bims-mistre Biomed News
on Mito stress
Issue of 2026–03–22
fourteen papers selected by
Ellen Siobhan Mitchell, MitoQ



  1. Prev Nutr Food Sci. 2026 Feb;pii: pnf.2025.231. [Epub ahead of print]31(1):
      Purslane (Portulaca oleracea L.) exhibits anti-inflammatory and antioxidant properties, but its role in regulating adipose tissue inflammatory microRNAs and mitochondrial biogenesis in skeletal muscle under high-fat diet (HFD) conditions remains unclear. This study aimed to evaluate the effects of purslane extract (PE) on HFD-induced inflammation and mitochondrial biogenesis, focusing on regulation of the miR-221/222 cluster in white adipose tissue (WAT) and the AMP-activated protein kinase (AMPK) and sirtuin 1 (SIRT1) axis in skeletal muscle. Male Sprague-Dawley rats were divided into four groups: normal chow diet, HFD, HFD+0.2% PE, and HFD+0.4% PE for 12 weeks. PE supplementation significantly reduced body weight gain, improved serum and hepatic lipid profiles, and decreased circulating inflammatory cytokines (P<0.05). In WAT, PE reduced expression of pro-inflammatory cytokines and M1 macrophage markers while increasing expression of a M2 marker. Additionally, PE inhibited phospho-nuclear factor-kappa B levels and suppressed miR-221/222 expression in WAT. In skeletal muscle, PE enhanced the expression of genes related to mitochondrial biogenesis and restored mitochondrial content. Furthermore, PE increased AMPK, total SIRT, and SIRT1 activities, supporting its role in improving mitochondrial function in skeletal muscle. These findings suggest that the beneficial effects of PE against HFD-induced obesity are mediated, at least in part, by suppression of the WAT miR-221/222 cluster and activation of the AMPK/SIRT1 axis in skeletal muscle, thereby alleviating inflammation and enhancing mitochondrial biogenesis.
    Keywords:  Portulaca; inflammation; microRNAs; mitochondria; obesity
    DOI:  https://doi.org/10.3746/pnf.2025.231
  2. J Am Geriatr Soc. 2026 Mar 16.
      GDF15 signals energetic stress to the brain, leading to unpleasant symptoms as the body conserves and reallocates energy. In conditions such as frailty and cancer, suppression of GDF15 signaling is expected to lead to an improvement in symptoms, but potentially at the cost of long-term health and survival.
    Keywords:  GDF15; clinical trial; energy; ponsegromab; side effect
    DOI:  https://doi.org/10.1111/jgs.70399
  3. iScience. 2026 Mar 20. 29(3): 114733
      Ovarian aging, marked by follicle depletion and oocyte quality decline, involves complex metabolic alterations. This review synthesizes evidence that dysregulated metabolic reprogramming, encompassing energy, lipid, and nutrient metabolism, drives ovarian functional decline. Central to this process is a self-reinforcing "metabolism-epigenetics-immunity" triangular network, where mitochondrial dysfunction and NAD+ depletion disrupt epigenetic regulation and activate chronic inflammation, collectively accelerating follicular atresia and hormonal dysfunction. By integrating this mechanistic framework, we highlight emerging intervention strategies targeting metabolic hubs, such as mitochondrial rescue and senescent cell clearance, which offer new avenues for preserving ovarian function. This work provides a conceptual foundation for developing personalized strategies to mitigate reproductive aging and its systemic health impacts.
    Keywords:  epigenetics; human metabolism; immunity
    DOI:  https://doi.org/10.1016/j.isci.2026.114733
  4. Genome Biol Evol. 2026 Mar 16. pii: evag067. [Epub ahead of print]
      Why do some species live for mere months, while others persist for centuries? A leading explanation implicates mitochondria. The mitochondrial theory of aging predicts that mitochondrial efficiency diminishes with age due to the accumulation of mutations within mitochondrial DNA (mtDNA). While experimental evidence for this theory is mixed, evolutionary analyses offer an ideal opportunity to determine if mitochondrial substitution rates are linked to longevity. Here, we explored the relationship between mtDNA evolution and species' lifespans across four clades-Aves, Actinopterygii, Bivalvia, and Sebastidae-using five normalization strategies. Across most methods, long-lived vertebrates showed reduced synonymous and nonsynonymous substitution rates, suggesting lower mtDNA mutation. However, we found that the strength and direction of these relationships varied drastically depending on the normalization approach used (i.e., correcting for divergence, generation time, and phylogeny). We also analyzed mtDNA mutation spectra and found similar patterns in long- and short-lived species, suggesting decreased rates of mtDNA mutations in long-lived species are not due to suppression of specific mutation processes, as predicted from the free-radical theory of aging. We also find little evidence for a relationship between selection on mitochondrial protein-coding genes and lifespan. Our results align with the idea that decreased mutation rates may help preserve mitochondrial integrity in long-lived vertebrate species, but that these species have not been selected to have particularly efficient OXPHOS or protection against a specific mitochondrial mutation process. Together, these findings underscore the critical link between mitochondrial stability and lifespan, and highlight the power of natural systems in this field.
