bims-micpro Biomed News
on Discovery and characterization of microproteins
Issue of 2022‒01‒09
one paper selected by
Thomas Farid Martínez
University of California, Irvine

  1. STAR Protoc. 2022 Mar 18. 3(1): 101037
      Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling, are sophisticated and expensive. Here, we present an easy and cost-effective protocol to screen for potential micropeptide-encoding lncRNAs by polysome profiling in suspension cell lines. When combined with quantitative PCR, this protocol facilitates the identification of a number of translating lncRNAs simultaneously. For complete details on the use and execution of this protocol, please refer to Sun et al. (2021).
    Keywords:  Cell separation/fractionation; Molecular Biology