bims-meract Biomed News
on Metabolic reprogramming and anti-cancer therapy
Issue of 2026–06–21
eighteen papers selected by
Andrea Morandi, Università degli Studi di Firenze



  1. PeerJ. 2026 ;14 e21400
      In the last few years, metabolic reprogramming has been recognized as a fundamental characteristic of cancer, and is also acknowledged as a crucial cause to drug resistance, which consistently acts as a significant barrier in cancer treatment by allowing tumor cells to adapt and escape various therapies. This review gives a systematically investigation of how metabolic reprogramming contributes to drug resistance in cancer, including aerobic glycolysis (also known as the Warburg effect), lactate metabolism, glutamine addiction, lipid synthesis reprogramming, mitochondrial and ion metabolic changes. Furthermore, by clarifying the mechanisms behind these reprogrammed metabolic pathways, we explain how these changes lead to drug resistance and highlight potential molecular targets for therapeutic intervention. Additionally, we discuss emerging strategies aimed at exploiting these metabolic vulnerabilities, offering new insights for overcoming drug resistance in cancer. By integrating recent discoveries in this field, we present a unified perspective on targeting metabolic vulnerabilities to overcome drug resistance, which is an urgent need in precision oncology, and timely and concise insights for cancer biologists and researchers in the field of exploring the metabolic mechanisms of drug resistance. We hope this review will provide valuable insights for molecular tumor biologists seeking to elucidate the molecular roles of tumor metabolic reprogramming and drug resistance in cancer.
    Keywords:  Cancer; Cancer therapeutics; Drug resistance; Metabolic reprogramming; Tumor microenvironment
    DOI:  https://doi.org/10.7717/peerj.21400
  2. iScience. 2026 Jun 19. 29(6): 116202
      Chemoresistance in acute myeloid leukemia (AML) is associated with a poor prognosis in patients. Ferroptosis is one of the mechanisms by which anthracycline exerts anti-leukemic effects, with drug-resistant AML cells exhibiting increased resistance to ferroptosis. Elevated expression of aldehyde dehydrogenase 3 family member A2 (ALDH3A2) is correlated with poor prognosis in patients with AML. Patients with chemo-resistant AML exhibit higher levels of ALDH3A2, particularly the V subtype. ALDH3A2 degraded 4-hydroxynonenal and regulated the fatty acid proportion and content of AML cells, protecting AML cells against doxorubicin-induced ferroptosis. Moreover, overexpression of ALDH3A2 changes cell membrane fluidity and reduces drug uptake. Histone deacetylase 2 binds to the ALDH3A2 promoter and regulates its expression. The inhibition of ALDH3A2 or HDAC enhances chemotherapeutic efficacy in AML.
    Keywords:  Cancer; Molecular network; Therapeutics
    DOI:  https://doi.org/10.1016/j.isci.2026.116202
  3. Cancer Res. 2026 Jun 18.
      The development of therapy resistance compromises the long-term efficacy of lenvatinib in advanced hepatocellular carcinoma (HCC), highlighting the need to characterize the molecular drivers of resistance. Through integrated multi-omics analysis combining genome-wide CRISPR screening, transcriptomics, and proteomics, we identified HSPA6 as a critical driver of lenvatinib resistance. HSPA6 was consistently upregulated in resistant cell lines and patient tumors, and high expression correlated with poor treatment response and survival. HSPA6 recruited the deubiquitinase USP9X to stabilize the antioxidant enzyme TXNRD1, thereby suppressing lenvatinib-induced ferroptosis. Furthermore, HSPA6 underwent drug-enhanced liquid-liquid phase separation through its IDR1 domain, facilitating the assembly of biomolecular condensates that reinforce TXNRD1 stability and ferroptosis resistance. Leveraging these insights, canagliflozin, an FDA-approved SGLT2 inhibitor, was repurposed as a direct HSPA6-targeting compound that disrupted this resistance axis, restored ferroptosis sensitivity, and synergized with lenvatinib in patient-derived models. This work unveils a dynamic, condensate-driven mechanism of drug resistance and offers a readily translatable strategy to overcome lenvatinib resistance in HCC.