    Keywords:  Mitochondrial DNA; comparative genomics; generation time; longevity; phylogenetic comparative methods; substitution rates
    DOI:  https://doi.org/10.1093/gbe/evag067
  5. Front Aging Neurosci. 2026 ;18 1729015
      Glutathione (GSH) is an abundant antioxidant that protects against endogenous and exogenous toxic agents. The evidence over the relationship between GSH and cognitive integrity during aging is still scarce. In this study we investigated the relationship between GSH and cognitive integrity, cognitive effort and sustained cognitive effort. Second, we explored whether GSH modulation is related to other physiological properties such as blood oxygenation (BOLD response) and to brain excitability (measured by GABA+ and Glx levels). We measured GSH levels through magnetic resonance spectroscopy (HERMES) at baseline and during cognitive task performance in 40 young (18-35 years; 26 female) and 40 older (60-85 years; 21 female) adults in two higher-order processing areas in the brain: the inferior frontal and the inferior parietal cortices (IFC and IPL). GSH in IPL related in opposite directions to distinct memory tasks in young and older adults. GSH levels in both regions showed a modulation as a result of sustained cognitive performance; the direction of this modulation was age- and region-dependent. Furthermore, GSH modulation positively related to cognitive performance in young adults. Finally, GSH showed a relationship with GABA that was region, age and state dependent. These results highlight the heterogeneity of GSH physiology, while its relation with cognition is dependent on age and brain region.
    Keywords:  GABA; GSH; HERMES; aging; cognition; glutamate; glutathione; magnetic resonance spectroscopy
    DOI:  https://doi.org/10.3389/fnagi.2026.1729015
  6. Inflammopharmacology. 2026 Mar 10.
      Ageing has been recognized as the leading risk factor for Alzheimer's disease (AD), with an intricate interplay of oxidative stress, neuroinflammation, and cellular senescence implicated in its pathogenesis. Mitochondrial dysfunction has been linked to redox imbalance and excessive production of reactive oxygen species (ROS), which disrupt homeostasis and damage both mitochondrial and nuclear DNA, thereby promoting amyloid-β accumulation and cognitive decline. Chronic activation of inflammasome signaling in microglia and astrocytes, characterized by the upregulation of NLRP3 and NF-κB, has been linked to the establishment of a neuroinflammatory environment, leading to synaptic loss and exacerbating tau pathology. Additionally, the accumulation of senescent glial and neuronal cells has been shown to drive the senescence-associated secretory phenotype (SASP), further amplifying inflammation and oxidative damage. Promising therapeutic interventions, including mitochondria-targeted antioxidants and senolytics, have been evaluated; however, translational challenges persist, such as the heterogeneity of biomarker measures and the insufficient delivery of antioxidants. A proposed roadmap emphasizes the importance of monitoring oxidative and inflammatory biomarkers, implementing combinatorial therapies, and personalizing interventions to enhance resilience in the ageing brain and delay the onset of AD.
    Keywords:  Alzheimer’s disease; Cytokines; Neurodegeneration; Neuroinflammation; RNS; ROS
    DOI:  https://doi.org/10.1007/s10787-026-02169-7
  7. Neurosci Biobehav Rev. 2026 Mar 18. pii: S0149-7634(26)00104-1. [Epub ahead of print] 106647
      Mental disorders are associated with signs of accelerated aging across diagnostic groups, both at the population, organ, cellular, and molecular level. Oxidative stress-induced damage to nucleic acids (DNA and RNA) (NA-OXS) is a well-established molecular aging mechanism that is implicated in aging per se as well as in a wide range of age-related disorders. Here, we review the work by us and others on the role of NA-OXS in mental disorders. NA-OXS is increased across diagnostic groups, with the highest levels found in disorders that are also linked to the highest mortality, such as dementias, schizophrenia, and bipolar disorder. NA-OXS appears to be mostly a trait phenomenon that may be sensitive to symptom changes in some disorders. NA-OXS on RNA predict mortality in the general population, patients with type-2 diabetes, and people with mental disorders. Finally, NA-OXS may be reduced by antidepressants. We suggest an overarching theory on how mental disorders and associated intermediate factors, such as poor health behaviors, medical care, chronic neurohormonal stress, and medication, may converge on affecting levels of NA-OXS, which in turn is driving accelerated aging and the risk of age-related somatic illness; a mechanism which could potentially be targeted by existing or future interventions.
