bims-evecad Biomed News
on Extracellular vesicles and cardiovascular disease
Issue of 2026–04–26
seven papers selected by
Cliff Dominy



  1. Stem Cells. 2026 Apr 24. pii: sxag022. [Epub ahead of print]
      Initially regarded as insignificant cellular waste, extracellular vesicles (EVs) are now recognized as key mediators of intercellular communication, capable of transferring bioactive molecules-such as proteins, nucleic acids, and small compounds-between cells. This function has positioned EVs as promising cell-free therapeutic agents with the potential to transform modern medicine. Stem and progenitor cells naturally release EVs that can replicate many of the therapeutic effects of cell transplantation, while avoiding the challenges associated with administering living cells. EVs derived from various cell sources-including embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells, stromal cells, cardiac progenitor cells, and endothelial progenitor cells-have shown therapeutic efficacy in preclinical models of ischemic heart disease. EVs' translation into human trials in cardiac therapy has lagged behind, however, largely due to challenges related to EV production standardization, regulatory frameworks, and the demonstration of reproducible efficacy in human subjects. Nevertheless, recent milestones have been achieved with the successful completion of the phase I EV-AMI trial (Safety Evaluation of Intracoronary Infusion of EVs in Patients with Acute Myocardial Infarction, NCT04327635) and the enrollment of the first patient in the phase I SECRET-HF trial (Treatment of Non-ischemic Cardiomyopathies by Intravenous Extracellular Vesicles of Cardiovascular Progenitor Cells, NCT05774509). This concise review outlines the evolution of EVs from basic biological discovery to innovative therapeutic platforms, with particular emphasis on their potential applications in acute myocardial infarction. Remaining challenges for clinical translation, including manufacturing and regulatory hurdles, will also be discussed.
    Keywords:  Clinical Studies; Exosomes; Extracellular vesicles; Heart; Myocardial infarction
    DOI:  https://doi.org/10.1093/stmcls/sxag022
  2. NPJ Biomed Innov. 2025 Jul 02. pii: 21. [Epub ahead of print]2(1):
      Cardiac repair following cardiovascular diseases relies heavily on coordinated metabolism across different cells. Exosomes, or small extracellular vesicles secreted by cells, have emerged as pivotal regulators of the metabolic process. This review offers an introductory exploration of the intricate role played by exosomes in cardiac repair and regeneration. Specific metabolism regulation, including metabolism and immune response alterations, is emphasized and analyzed. Additionally, we explore some innovative engineering strategies for improving the therapeutic potential of exosomes in this field. This article aims to provide an integrated framework of comprehension toward exosomes and metabolism in cardiac repair and pave the way for novel therapy designs for cardiovascular diseases by outlining future directions for clinical translation.
    DOI:  https://doi.org/10.1038/s44385-025-00030-0
  3. Cell Commun Signal. 2026 Apr 20.
      
    Keywords:  Cardiac remodelling; Cardiomyocytes; Extracellular vesicles; Fibrosis; Intercellular communication; Ischemia
    DOI:  https://doi.org/10.1186/s12964-026-02866-4
  4. Biosci Rep. 2026 Apr 22. pii: BSR20254075. [Epub ahead of print]46(4):
      Heart failure is a leading cause of morbidity and mortality, highlighting the need for improved therapeutic strategies. Critical to the success of therapies is efficient and targeted delivery systems. Extracellular vesicle-based delivery systems have emerged as promising candidates due to their biocompatibility and low immunogenicity. While extracellular vesicles from a wide variety of cells have been used, they have demonstrated divergent effects on the heart. The present review first summarizes the current sources of extracellular vesicles employed in heart failure therapy and their contrasting outcomes. The review then examines the view that these contrasting outcomes arise from limited cell specificity, inefficient delivery, and suboptimal cargo loading. Finally, the review discusses how these problems are being dealt with by recent advances, including genetic modification, chemical functionalization, and enhanced loading strategies. Together, these approaches highlight the potential of extracellular vesicle-based systems as precision therapeutics in cardiovascular medicine.