    DOI:  https://doi.org/10.1158/0008-5472.CAN-26-0314
  4. Nat Commun. 2026 Jun 17.
      Therapeutic strategies targeting cancer metabolism are advancing rapidly. However, perturbing distinct nodes within the same metabolic pathway often yields divergent outcomes. Ferroptosis, a metabolic cell death driven by lipid peroxidation, has garnered attention for potentiating antitumor immunity. Here, we demonstrate that interruption of fatty acid oxidation (FAO) at hydroxyacyl-CoA dehydrogenase (HADHA) node promotes tumoral ferroptosis, whereas targeting upstream enzymes does not. HADHA inhibition causes accumulation of hydroxylated C18 (C18-OH) acylcarnitine to exacerbate mitochondrial lipid peroxidation. In vivo, HADHA ablation or acylcarnitine C18-OH supplementation suppresses tumor growth, enhances antitumor T-cell immunity, and potentiates PD-1 blockade therapy. Clinically, elevated plasma acylcarnitine C18-OH correlates with improved prognosis and immunotherapy response in lung cancer patients. Trimetazidine, an approved anti-ischemic drug and HADHA inhibitor, similarly delays tumor progression and augments immunotherapy. Together, our findings identify HADHA as a ferroptosis regulator and offer a clinically actionable strategy to enhance ferroptosis and immunotherapy through metabolic intervention.
    DOI:  https://doi.org/10.1038/s41467-026-74430-0
  5. Cell Death Dis. 2026 Jun 17.
      Therapy-induced senescence (TIS) in cancer cells can be triggered by radiotherapy, chemotherapy, and certain targeted therapeutics. Here, we demonstrate that a new form of TIS, termed fatty acid synthesis therapy-induced senescence (FASTIS), can be induced by pharmacologically targeting de novo lipogenesis. Cancer cells can evade the anti-proliferative effects of clinically relevant inhibitors of core lipogenic enzymes, such as acetyl-CoA carboxylase (ACC) and fatty acid synthase (FASN), by entering in a senescence-like state. FASTIS cancer cells acquire the classical senescence hallmarks, such as cytomorphological remodeling, increased senescence-associated beta-galatosidase (SA-β-gal) activity, activation of cell cycle arrest markers, and hypersensitivity to IFNγ-induced activation of the immune checkpoint PD-L1. mRNA sequencing reveals an FASTIS-associated transcriptomic profile that overlaps between ACC and FASN inhibitors yet differs significantly from that of other mechanistically diverse TIS inducers, including bleomycin, alisertib, doxorubicin, and palbociclib. The FASTIS-encoding transcriptome is characterized by the activation of cholesterol- and acetyl-CoA-related lipogenic pathways, as well as cell-intrinsic innate immune responses. This profile is characterized as highly senescent (≥0.95) by the machine learning-based senescence predictor SENCAN. Mapping the metabolome and lipidome in FASTIS cells reveals a significant sterol lipid enrichment, including substantial increases in intracellular cholesterol levels. Pharmacological blockade of cholesterol synthesis or promotion of lysosomal cholesterol accumulation, prevents or potentiates the occurrence of SA-β-gal+ FASTIS cells, respectively. Cytokine arrays and miR-146a reporter-based screens revealed that the FASTIS-associated secretory phenotype (FASASP) is highly enriched in immunomodulatory factors but not in inflammatory components. FASTIS cancer cells exhibit an increased overall level of mitochondrial priming, making them highly susceptible to targeted senolysis by BCL-xL-targeting BH3 mimetics and cytokine-activated T cells. The FASTIS phenomenon is a therapeutic outcome through which cancer cells adapt to survive clinical-grade lipogenesis inhibitors. The cholesterol-addicted FASTIS fate can be rationally exploited as a collateral sensitivity in "one-two punch" senogenic-(immuno)senolytic strategies.