    Keywords:  Mental disorders; aging; antidepressants; medical morbidity; mortality; nucleic acids; oxidative stress
    DOI:  https://doi.org/10.1016/j.neubiorev.2026.106647
  8. Endocrinol Diabetes Metab. 2026 Mar;9(2): e70182
       BACKGROUND: Several meta-analyses suggest that Coenzyme Q10 (CoQ10) supplementation is associated with glycemic control; however, findings about fasting blood glucose (FBG) and haemoglobin A1c (HbA1c) remain inconsistent across studies. Accordingly, this study aimed to synthesise the results to present a firm conclusion in relation to the efficacy of CoQ10 on glycemic control.
    METHODS: A systematic search was conducted to find meta-analyses of randomised controlled trials using PubMed, Scopus, Web of Science and the Cochrane Database of Systematic Reviews from inception to March 6, 2025. Also, the methodological quality of included studies was evaluated using the AMSTAR2 tool.
    RESULTS: In total, eight meta-analyses were included in this umbrella systematic review and meta-analysis. Pooled analysis using standardized mean difference analysis demonstrated that CoQ10 is associated with decreased FBG. While it didn't exert any significant changes on the HbA1c, HOMA-IR, and insulin levels. In addition, the combined effect of CoQ10 using weighted mean difference analysis revealed that CoQ10 is able to decrease the FBG (5.04 mg/dL), HbA1c (0.17%), HOMA-IR (0.72), and insulin (1.32 μIU/mL) levels significantly.
    CONCLUSION: The present study suggests that CoQ10 supplementation may have a moderate beneficial effect on glycemic control in diabetic patients, though findings differ depending on analytic approach.
    Keywords:  CoQ10; glycemic indices; insulin; randomised controlled trials; type 2 diabetes; umbrella meta‐analysis
    DOI:  https://doi.org/10.1002/edm2.70182
  9. Climacteric. 2026 Mar 16. 1-7
       OBJECTIVE: This study aimed to determine the age of natural menopause in low-weight women and assess how often laboratory evaluation confirms menopausal status.
    METHOD: A retrospective study was conducted of 3000 women aged >50 years with a history of body mass index (BMI) <18.5 kg/m2 seen at a large academic health system between 2004 and 2020. Women with indeterminate menopausal age, BMI ≥18.5 kg/m2 at menopause or known causes of early menopause were excluded. Clinical data including menopausal age, BMI, reproductive history and laboratory tests were extracted. Associations between BMI and menopausal age were analyzed using unadjusted and multivariable analyses controlling for tobacco use and age at menarche.
    RESULTS: Among 239 women included, menopausal age correlated with BMI (ρ = 0.13, p = 0.04) and remained significant in multivariate regression (p = 0.02). Median menopausal age was 51 years for BMI 18.0 to <18.5 kg/m2, 50 years for BMI 17.0 to <18.0 kg/m2, 50 years for BMI 16.0 to <17.0 kg/m2 and 48 years for BMI <16.0 kg/m2. Only 30 women (12.6%) had postmenopausal follicle stimulating hormone (FSH) measured; 30% were below the diagnostic threshold for menopause (<25 mIU/ml).
    CONCLUSION: Low BMI is associated with earlier menopause. The substantial proportion of low-weight women classified as menopausal who had non-diagnostic FSH levels highlights the complexity of determining menopausal status and the potential for misclassification in this population.
