bims-lymeca Biomed News
on Lysosome metabolism in cancer
Issue of 2023–06–25
two papers selected by
Harilaos Filippakis, University of New England
  1. Visualizing drug-induced lipid accumulation in lysosomes of live cancer cells with stimulated Raman imaging.
  2. A paradigm shift: AMPK negatively regulates ULK1 activity.
  1. Biomed Opt Express. 2023 Jun 01. 14(6): 2551-2564
    Visualizing drug-induced lipid accumulation in lysosomes of live cancer cells with stimulated Raman imaging.
    Yuhao Yuan, Emmanuel O Olawode, L Nathan Tumey, Fake Lu.
      The low pH of the lysosomal compartment often results in sequestration of chemotherapeutic agents that contain positively charged basic functional groups, leading to anti-cancer drug resistance. To visualize drug localization in lysosomes and its influence on lysosomal functions, we synthesize a group of drug-like compounds that contain both a basic functional group and a bisarylbutadiyne (BADY) group as a Raman probe. With quantitative stimulated Raman scattering (SRS) imaging, we validate that the synthesized lysosomotropic (LT) drug analogs show high lysosomal affinity, which can also serve as a photostable lysosome tracker. We find that long-term retention of the LT compounds in lysosomes leads to the increased amount and colocalization of both lipid droplets (LDs) and lysosomes in SKOV3 cells. With hyperspectral SRS imaging, further studies find that the LDs stuck in lysosomes are more saturated than the LDs staying out of the lysosomes, indicating impaired lysosomal lipid metabolism by the LT compounds. These results demonstrate that SRS imaging of the alkyne-based probes is a promising approach to characterizing the lysosomal sequestration of drugs and its influence on cell functions.
    DOI:  https://doi.org/10.1364/BOE.487527
  2. Autophagy. 2023 Jun 20. 1-3
    A paradigm shift: AMPK negatively regulates ULK1 activity.
    Ji-Man Park, Do-Hyung Kim.
      In glucose-starved cells, macroautophagy (hereafter referred to as autophagy) is considered to serve as an energy-generating process contributing to cell survival. AMPK (adenosine monophosphate-activated protein kinase) is the primary cellular energy sensor that is activated during glucose starvation. According to the current paradigm in the field, AMPK promotes autophagy in response to energy deprivation by binding and phosphorylating ULK1 (UNC-51 like kinase 1), the protein kinase responsible for autophagy initiation. However, conflicting findings have been reported casting doubts about the current established model. In our recent study, we have thoroughly reevaluated the role of AMPK in autophagy. Contrary to the current paradigm, our study revealed that AMPK functions as a negative regulator of ULK1 activity. The study has elucidated the underlying mechanism and demonstrated the significance of the negative role in controlling autophagy and maintaining cellular resilience during energy depletion.Abbreviations: AMPK: adenosine monophosphate-activated protein kinase; ULK1: UNC-51 like kinase 1; MTORC1: mechanistic target of rapamycin complex 1; ATG14: autophagy-related protein 14; PIK3C3: phosphatidylinositol 3-kinase catalytic subunit type 3; ATP: adenosine triphosphate; VPS34: vacuolar protein sorting 34; BECN1: Beclin 1; AMPKα: AMPK catalytic subunit α; LKB1: liver kinase B1; PIK3R4: phosphatidylinositol 3-kinase regulatory subunit 4.
    Keywords:  AMPK; LKB1; MTORC1; ULK1; energy stress; glucose starvation
    DOI:  https://doi.org/10.1080/15548627.2023.2223465
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