    DOI:  https://doi.org/10.1038/s41419-026-08992-8
  6. Cell Death Dis. 2026 Jun 17.
      Chemotherapy resistance and intolerance present significant challenges in the effective treatment of acute myeloid leukemia (AML). However, the role of metabolic reprogramming, particularly lipid metabolic rewiring, in promoting chemotherapy resistance in leukemia has not been fully elucidated. Here, we found that multiple lipid metabolism processes are aberrantly activated in Ara-C resistant AML cells, accompanied by upregulation of JAK-STAT3 signaling and key lipid metabolic regulators, notably SREBP1 and CPT2. Additionally, we discovered W1307, a potent and highly selective STAT3 inhibitor, which demonstrated significant anti-tumor activity both in vitro and in vivo. Genetic and pharmacological inhibition of STAT3 simultaneously suppresses lipid synthesis and catabolism, leading to lipids metabolic disorder accompanied with lipids accumulation, ROS increase, lipid peroxidation and mitochondrial membrane potential decrease. Mechanistically, STAT3 binds to DNA response elements in the promoters of the lipid metabolism associated gene SREBF1 and CPT2, and regulates their expression. Furthermore, inhibition of STAT3 enhances the anti-tumor effect of Ara-C and sensitizes resistant AML cell line to Ara-C through disrupting lipid homeostasis and triggering lipotoxicity. Our findings highlight the critical role of STAT3-driven lipid metabolism reprogramming in chemoresistance. Furthermore, W1307 emerges as a promising therapeutic candidate to overcome chemoresistance in leukemia treatment.
    DOI:  https://doi.org/10.1038/s41419-026-08988-4
  7. Oncogene. 2026 Jun 13.
      Venetoclax-based therapies have revolutionized acute myeloid leukemia (AML) treatment, yet disease progression remains a challenge due to limited response and acquired drug resistance. Identifying molecular drivers of AML progression and resistance is essential for improving therapeutic outcomes. Genes normally silenced in normal tissues but aberrantly activated in cancers, such as Cancer-Testis (CT) genes, are promising targets for cancer diagnostics and therapy. Through a CRISPR screen focused on CT genes and cancer-associated genes exhibiting a CT-like expression profile (CT-like gene), we identified the ATPase TRIP13 as critical for AML progression while dispensable for normal hematopoiesis in genetic mouse models. Mechanistically, we discovered that TRIP13 localizes to mitochondria, where it interacts with apoptosis-inducing factor (AIF), a component of respiratory complex I. This interaction promotes leukemia progression and confers drug resistance by preventing AIF translocation to the nucleus, thereby reducing apoptotic priming and shifting energy metabolism from glycolysis to oxidative phosphorylation (OXPHOS) coupled with increased fatty acid oxidation (FAO). Genetic or pharmacological disruption of the TRIP13-AIF interaction suppressed OXPHOS, reduced leukemia cell viability, and overcame venetoclax resistance in vitro and in vivo. These findings uncover a novel mechanism by which AML cells exploit germline programs to sustain progression and resist therapy, positioning the TRIP13-AIF interaction as a promising therapeutic target for AML.
    DOI:  https://doi.org/10.1038/s41388-026-03852-3
  8. iScience. 2026 Jun 19. 29(6): 116184
      Aggressive-variant prostate cancers (AVPCs) respond poorly to anti-androgen therapy but show sensitivity to taxane-platinum chemotherapy, though outcomes remain poor. We conducted a phase 2 trial testing induction cabazitaxel plus carboplatin (CabCarb) followed by olaparib maintenance versus observation in men with AVPC. The primary endpoint of improved progression-free survival (PFS) was not met, likely due to the study being underpowered after 38.5% of patients experienced early progression (ChemoPD) prior to randomization. No genomic alterations predicted ChemoPD; however, transcriptomic analysis revealed the enrichment of metabolic pathways, including arginine metabolism, in ChemoPD tumors. These findings were supported by metabolomics data from preclinical models. In AVPC models, arginine depletion with ADI-PEG20 enhanced CabCarb efficacy in vitro and in vivo. Together, these results provide insight into the heterogeneity of AVPCs and establish a rationale for novel combination treatment strategies to overcome chemotherapy resistance.
    Keywords:  Health sciences; Medical specialty; Medicine; Oncology
    DOI:  https://doi.org/10.1016/j.isci.2026.116184
  9. Cancer Metab. 2026 Jun 17.
       BACKGROUND: Ovarian cancer (OV) is a leading cause of cancer-related mortality, with cisplatin resistance being a major clinical challenge. This study investigates the role of the Notch ligand DLL1 in mediating ferroptosis resistance and its impact on cisplatin sensitivity in OV.
    METHODS: Multi-omics data, clinical samples, and OV cell lines were used to assess DLL1 expression, its link to prognosis, and its effect on the Nrf2/GPX4 axis. CCK-8, clone formation, and ferroptosis assays evaluated the impact of DLL1 on cell behavior and cisplatin sensitivity.