    Keywords:  Menopause; age at natural menopause; estrogen deficiency; hormone testing; low body weight; reproductive aging
    DOI:  https://doi.org/10.1080/13697137.2026.2631509
  10. Geroscience. 2026 Mar 16.
      Cognitive decline during aging may be influenced by peripheral factors, including neuroproteins and pro-inflammatory cytokine levels, body composition, and physical fitness. However, the specific associations between these factors and cognitive performance remain underexplored. A comprehensive assessment, including peripheral cytokine and neuroprotein levels, body composition, physical performance (aerobic fitness and muscle strength), and cognitive function was performed in a cohort of 87 older adults (mean age: 69.3 ± 3.4 years; 66 females, 21 males) with a range of Body Mass Index (BMI): 19.7 - 41.9. Elevated blood levels of N-lactoyl-phenylalanine (Lac-Phe) were associated with better psychomotor speed (r = -0.223, p = 0.034) as measured by the Trial Making Test-A (TMT-A). Elevated pro-inflammatory cytokines (IL-6, IL-8, IL-12) and peripheral clusterin concentrations were also associated with poorer cognitive performance. No significant associations were found between cortisol, brain-derived neurotrophic factor and cognitive function. BMI and fat mass (kg) were positively associated with performance on the Stroop Test, suggesting a negative effect of increased adipose tissue on inhibitory control. In contrast, greater skeletal muscle mass was positively associated with better memory. Physical fitness parameters, including VO2peak, handgrip strength, and maximal aerobic power, showed positive associations primarily with short-term visuospatial memory. Findings from our study suggest that higher levels of adiposity and inflammation are detrimental to cognitive health. Cardiorespiratory fitness, skeletal muscle mass and strength show a protective role, particularly for memory performance in older adults. Future studies assessing the impact of aging and associated cognitive decline should include Lac-Phe and clusterin as biomarkers associated with specific cognitive domains.
    Keywords:  Aging; Cardiorespiratory fitness; Cognition; Cytokines; Neuroproteins
    DOI:  https://doi.org/10.1007/s11357-026-02129-7
  11. Transl Res. 2026 Mar 14. pii: S1931-5244(26)00062-9. [Epub ahead of print]
      Mitochondria are fundamental organelles that regulate cellular homeostasis through energy production, metabolic integration, and signaling cascades. Beyond their bioenergetic role, mitochondrial dysfunction is increasingly recognized as a pivotal instigator of PANoptosis, a novel, coordinated inflammatory cell death pathway that amalgamates key features of pyroptosis, apoptosis, and necroptosis. This integrated cell death is executed by multiprotein complexes termed PANoptosomes, which are nucleated by specific sensors like ZBP1, AIM2, and NLRC5. Central to this process is the release of mitochondrial danger signals, including reactive oxygen species (ROS) and mitochondrial DNA (mtDNA), which act as potent upstream triggers. For instance, ROS can directly oxidize and activate necroptotic mediators like RIPK1, while cytosolic mtDNA engages innate immune sensors such as cGAS-STING and inflammasomes, thereby initiating PANoptosome assembly. Concurrently, defects in core mitochondrial processes including impaired oxidative phosphorylation, disrupted dynamics (fission/fusion), and faulty mitophagy exacerbate these inflammatory signals, creating a permissive environment for PANoptosis. This mitochondrial-PANoptosis axis is implicated in the pathogenesis of a broad spectrum of diseases. Consequently, therapeutic strategies targeting mitochondrial integrity or specific PANoptotic components hold significant promise for mitigating pathological inflammation and cell loss. This review focuses on the molecular mechanisms linking mitochondrial dysfunction to PANoptosis and explores the translational potential of this interplay to reshape therapeutic approaches in diseases.
    Keywords:  & Mitochondrial dysfunction; Cell death; Immune; PANoptosis; Therapeutics
    DOI:  https://doi.org/10.1016/j.trsl.2026.03.004
  12. Food Funct. 2026 Mar 18.
      Background: Olive leaves, a by-product of olive oil production, are rich in oleuropein, a phenolic compound with antioxidant and anti-inflammatory properties. This systematic review and meta-analysis evaluated the effects of olive leaf extract (OLE) and oleuropein on glucose metabolism, lipid profile, and inflammatory markers in randomized clinical trials (RCTs). Methods: The review was registered in PROSPERO (CRD42022375615) and followed PRISMA guidelines. PubMed, Scopus, and Web of Science were searched up to January 2026 without restrictions. Eligible studies were RCTs in adults comparing OLE or oleuropein with placebo or control. Study selection, data extraction, and risk of bias (RoB 2) assessment were performed independently by two reviewers; certainty of evidence was assessed with GRADE. Meta-analyses were conducted when at least two trials reported suitable data. Results: Eleven RCTs were included (4 short-term and 7 continuous-intake trials). Short-term crossover studies showed heterogeneous effects on postprandial glycemia and insulin responses, precluding meta-analysis. In continuous-intake, parallel-design RCTs, pooled analyses demonstrated no statistically or clinically significant effects on glycemic or lipid outcomes. Evidence regarding inflammatory markers was scarce and of low certainty. Conclusion: The current evidence does not support clinical recommendations for OLE supplementation aimed at health benefits. Data from methodologically consistent and robust trials indicate no statistically significant metabolic or inflammatory effects under usual human consumption patterns, whereas evidence from other study designs remains inconclusive. Further high-quality RCTs are required to clarify potential metabolic benefits.
