bims-lycede Biomed News
on Lysosome-dependent cell death
Issue of 2026–03–29
two papers selected by
Sofía Peralta, Universidad Nacional de Cuyo
  1. Repair condensates and lipid domains in lysosome integrity.
  2. Palmitoylation-dependent probes for labeling the Golgi apparatus.
  1. Trends Cell Biol. 2026 Mar 25. pii: S0962-8924(26)00033-4. [Epub ahead of print]
    Repair condensates and lipid domains in lysosome integrity.
    Claudio Bussi, Weiping Li.
      Lysosomes are sophisticated signaling hubs whose function depends on membrane integrity. A breach of this barrier, known as lysosomal membrane permeabilization, triggers inflammation and cell death, driving pathologies from lysosomal storage disorders to neurodegeneration. Cells counter membrane damage with diverse repair mechanisms, including endosomal sorting complexes required for transport machinery, sphingomyelin scrambling, annexin-mediated scaffolding, lipid transport, and stress granule plugging. This diversity suggests singular strategies are insufficient, posing an 'orchestration challenge' regarding precise initiation, spatial organization, and temporal coordination. This opinion article proposes that biomolecular condensation, initiated by damage cues, acts as a primary organizing principle. We suggest lysosomal injury nucleates de novo 'repair condensates' that stabilize compromised membranes and serve as recruitment and organizational hubs for repair machinery.
    Keywords:  biomolecular condensates; lipids; lysophagy; lysosomes; membrane damage
    DOI:  https://doi.org/10.1016/j.tcb.2026.03.002
  2. Methods Enzymol. 2026 ;pii: S0076-6879(26)00005-4. [Epub ahead of print]728 305-325
    Palmitoylation-dependent probes for labeling the Golgi apparatus.
    Shunsuke Sawada, Keita Tsutsui, Shinya Tsukiji.
      Small-molecule fluorescent probes that selectively stain the Golgi apparatus in living cells are valuable tools for investigating Golgi-associated biology and disease. Conventional ceramide-based stains have been widely used, but they require complex staining procedures and often exhibit low Golgi specificity. To address these limitations, we recently developed a series of fluorescent probes incorporating the tri-N-methylated myristoyl-Gly-Cys (myrGC3Me) motif, which targets the Golgi membrane via S-palmitoylation. Modular conjugation of this motif with organic fluorescent dyes yielded blue, green, and red fluorescent probes. Here, we provide detailed procedures for the chemical synthesis and application of these probes to enable simple and rapid staining of the Golgi in living cells with high specificity and negligible cytotoxicity. We also describe procedures for visualizing dynamic changes in Golgi morphology during drug treatments and cell division.
    Keywords:  Fluorescence imaging; Golgi apparatus; S-palmitoylation; Small-molecule fluorescent probes
    DOI:  https://doi.org/10.1016/bs.mie.2026.01.005
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