    RESULTS: DLL1 was upregulated in OV tissues and correlated with poor prognosis. Its knockdown inhibited cell proliferation, migration, and EMT, while inducing ferroptosis, evidenced by increased lipid peroxidation and mitochondrial dysfunction. Mechanistically, DLL1 activated the Nrf2/GPX4 antioxidant axis. DLL1 depletion sensitized OV cells to cisplatin. In vivo, combining DLL1-targeted therapy with a ferroptosis inducer significantly reduced tumor growth in cisplatin-resistant models.
    CONCLUSION: DLL1 drives cisplatin resistance in OV by enhancing ferroptosis resistance via the Notch-Nrf2/GPX4 axis. Targeting DLL1 alongside ferroptosis induction represents a promising therapeutic strategy, positioning DLL1 as a potential biomarker and target in drug-resistant OV.
    Keywords:  Cisplatin resistance; Delta-like ligand 1 (DLL1); Ferroptosis resistance; Notch signaling pathway; Ovarian cancer
    DOI:  https://doi.org/10.1186/s40170-026-00444-3
  10. Transl Lung Cancer Res. 2026 May 31. 15(5): 150
      Tumor metabolic reprogramming is a pivotal mechanism driving acquired resistance to programmed cell death protein 1 (PD-1)/programmed death-ligand 1 (PD-L1) blockade therapy. Under therapeutic pressure, tumor cells undergo extensive metabolic rewiring, encompassing enhanced glycolysis, altered amino acid metabolism, and reprogrammed lipid utilization. This metabolic plasticity intensifies nutrient competition within the tumor microenvironment (TME), leading to the accumulation of immunosuppressive metabolites such as lactate and kynurenine. These metabolites collectively impair effector T cell activation, proliferation, and cytotoxicity, while simultaneously facilitating the expansion and suppressive activity of regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs). In parallel, T cells often exhibit metabolic exhaustion, characterized by mitochondrial dysfunction, reduced oxidative phosphorylation, and impaired metabolic flexibility, which ultimately limits their persistence and anti-tumor efficacy despite checkpoint blockade. Moreover, the intrinsic heterogeneity and adaptability of tumor metabolism promote the selection of resistant subclones during immunotherapy, further undermining treatment durability. To overcome these barriers, emerging combinatorial strategies are focusing on integrating metabolic inhibitors, such as lactate dehydrogenase A (LDHA) and IDO1 inhibitors, with immune checkpoint blockade, or on metabolically engineering T cells to enhance their fitness. Future efforts should emphasize precise patient stratification, development of highly selective metabolic modulators, and rational design of combination therapies to improve both the efficacy and long-term durability of cancer immunotherapy.
    Keywords:  Programmed cell death protein 1/programmed death-ligand 1 blockade (PD-1/PD-L1 blockade); immune therapy resistance; metabolic heterogeneity; metabolic reprogramming
    DOI:  https://doi.org/10.21037/tlcr-2026-1-0171
  11. J Exp Clin Cancer Res. 2026 Jun 17.
      Lipid desaturation is a fundamental biochemical process essential for maintaining membrane fluidity, energy storage, and cellular signaling. It is increasingly recognized that this homeostatic network is frequently dysregulated by malignant cells to support proliferation, evade programmed cell death, and facilitate immune evasion. There are two primary lipid desaturation pathways: the conversion of saturated fatty acids (SFAs) to monounsaturated fatty acids (MUFAs) by stearoyl-CoA desaturase 1 (SCD1), and the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs) via the fatty acid desaturases (FADS). This review explores how tumors utilize the SCD1 axis to mitigate lipotoxic endoplasmic reticulum (ER) stress and ferroptosis. Furthermore, we discuss how the FADS axis presents a distinct metabolic paradox: while it promotes oncogenic signaling and structural plasticity, it concurrently creates an actionable vulnerability to ferroptosis by enriching membranes with peroxidation-prone PUFAs. This metabolic rewiring provides a strong biological rationale for precision therapeutics.We trace the clinical development of desaturase inhibitors, highlighting the recent entry of SCD1 inhibitor, MTI-301, in a Phase 1 clinical trial for solid tumors and the potential repurposing of Aramchol, while detailing how FADS2 plasticity (the "sapienic shunt") drives therapeutic resistance. By integrating these insights into desaturation lipidomics, metabolic modulation via diet-drug interactions, synergistic combination regimens, and stimuli-responsive nanomedicine, we highlight the translational potential of targeting lipid desaturation to overcome metabolic plasticity and treatment resistance in aggressive malignancies.