    Keywords:  Cardiovascular Disease; Extracellular vesicles; Nano-engineering; RNA-based Therapy; Surface functionalization; Targeted Drug Delivery
    DOI:  https://doi.org/10.1042/BSR20254075
  5. Int J Nanomedicine. 2026 ;21 592274
      Heart failure is a progressive syndrome in which the heart fails to maintain adequate output and remains a leading cause of mortality and healthcare utilization despite guideline-directed pharmacological and device-based therapies. A major contributor to this residual burden is the limited myocardial specificity of conventional treatments, which act systemically and incompletely address the heterogeneous microenvironment of the failing ventricle. Nanomedicine employs nanoscale carriers to reshape pharmacokinetics, protect labile cargo, and enhance delivery to injured myocardium. Preclinical studies in predominantly heart failure with reduced ejection fraction (HFrEF) models, including post-myocardial infarction and pressure-overload injury, demonstrate that targeted nanoplatforms can improve left ventricular ejection fraction by approximately 5-15 percentage points, reduce infarct size by 20-50%, and attenuate fibrosis, inflammation, or cardiomyocyte apoptosis by 30-60% compared with control treatments. Spatially and temporally controlled delivery of small molecules, proteins, and nucleic acids is possible using organic and inorganic nanoparticles and catalytic systems, biomimetic and bioderived carriers, extracellular vesicles, and nanostructured hydrogels or patches, with some also providing mechanical support or theranostic imaging. There is limited evidence in heart failure with preserved ejection fraction (HFpEF). Early studies have focused on inflammation, fibrosis, and microvascular dysfunction rather than on contractile recovery. Importantly, the majority of nanomedicine strategies discussed remain at the preclinical stage, with clinical experience largely confined to early-phase safety and feasibility studies. This review summarizes information from in vitro systems, small- and large-animal models, and newly developed clinical studies, and critically examines translational issues such as toxicity, immunogenicity, scalability, and regulatory complexity. New approaches to cardiac regeneration, such as local delivery of pro-regenerative signals, are also supported by nanomedicine, which facilitates the delivery of pro-regenerative cues, such as regulatory RNAs and extracellular vesicles, to promote cardiomyocyte survival, angiogenesis, and limited myocardial tissue renewal.
    Keywords:  cardiac fibrosis; cardiac regeneration; extracellular vesicles; heart failure; nanomedicine; nanoparticle drug delivery
    DOI:  https://doi.org/10.2147/IJN.S592274
  6. Atheroscler Plus. 2026 Sep;65 100561
       Background: Building on our previous isolation of plaque-derived small extracellular vesicles (sEVs) from carotid atherosclerotic stenosis (CAS) patients, this study aimed to identify plasma sEV-encapsulated miRNAs that reflect plaque vulnerability and predict clinical outcomes, based on differentially expressed miRNAs identified in sEVs from stable versus vulnerable plaques.
    Methods: CAS patients were retrospectively enrolled into four complementary cohorts: (1) a sequencing cohort (n = 12) for miRNA profiling of plaque-derived sEVs; (2) a discovery cohort (n = 62) to identify candidate plasma-derived sEV miRNAs associated with plaque vulnerability; and (3) validation cohort 1 (n = 180; endarterectomy cases) and (4) validation cohort 2 (n = 326; stenting cases) for validating the diagnostic efficacy of the identified plasma-derived sEV miRNAs. Candidate miRNAs were quantified using a High-throughput nano-bio chip integrated system (HNCIB). Their associations with plaque vulnerability and 3-year post-endarterectomy major adverse cardiovascular events (MACE) were analyzed.
    Results: MiRNA sequencing revealed significant upregulation of miR-660-5p in vulnerable plaque-derived sEVs. Its expression levels showed a significant positive correlation between matched plaque- and plasma-derived sEVs from the same patient. Moreover, plasma-derived sEV miR-660-5p levels were independently associated with carotid plaque vulnerability in both validation cohorts, defined either by histopathological criteria (endarterectomy cases) or the clinically validated Plaque-Reporting and Data System (RADS) criteria (stenting cases). Additionally, elevated plasma-derived sEV miR-660-5p levels were also associated with a higher risk of 3-year post-endarterectomy MACE.
    Conclusion: Plasma-derived sEV miR-660-5p represents a promising non-invasive biomarker for assessing carotid plaque vulnerability and predicting 3-year MACE risk following carotid endarterectomy, offering potential for improved risk stratification and therapeutic targeting.
    Keywords:  Carotid atherosclerotic stenosis; Extracellular vesicles; Major adverse cardiovascular event; Plaque vulnerability; microRNA
    DOI:  https://doi.org/10.1016/j.athplu.2026.100561
  7. J Extracell Biol. 2026 Mar;5(3): e70124
      Stroke is the second leading cause of death and a major contributor of long-term disability globally. Ischaemic stroke (IS) is the most common type of stroke; characterised by a blood clot that causes oxygen deprivation in the brain. Survivors often face long-lasting disabilities, imposing significant social and economic burden. Up to a quarter of IS are preceded by a transient ischaemic attack (TIA), where temporary symptoms occur due to a short-term interruption in blood flow. Currently, there is no reliable method to identify which TIA patients are at greatest risk of stroke. Early identification of circulating biomarkers in this cohort could inform clinical follow-up and help prevent future strokes. Extracellular vesicles (EVs) are membrane bound nanoparticles, released by all cell types playing key roles in cell-to-cell communication. EVs have recently emerged as effective biomarkers in disease diagnostics, yet their involvement in IS and TIA remains poorly understood. To explore this, a systematic review was undertaken following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Guidelines. A comprehensive search of PubMed, Scopus and Web of Science up to May 2025 yielded 1206 articles, of which 31 met the inclusion criteria and were analysed. This review highlights the necessity for standardised methodologies, particularly in EV isolation and characterisation, to allow data comparability and clarity of the role of EVs in the pathophysiology of IS and TIA.
    Keywords:  biomarker; extracellular vesicles; ischaemic stroke; transient ischaemic attack
    DOI:  https://doi.org/10.1002/jex2.70124