bims-mesote Biomed News
on Mesothelioma
Issue of 2026–01–11
seven papers selected by
Laura Mannarino, Humanitas Research



  1. J Clin Med. 2025 Dec 20. pii: 42. [Epub ahead of print]15(1):
      Background: The heritage of occupational and environmental asbestos exposure in Piedmont, Italy, has resulted in an enduring diffuse pleural mesothelioma (DPM) epidemic. Our study aimed to investigate the accuracy of Pleural biopsy (PB) via thoracoscopy (or video-assisted thoracic surgery-VATS) and analyze the diagnostic path of patients who experienced an initial DPM misdiagnosis. Methods: Patients who underwent PB by VATS for suspected DPM from 2004 to 2013 were analyzed. The Registry of Malignant Mesothelioma (RMM) records were examined to cross-check incident cases and identify misdiagnosed DPM. The sensitivity and specificity of the initial PB assessment versus the final classification of cases by RMM were evaluated. Results: Data from 552 patients were analyzed, and DPM was diagnosed in 178 cases (32%). Sensitivity and specificity were 93% and 100%, respectively. The number of false-negative PBs was 14 (2%). Of those, 10 (71%) had an initial diagnosis of chronic pleuritis, 3 (28.5%) were initially classified as mesothelial proliferation, and 1 had reactive mesothelial proliferation. All of them reported a history of asbestos exposure, and the correct diagnosis was reached after a median of 160 days. One- and four-year survival rates were 52% and 10% in DPM PB-positive cases and 50% and 19% in false-negative cases. Conclusions: When a strong clinical suspicion after a negative PB remains, iterative biopsy attempts should be considered, especially if a history of asbestos exposure is reported. In high-volume centers, the DPM misdiagnosis rate remains low, and future advancements in diagnostic technologies could further increase the accuracy and efficacy of histologic diagnosis.
    Keywords:  asbestos; diagnosis; diffuse mesothelioma; malignant pleural mesothelioma; pleural effusion; thoracoscopy
    DOI:  https://doi.org/10.3390/jcm15010042
  2. Lung Cancer. 2026 Jan 03. pii: S0169-5002(25)00785-8. [Epub ahead of print]212 108893
       OBJECTIVES: Pleural mesothelioma (PM) is an aggressive thoracic cancer related to historical exposure to asbestos fibres. Symptoms often appear at an advanced stage, leading to delayed diagnosis and dismal prognosis. Early diagnosis is thus crucial in improving patient outcome. Current biomarker research for early detection focuses on different -omics research fields (genomics, proteomics, transcriptomics, metabolomics and volatomics), however with no clinically useful result. Moreover, currently no screening program is advocated for asymptomatic individuals with an established asbestos exposure. The aim of this review is to summarise the advances in different -omics fields and to pinpoint state-of-the art biomarkers with the highest potential to serve as primary targets in clinical trials for early PM detection or screening.
    METHODS: A literature search was performed in the databases MEDLINE and Web Of Science. Research articles published before 1 August 2025 were eligible.
    RESULTS: In total, 63 articles were included in this review, with specific focus on radiomics, genomics, transcriptomics, epigenomics, proteomics, metabolomics, and volatomics. Several research groups have focused on investigating biomarkers or screening techniques for pleural mesothelioma among individuals with a history of asbestos exposure. Notable approaches include using low-dose Computed Tomography, and determining mesothelin levels and micro-RNAs in blood, and volatile organic compounds in exhaled breath.
    DISCUSSION: Single biomarkers like miRNAs, mesothelin, and VOCs show promise, but further validation is needed in larger cohorts with correct control groups. A multi-omics approach, which integrates biomarker panels from various -omics areas, has the potential to enhance diagnostic accuracy.
    Keywords:  Biomarkers; Pleural mesothelioma; Screening; −Omics
    DOI:  https://doi.org/10.1016/j.lungcan.2025.108893
  3. Transl Lung Cancer Res. 2025 Dec 31. 14(12): 5465-5478
       Background: Pleural mesothelioma (PM) has a poor prognosis, and immune checkpoint inhibitors (ICIs) have reshaped first-line therapy. However, real-world data comparing first-line immunotherapy with chemotherapy ± targeted therapy and defining optimal second-line strategies in Chinese patients remain limited. This multicenter retrospective real-world study aimed to compare survival outcomes between first-line immunotherapy and chemotherapy-based regimens and to evaluate the effectiveness of different subsequent systemic therapies in patients with PM.
