, all concerned bodies have given attention to this mission as one of their priority areas so that malaria would be eradicated from the country. Despite the success stories from some areas in the country, however, malaria is still a major public health concern in most parts of Ethiopia. Therefore, this study is aimed at analysing the changing malaria trend and assessing the impact of malaria control efforts in one of the malaria endemic regions of Ethiopia.
    METHODS: Five years data on clinical malaria cases diagnosed and treated at all health facilities (including 28 Health Centres, 105 Health Posts and 2 Hospitals) in Oromia Special zone, Amhara Regional State, Ethiopia, were reviewed for the period from June 2014 to June 2019. Data on different interventional activities undertaken in the zone during the specified period were obtained from the Regional Health Bureau.
    RESULTS: The cumulative malaria positivity rate documented in the zone was 12.5% (n = 65,463/524,722). Plasmodium falciparum infection was the dominant malaria aetiology and accounted for 78.9% (n = 51,679). The age group with the highest malaria burden was found to be those aged above 15 years (54.14%, n = 35,443/65,463). The malaria trend showed a sharp decreasing pattern from 19.33% (in 2015) to 5.65% (in 2018), although insignificant increment was recorded in 2019 (8.53%). Distribution of long-lasting insecticidal nets (LLIN) and indoor residual spraying (IRS) were undertaken in the zone once a year only for two years, specifically in 2014 and 2017. In 2014, a single LLIN was distributed per head of households, which was not sufficient for a family size of more than one family member. Number of houses sprayed with indoor residual spray in 2014 and 2017 were 33,314 and 32,184 houses, respectively, leading to the assumption that, 151,444 (25.9%) and 141,641 (24.2%) population were protected in year 2014 and 2017, respectively. The analysis has shown that P. falciparum positivity rate was significantly decreased following the interventional activities by 3.3% (p = 0.009), but interventional efforts did not appear to have significant effect on vivax malaria, as positivity rate of this parasite increased by 1.49% (p = 0.0218).
    CONCLUSION: Malaria burden has shown a decreasing pattern in the study area, although the pattern was not consistent throughout all the years and across the districts in the study area. Therefore, unremitting surveillance along implementation of interventional efforts should be considered taking into account the unique features of Plasmodium species, population dynamics in the zone, seasonality, and malaria history at different districts of the zone should be in place to achieve the envisaged national malaria elimination goal by 2030.
    Keywords:  IRS; LLIN; Malaria; Oromia Special zone; Plasmodium falciparum; Plasmodium vivax; Prevalence
    DOI:  https://doi.org/10.1186/s12936-022-04149-y
  16. Euro Surveill. 2022 Apr;27(16):
      We report an outbreak investigation of two fatal cases of autochthonous Plasmodium falciparum malaria that occurred in Belgium in September 2020. Various hypotheses of the potential source of infection were investigated. The most likely route of transmission was through an infectious exotic Anopheles mosquito that was imported via the international airport of Brussels or the military airport Melsbroek and infected the cases who lived at 5 km from the airports. Based on genomic analysis of the parasites collected from the two cases, the most likely origin of the Plasmodium was Gabon or Cameroon. Further, the parasites collected from the two Belgian patients were identical by descent, which supports the assumption that the two infections originated from the bite of the same mosquito, during interrupted feeding. Although airport malaria remains a rare event, it has significant implications, particularly for the patient, as delayed or missed diagnosis of the cause of illness often results in complications and mortality. Therefore, to prevent such severe or fatal outcomes, we suggest a number of public health actions including increased awareness among health practitioners, especially those working in the vicinity of airports, and increased surveillance of exotic mosquito species at airports.
    Keywords:  Belgium; Odyssean malaria; Plasmodium falciparum; airport malaria; autochthonous transmission; malaria
    DOI:  https://doi.org/10.2807/1560-7917.ES.2022.27.16.2100724
  17. Malar J. 2022 Apr 19. 21(1): 127
       BACKGROUND: Roraima state is the northernmost state in Brazil and the primary border-crossing point between Brazil and Venezuela. The uncontrolled surge of malaria in Venezuela, coupled with mass migration of Venezuelans to neighbouring countries and the upward trend in informal mining in the state, pose a serious threat to the broader region, especially to migrant, indigenous and mining populations, jeopardizing malaria elimination efforts. This study describes changes in the epidemiological profile of malaria in Roraima state related to time, place and populations at risk from 2016 to 2020.
    METHODS: De-identified malaria surveillance data were obtained from the Malaria Epidemiological Surveillance System from 2016 to 2020. Pearson's chi-square tested differences between imported and autochthonous cases. Multivariable logistic regression was used to identify risk factors for imported versus autochthonous cases by demographic characteristics.
    RESULTS: Odds of being an imported case were higher for Plasmodium falciparum cases (AOR = 2.08). However, as the number of cases from Venezuela decreased in 2020 following closure of the border, the proportion of P. falciparum cases increased markedly, from 6.24% in 2019 to 18.50% in 2020. Over the 5-year period, the odds of being an imported case among miners were about nine times higher than the general population (AOR = 8.99). The proportion of total malaria cases that were among indigenous people increased from 33.09% in 2016 to 54.83% in 2020. Indigenous children had a higher burden of malaria with over 40% of cases in children 0 to 9 years old, compared to 8% in non-indigenous children 0 to 9 years old. In some municipalities, place of infection differed from place of notification, with a large proportion of cases in these municipalities reporting in Boa Vista.
    CONCLUSIONS: Malaria remains a serious threat in Roraima state, especially among high-risk populations, such as miners, migrants, and indigenous people. As malaria cases have increased among indigenous people and miners, and the proportion of P. falciparum cases has increased, elimination efforts require understanding of these risk factors to tailor interventions appropriately. Furthermore, cross-border surveillance systems need to be urgently strengthened at formal and unofficial border points, especially since the border with Venezuela reopened in July 2021.
    Keywords:  Brazil; Cross-border malaria; High risk groups; Imported malaria; Indigenous groups; Key populations; Migrant populations; Mining malaria; Roraima
    DOI:  https://doi.org/10.1186/s12936-022-04141-6
  18. J Med Life. 2022 Mar;15(3): 392-396
      Malaria is the most severe protozoan disease in the world. As a result of strict malaria control programs, malaria's epidemiological model has changed. Knowing this epidemiological model and its effects will help us predict and prevent a new epidemic. This research was conducted to review the epidemiological trend of malaria in the Kermanshah province of Iran and some of its effective factors. Data were extracted from the registers in the disease control unit of the province, national population census, and annual rainfall report. The data was processed by SPSS16. There has been an evident decrease in malaria cases over the last 30 years in Kermanshah. This decreasing trend began especially after 1994, and since then, just 6% of all cases have happened. Between 1990 to 1997, an epidemic occurred, and more than 80% of positive cases were registered in these years. P. vivax malaria was the most common type (99.32%), and P. falciparum malaria was the second, with a very egregious difference (0.68%). The average age was 23.1 years, and men were infected twice more than women. There was a positive relation between annual rainfall level and positive malaria cases in these cities. After the major changes in malaria control programs in Kermanshah province, the number of positive cases fell noticeably, and now it is in the elimination stage. All cases of malaria were imported in the last years, and no resistance type was ever seen.
    Keywords:  Kermanshah; epidemiologic trend; malaria; plasmodium SP
    DOI:  https://doi.org/10.25122/jml-2021-0374