    Keywords:  Cancer metabolism; ER stress; FADS2; Ferroptosis; Lipid desaturation; MTI-301 (SSI-4); Metabolic plasticity; Precision therapeutics; SCD1; Sapienic shunt
    DOI:  https://doi.org/10.1186/s13046-026-03747-x
  12. Cell Death Dis. 2026 Jun 19.
      Ferroptosis, a regulated form of cell death driven by iron-dependent lipid peroxidation, has recently emerged as a promising therapeutic vulnerability in cancer. Among its key modulators, stearoyl-CoA desaturase 1 (SCD1) plays a pivotal role in ferroptosis resistance by converting saturated fatty acids into monounsaturated fatty acids (MUFAs), thereby limiting the accumulation of highly peroxidizable polyunsaturated fatty acids (PUFAs) and stabilizing membrane integrity under oxidative stress. Multiple oncogenic, transcriptional, epigenetic, and microenvironmental cues enhance tumor survival by promoting SCD1-dependent ferroptosis resistance. Despite this diversity of regulatory inputs, a unifying principle emerges across tumor types: SCD1 activity preserves lipid desaturation as a dominant metabolic strategy to suppress ferroptotic cell death. In this review, we critically analyze how SCD1-dependent ferroptosis resistance is shaped by tumor-specific metabolic states, microenvironmental pressures, and regulatory hierarchies across multiple malignancies. We identify recurring mechanistic themes through which SCD1 integrates redox control, lipid metabolism, stemness, and therapy resistance, while highlighting how these processes are differentially regulated across tissues. Preclinical evidence indicates that targeting SCD1, particularly in rational combination with ferroptosis inducers, chemotherapy, radiotherapy, or immunotherapy, can lower the ferroptotic threshold and overcomes treatment resistance. Finally, we discuss translational challenges and emerging strategies, including tumor-selective delivery, adaptive dosing, and context-specific combinations, that may enable safe and effective exploitation of the SCD1-ferroptosis axis in cancer therapy.
    DOI:  https://doi.org/10.1038/s41419-026-08982-w
  13. Exp Hematol Oncol. 2026 Jun 18. pii: 52. [Epub ahead of print]15(1):
      The emergence of covalent KRASG12C inhibitors has revolutionized the treatment of non-small cell lung cancer (NSCLC), yet acquired resistance remains a major clinical challenge. Here, we systematically identified statins as potent agents capable of overcoming acquired resistance to KRASG12C inhibitors through high-throughput screening of 1,971 FDA-approved compounds. Statin treatment preferentially induced cell death in resistant cells, markedly impairing tumor growth both in vitro and in vivo. Proteomic profiling and pathway analysis revealed upregulation of the mevalonate (MVA) pathway in resistant cells. Genetic silencing of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) and pharmacologic inhibition of the MVA-geranylgeranyl pyrophosphate (GGPP) branch recapitulated the effects of statins. Mechanistically, MVA-GGPP signaling promoted Yes-associated protein (YAP) activation, whereas disruption of this axis by statins or geranylgeranyl transferase I (GGTase-I) inhibitors impaired YAP-driven transcription and cell survival. Combination therapy with simvastatin and KRASG12C inhibitors delayed resistance onset and enhanced antitumor efficacy across multiple in vivo models, with acceptable tolerability. These findings identify the MVA-GGPP-YAP pathway as a therapeutic vulnerability in acquired KRASG12C inhibitor resistance and support repurposing statins to improve KRASG12C-targeted therapy.
    Keywords:  Acquired resistance; KRASG12C inhibitor; Mevalonate pathway; Non-small cell lung cancer; Statin
    DOI:  https://doi.org/10.1186/s40164-026-00797-x
  14. Int Immunopharmacol. 2026 Jun 13. pii: S1567-5769(26)00852-0. [Epub ahead of print]185 117006
       OBJECTIVE: To investigate the molecular mechanisms of lipid metabolic reprogramming promoting tyrosine kinase inhibitors (TKIs) resistance in renal cell carcinoma (RCC).