    Methods: This multicenter retrospective real-world cohort included patients with pathologically confirmed PM who received at least one line of systemic therapy at tertiary hospitals in China between January 2015 and January 2025. Patients were grouped by first-line regimen (immunotherapy vs. chemotherapy ± targeted therapy). The primary endpoint was overall survival (OS); secondary endpoints were progression-free survival (PFS), objective response rate (ORR), disease control rate (DCR), and safety. Generalized propensity scores with overlap weighting (OW) were used to balance baseline covariates, followed by weighted Kaplan-Meier and Cox regression analyses. For post-first-line analyses, chemo-start and immuno-start cohorts were used to compare OS across second-line chemotherapy, chemo-immunotherapy, and dual immunotherapy. Hazard ratio (HR), 95% confidence interval (CI), and Eastern Cooperative Oncology Group (ECOG) performance status (PS) were reported.
    Results: Seventy-eight patients were included (chemotherapy ± targeted, n=50; immunotherapy, n=28; median follow-up, 35.7 months). After weighting, first-line immunotherapy improved OS vs. chemotherapy ± targeted therapy (weighted HR, 0.47; 95% CI: 0.23-0.95) and showed a trend toward longer PFS (weighted HR, 0.64; 95% CI: 0.34-1.17). OS benefit was greater in patients with ECOG PS 0-1 (HR, 0.44; 95% CI: 0.21-0.93), non-epithelioid histology (HR, 0.30; 95% CI: 0.10-0.97), or no prior radiotherapy (HR, 0.29; 95% CI: 0.12-0.68). In multivariate models, first-line immunotherapy (HR, 0.34; 95% CI: 0.13-0.90) and prior radiotherapy (HR, 0.35; 95% CI: 0.14-0.86) were independent protective factors for OS. ORR and DCR were similar between groups, and immune-related adverse events occurred in 14/28 (50.0%) immunotherapy patients, mostly grade 1-2, with no immune-related deaths. In the chemo-start cohort, second-line dual immunotherapy improved OS vs. chemo-immunotherapy (HR, 0.13; 95% CI: 0.03-0.62) but not vs. chemotherapy alone (HR, 0.43; 95% CI: 0.17-1.11), and chemo-immunotherapy did not differ from chemotherapy (HR, 2.08; 95% CI: 0.94-4.57). In the smaller immuno-start cohort, no significant OS differences were seen between second-line strategies (weighted Cox P=0.31).
    Conclusions: In this multicenter real-world Chinese cohort, first-line immunotherapy was associated with a clinically meaningful OS improvement in PM, particularly among patients with good PS, non-epithelioid histology, or no prior radiotherapy. Dual immunotherapy may be a promising second-line option after chemotherapy, warranting confirmation in larger prospective studies.
    Keywords:  Pleural mesothelioma (PM); first-line therapy; immune checkpoint inhibitors (ICIs); second-line treatment
    DOI:  https://doi.org/10.21037/tlcr-2025-1-1347
  4. J Nucl Med. 2026 Jan 08. pii: jnumed.125.270906. [Epub ahead of print]
      High expression of fibroblast activation protein (FAP) has been associated with inferior survival in several tumor entities. Novel 68Ga-radiolabeled FAP inhibitors (68Ga-FAPIs) allow noninvasive measurement of FAP, which enables the development of prognostic imaging parameters from 68Ga-FAPI PET/CT. In this study, we compared the prognostic value of 68Ga-FAPI-46 with 18F-FDG PET in a cohort of patients with malignant pleural mesothelioma from the FAPI PET observational trial (NCT04571086). Methods: Between May 2020 and January 2024, 49 patients with suspected or proven malignant mesothelioma were recruited, 39 of whom were eligible for data analysis. All patients underwent 68Ga-FAPI-46 and 18F-FDG PET/CT less than 4 wk apart. Tumor burden was measured semiautomatically, and SUVmax, SUVmean, and volumetric parameters (metabolic tumor volume [MTV], total lesion glycolysis/total lesion fibroblast activation, and total tumor SUV) were calculated. The FAP immunoreactive score (IRS) was calculated for tumor samples from a subset of patients (n = 19). Overall survival and progression-free survival were assessed per revised mRECIST (version 1.1). Survival analyses were performed with univariate and multivariate Cox regression and with Kaplan-Meier curves for clinical and imaging parameters, stratified by