bims-micpro Biomed News
on Discovery and characterization of microproteins
Issue of 2021‒05‒16
one paper selected by
Thomas Martinez
Salk Institute for Biological Studies

  1. Bioinformatics. 2021 May 10. pii: btab339. [Epub ahead of print]
      MOTIVATION: The annotation of small open reading frames (smORFs) of less than 100 codons (<300 nucleotides) is challenging due to the large number of such sequences in the genome.RESULTS: In this study, we developed a computational pipeline, which we have named ORFLine, that stringently identifies smORFs and classifies them according to their position within transcripts. We identified a total of 5744 unique smORFs in datasets from mouse B and T lymphocytes and systematically characterized them using ORFLine. We further searched smORFs for the presence of a signal peptide, which predicted known secreted chemokines as well as novel micropeptides. Four novel micropeptides show evidence of secretion and are therefore candidate mediators of immunoregulatory functions.
    AVAILABILITY: Freely available on the web at
    SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.