    METHODS: To establish a sunitinib resistant renal cell carcinoma cell line (786O-R) and screen out arachidonic acid 5-lipoxygenase (ALOX5), a key gene regulating lipid metabolism, by transcriptome sequencing. To explore the expression of ALOX5 in TKI-resistant RCC tissues and cells, and the relationship between ALOX5 expression and the prognosis of RCC patients through databases and clinical samples. To explore the effect of ALOX5 on the malignant biological behavior of RCC cells and drug resistance to sunitinib in vitro. Transcriptome sequencing and molecular experiments were performed to explore the molecular mechanism of ALOX5 regulating lipid metabolism. The effect of ALOX5 inhibition on the tumorigenesis of 786O-R cell was investigated by animal experiments.
    RESULTS: ALOX5 is upregulated in TKI-resistant RCC tissues and cells. High ALOX5 expression is associated with poor prognosis of patients with RCC. Overexpression of ALOX5 can enhance the proliferation, migration, invasion and sunitinib resistance of 786O-R cell. ALOX5 promotes sunitinib resistance in 786O-R cell by regulating lipid metabolism through the p38/ERK/PPARα/CPT1A pathway. Inhibition of ALOX5 inhibited the growth of subcutaneous tumors and lung metastases in 786O-R cell.
    CONCLUSION: ALOX5 mediates sunitinib resistance in RCC by promoting lipid metabolism through the p38/ERK/PPARα/CPT1A pathway. Inhibition of ALOX5 may be a potential therapeutic target for intervention of metabolic abnormalities and overcoming resistance to targeted therapy in RCC.
    Keywords:  ALOX5; Fatty acid oxidation; Lipid metabolic reprogramming; Renal cell carcinoma; Sunitinib resistance
    DOI:  https://doi.org/10.1016/j.intimp.2026.117006
  15. Nat Commun. 2026 Jun 16.
      Metabolic reprogramming is a hallmark of cancer, and the field has predominantly focused on investigating metabolic alterations in tumour cells. However, the relevance, mechanism and consequences of metabolic adaptations in stromal cells remain understudied. Here, we identify aspartoacylase (ASPA) as a metabolic enzyme consistently repressed in tumour stroma and cancer-associated fibroblasts (CAFs). Importantly, we report a reciprocal crosstalk between ASPA and Transforming Growth Factor Beta (TGFβ) signalling that influences fibroblast behaviour. TGFβ suppresses ASPA expression in fibroblasts, whereas ASPA restrains TGFβ-dependent myofibroblast conversion, extracelullar cell matrix (ECM) remodelling, angiogenesis and pro-tumoral macrophage phenotypes. Analyses of human specimens revealed a strong negative prognostic value for ASPA in different tumour types, associated with TGFβ signalling levels and the generation of aggressive pro-tumoral responses. Our findings unveil ASPA expression in fibroblasts as a gatekeeper of TGFβ responses and activation in cancer progression.
    DOI:  https://doi.org/10.1038/s41467-026-73002-6
  16. Front Immunol. 2026 ;17 1836534
      Metabolic reprogramming is a hallmark of cancer, with excessive lactate accumulation driven by aerobic glycolysis profoundly reshaping the tumor microenvironment (TME). Beyond being a metabolic by-product, lactate acts as a signaling metabolite and epigenetic regulator that promotes immune suppression and therapeutic resistance. Lactate-induced acidification impairs immune cell function and reprograms macrophages, T cells, and dendritic cells toward immunosuppressive phenotypes. Moreover, lactate drives lysine lactylation of histone and non-histone proteins, linking metabolic status to transcriptional regulation and oncogenic signaling. Emerging evidence indicates that lactate and lactylation cooperatively enhance tumor survival, invasion, and resistance to chemotherapy, radiotherapy, anti-angiogenic therapy, and immunotherapy. This review summarizes mechanisms of lactate production, transport, and signaling, and discusses therapeutic strategies targeting lactate metabolism, lactylation, and TME acidification, highlighting their potential in precision and combination cancer therapy.