median. Results: Univariate analysis showed significant survival differences for all volumetric parameters for 68Ga-FAPI-46 and 18F-FDG (e.g., 68Ga-FAPI-46 MTV, 262 d vs. 737 d; P = 0.008 vs. 18F-FDG MTV, 336 d vs. 760 d; P = 0.012). Multivariate analysis revealed that MTV was an independent prognostic marker for 68Ga-FAPI-46 (hazard ratio, 4.44; 95% CI, 1.20-16.43; P = 0.025) and 18F-FDG (hazard ratio, 7.01; 95% CI, 1.29-38.2; P = 0.024). Kaplan-Meier analysis of the FAP IRS found that a higher IRS was associated with poorer survival (438 d with an IRS of 0-3 vs. 1,076 d with an IRS of 4-12; P = 0.04), but no significant difference was observed in univariate and multivariate analyses. Conclusion: In this modest exploratory cohort of patients with malignant pleural mesothelioma, MTV determined by 68Ga-FAPI-46 and 18F-FDG PET/CT had similar prognostic value, and high MTV was an independent risk factor. 68Ga-FAPI-46 not only complements a diagnostic work-up but also provides prognostic value and could offer alternative theranostic strategies for these patients.
    Keywords:  FAPI; cancer imaging; fibroblast activation protein; mesothelioma; theranostic
    DOI:  https://doi.org/10.2967/jnumed.125.270906
  5. Comput Biol Chem. 2025 Dec 27. pii: S1476-9271(25)00535-3. [Epub ahead of print]121 108871
      This study explores the network pharmacology (NP) and molecular dynamics (MD) simulation analysis of pleural mesothelioma (PM) related enzymes. Through the investigation of 1253 associated genes, culminating in a protein-protein interaction (PPI) network constructed using the STRING database. Pathway analysis identified critical signaling pathways, including MAPK, PI3K/AKT, and RAS, associated with PM pathogenesis. Furthermore, we have synthesized plumbagin-indole-3-proponic acid (PLU-IPA) from plumbagin (PLU) and assessed the toxicity profiles of PLU and PLU-IPA, revealing a reduction in toxicity following IPA incorporation. MD simulations highlighted the stability of PLU-IPA complexes with various proteins (IL6, KRASG12D, SRC and TNFα), supported by analyses of root mean square deviation (RMSD), root mean square fluctuations (RMSF), clustering, and dynamic cross-correlation matrices (DCCM). Principal component analysis (PCA) assessment elucidated the conformational dynamics of the complexes. Additionally, MMGBSA and decomposition binding free energy calculations provided insights into the energetics of ligand binding. Notably, low-frequency mode analyses via Elastic Network Models (ENM) offered a comprehensive view of protein flexibility and ligand interactions. The prominent conformation modifications of each complex during MD simulation has been determined via Markov state model confirms the stability of PLU-IPA in the binding site. These findings underscore the intricate molecular mechanisms underlying PM and highlight PLU-IPA as a potential therapeutic target for future investigations.
    Keywords:  Elastic network model; Markov state model; Molecular docking and molecular dynamics simulation; Network pharmacology; Pleural mesothelioma and Plumbagin-Indole-3-propionic acid ester
    DOI:  https://doi.org/10.1016/j.compbiolchem.2025.108871
  6. Oncogene. 2026 Jan 07.
      Mesothelioma is an aggressive cancer that is often characterized by loss of the BRCA1-associated protein 1 (BAP1) tumor suppressor gene. This alteration typically occurs as an early clonal event in mesothelioma development, making it a promising candidate for both diagnostic and therapeutic applications. Functionally, BAP1 regulates gene expression through interactions with Polycomb-group complexes, and it plays roles in various other cellular processes including DNA repair, replication stress, and cell metabolism. While preclinical research has identified multiple potential vulnerabilities in BAP1-deficient tumors-including sensitivity to EZH2-, HDAC-, PARP-, and FGFR-inhibitors-translating these findings to the clinic remains a challenge. In this review, we provide a comprehensive overview of BAP1's molecular functions in mesothelioma, with a focus on their translation into clinical therapeutics for this hard-to-treat malignancy.
    DOI:  https://doi.org/10.1038/s41388-025-03672-x