    DOI:  https://doi.org/10.1039/d5fo04235f
  13. Exp Gerontol. 2026 Mar 16. pii: S0531-5565(26)00078-1. [Epub ahead of print] 113100
      Exercise training improves the age-induced decline in oxidative metabolic capacity in cardiac mitochondria. Nuclear respiratory factor-1 (NRF-1) signaling via peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) regulates genes encoding mitochondrial oxidative metabolic enzymes. However, the effects of aging and subsequent exercise training on fatty acid (FA) metabolism-related gene expression via the myocardial PGC-1α-NRF-1 pathway, and the relevance of these changes to improvements in myocardial FA metabolic function, remain unclear. In this study, we examined the hearts of the following male Wistar rats: young sedentary rats (Young-CON; 4 months old), aged sedentary rats (Aged-CON; 23 months old), and aged swim-trained rats (Aged-Ex; 23 months old, trained for 8 weeks, 5 days/week, 90 min/day). Myocardial PGC-1α mRNA and protein levels; myocardial NRF-1 mRNA expression levels; NRF-1 DNA-binding activity to promoter regions of mitochondrial oxidative-metabolism-related genes; and mRNA expression levels of cytochrome c oxidase and cytochrome c, which are NRF-1 target genes, were significantly lower in the Aged-CON group than in the Young-CON group, and significantly higher in the Aged-Ex group than in the Aged-CON group. Furthermore, myocardial enzyme activities associated with mitochondrial oxidative metabolism and ATP concentration were significantly lower in the Aged-CON group than in the Young-CON group and significantly higher in the Aged-Ex group than in the Aged-CON group. These findings suggest that transcriptional regulation via the PGC-1α-NRF-1 pathway may contribute to the age-related decline in mitochondrial energy metabolism and may mediate its restoration by exercise training in the heart.
    Keywords:  Aging; Endurance exercise training; Myocardial mitochondrial oxidative metabolism; Nuclear respiratory factor-1; Peroxisome proliferator-activated receptor γ coactivator-1α
    DOI:  https://doi.org/10.1016/j.exger.2026.113100
  14. Front Pharmacol. 2026 ;17 1723748
      Mitochondria act as a central integrative hub for oxidative phosphorylation, calcium homeostasis and metabolic signaling, reflecting their evolutionary origin from an α-proteobacterial endosymbiont. Although nearly 90% of their ancestral genes have been transferred to the nuclear genome, their role extends far beyond energy production. Emerging evidence positions mitochondria as active modulators of stress responses, which we term the "Mito-Mood Hypothesis." This framework proposes that mitochondrial dynamics actively regulate gene expression and signaling, thereby shaping vulnerability to mood disorders such as depression, dysthymia, and seasonal affective disorder. Consistent with this view, patients with major depressive disorders show altered expression of nuclear-encoded mitochondrial genes, linking bioenergetics directly to psychiatric risk. We further discuss how oxidative phosphorylation (OXPHOS) modulates neurotransmitter cycles and how mitohormesis-adaptive responses to mild mitochondrial stress-can enhance resilience and cognition. Beyond psychiatry, mitochondrial vulnerability manifests in clinical settings: patients with mitochondrial diseases face elevated anesthetic risk, where agents such as propofol or volatile anesthetics may precipitate life-threatening metabolic crises. Collectively, these insights underscore mitochondria as central regulators of human health and highlight novel therapeutic opportunities bridging mood disorders and perioperative medicine.
    Keywords:  circadian cycle; mitochondria; mitochondrial dynamics; mood disorders; neurotransmission; oxidative phosphorylation; perioperative stress
    DOI:  https://doi.org/10.3389/fphar.2026.1723748