    Keywords:  drug resistance; immune suppression; lactate; lactylation; tumor microenvironment
    DOI:  https://doi.org/10.3389/fimmu.2026.1836534
  17. Cell Death Dis. 2026 Jun 17.
      Chemoresistance remains a major unmet challenge in the clinical management of ovarian cancer. As a metabolism-associated malignancy, the poor prognosis and frequent chemoresistance of ovarian cancer are closely linked to metabolic reprogramming. In this study, we identify estrogen-related receptor α (ERRα) as a key regulator of metabolic plasticity and chemoresistance in ovarian cancer. Bioinformatic analyses of pan-cancer datasets and chemoresistant ovarian cancer samples reveal that high expression of ERRα and the glycolytic rate-limiting enzyme lactate dehydrogenase A (LDHA) is associated with poor clinical outcomes. Elevated serum LDH levels in chemoresistant patients further underscore the importance of metabolic reprogramming in the development of chemoresistance. Mechanistically, ChIP-seq, dual-luciferase reporter assays, and enzymatic colorimetric assays demonstrate that ERRα directly binds to the LDHA promoter region (5'-AGAAGGTCG-3'), activating its transcription and enhancing glycolysis and the production of its end-product, lactate. Scanning electron microscopy, immunofluorescence, Western Blot, and other molecular functional assays show that the ERRα/LDHA axis drives lactate accumulation, downregulates inflammasome-related proteins (NLRP3, caspase-1, GSDMD and GSDMD-N), thereby suppressing pyroptosis and promoting resistance to cisplatin, carboplatin, and paclitaxel in ovarian cancer cells. Pharmacological inhibition of ERRα with XCT790 restores sensitivity to chemotherapeutic agents. In a mouse xenograft model, targeting ERRα enhances the therapeutic efficacy of chemotherapy. Collectively, these findings reveal that the ERRα-LDHA axis increases lactate production, bridging glycolytic metabolism and the suppression of pyroptosis, thereby facilitating chemoresistance in ovarian cancer. Targeting the ERRα/LDHA pathway and developing ERRα inhibitors may represent promising strategies to overcome chemoresistance in ovarian cancer.ERRα targets the promoter region of LDHA, promoting its transcription and the production of the glycolytic product lactate, inhibiting the NLRP3/caspase-1/GSDMD pathway, reducing cell pyroptosis, and helping ovarian cancer cells resist the cytotoxic effects of carboplatin and paclitaxel. Created in BioRender. Ren, Y. (2025) https://BioRender.com/qeh0dw8.
    DOI:  https://doi.org/10.1038/s41419-026-08986-6
  18. Cancer Lett. 2026 Jun 15. pii: S0304-3835(26)00445-3. [Epub ahead of print]656 218681
      Tumor metabolic reprogramming is a hallmark of aggressive head and neck cancer (HNC), with enhanced glycolytic activity representing a major metabolic phenotype. However, how stromal signals in the tumor microenvironment (TME) sustain this process remains unclear. Here, we identify LINC02420 as a long noncoding RNA (lncRNA) enriched in cancer-associated fibroblast-derived extracellular vesicles (CAF-EVs). CAFs delivered LINC02420 to recipient HNC cells through EVs. LINC02420 increased lactate production and cell migration in HNC cells, and enhanced glucose-derived labeling of glycolytic intermediates and lactate. Reducing LINC02420 in donor CAFs attenuated the glycolysis-enhancing and pro-malignant effects of CAF-EVs. Mechanistically, CAF-EV-associated LINC02420 binds AEG-1, encoded by MTDH, and stabilizes AEG-1 protein. Stabilized AEG-1 enhances NF-κB signaling and p65 nuclear translocation. It also supports formation of an AEG-1-p65-p300 transcriptional complex at the HIF1A promoter, thereby increasing HIF1A transcription and glycolysis-related gene expression. In vivo, CAF-derived LINC02420 promoted HNC tumor growth. Clinically, LINC02420 was elevated in HNC tissues and plasma-derived EVs, and high tumor LINC02420 expression was associated with poorer overall survival. These findings identify a CAF-EV-associated lncRNA mechanism linking TME-derived stromal communication to HIF1A-dependent glycolytic regulation and support plasma EV-associated LINC02420 as a potential circulating biomarker for monitoring HNC progression.
    Keywords:  Cancer-associated fibroblasts; Extracellular vesicles; Head and neck cancer; LINC02420; Tumor microenvironment
    DOI:  https://doi.org/10.1016/j.canlet.2026.218681