bims-micpro Biomed News
on Discovery and characterization of microproteins
Issue of 2021‒07‒25
one paper selected by
Thomas Martinez
Salk Institute for Biological Studies

  1. J Extracell Vesicles. 2021 Jul;10(9): e12123
      Advancements in omics-based technologies over the past few years have led to the discovery of numerous biologically relevant peptides encoded by small open reading frames (smORFs) embedded in long noncoding RNA (lncRNA) transcripts (referred to as microproteins here) in a variety of species. However, the mechanisms and modes of action that underlie the roles of microproteins have yet to be fully characterized. Herein, we provide the first experimental evidence of abundant microproteins in extracellular vesicles (EVs) derived from glioma cancer cells, indicating that the EV-mediated transfer of microproteins may represent a novel mechanism for intercellular communication. Intriguingly, when examining human plasma, 48, 11 and 3 microproteins were identified from purified EVs, whole plasma and EV-free plasma, respectively, suggesting that circulating microproteins are primarily enriched in EVs. Most importantly, the preliminary data showed that the expression profile of EV microproteins in glioma patient diverged from the health donors, suggesting that the circulating microproteins in EVs might have potential diagnostic application in identifying patients with glioma.
    Keywords:  cancer; extracellular vesicles (EVs); lncRNA‐encoded microproteins; long noncoding RNA (lncRNA); small open reading frames (smORFs)