bims-evecad Biomed News
on Extracellular vesicles and cardiovascular disease
Issue of 2025–10–12
four papers selected by
Cliff Dominy



  1. Brain Behav Immun Health. 2025 Nov;49 101113
      Coronary heart disease (CHD) patients have been found to also possess high anxiety and depression rates, which have been considered as significant risk factors for the disease. One possible underlying biological mechanism behind anxiety/depression being associated with CHD may be exosomes, extracellular vesicles produced by cells throughout the body. These exosomes contain various proteins and miRNAs that could exert a variety of physiological and pathological effects. However, the precise role they play in CHD with anxiety/depression has still not been fully elucidated. In this review, we summarized the current research on exosome involvement in the pathogenesis of CHD with anxiety/depression, particularly focusing on inflammatory responses, neuroendocrine signaling, sympathetic nervous system (SNS) regulation, platelet activation, and endothelial injury. In particular, for inflammatory responses, exosomes have been associated with increased pro-inflammatory cytokine release, such as interleukin (IL)-1β, while for neuroendocrine signaling, the miRNAs miR-135a-5p and miR-320a have been implicated in increasing glucocorticoid signaling. As for SNS regulation, exosome miRNAs are involved in downregulating Nrf2, leading to increased sympathetic nerve excitation, while inhibiting exosome production counteracts platelet activation, in turn lowering thrombosis risk for CHD. Endothelial dysfunction could be promoted by exosomes carrying miR-155. On the other hand, exosome contents exert beneficial effects that could be used for treatment strategies, such as miR-1246 alleviating hypoxia-induced myocardial tissue damage, as well as miR-188-3p lowering nigrostriatal autophagy. Overall, identifying the roles that exosomes play in CHD with concurrent anxiety/depression pathogenesis, as well as potential alleviation, may be greatly beneficial for formulating effective treatment strategies.
    Keywords:  Anxiety; Coronary heart disease; Depression; Exosomes; Pathogenesis; Treatment
    DOI:  https://doi.org/10.1016/j.bbih.2025.101113
  2. Acta Med Indones. 2025 Jul;57(3): 396-402
      Cardiovascular diseases (CVDs) remain a leading cause of morbidity and mortality worldwide. Recent advancements in regenerative medicine have unveiled the promising roles of secretome and exosomes in the treatment of CVDs. In this article, we aim to understand the roles of secretome and exosomes in the cardiovascular system, both in physiological and pathological conditions, and explore the broad applications of secretome and exosomes in mitigating CVD progression. Secretome and exosomes, which play crucial roles in intercellular communication, tissue repair, and immunomodulation, have shown potential in reducing cardiovascular disease progression by inhibiting inflammation, promoting blood vessel growth, and regulating biological mechanisms. Further research is needed to maximize their use in advanced cardiovascular therapy.
    Keywords:  cardiovascular diseases; exosome; secretomes
  3. Clin Proteomics. 2025 Oct 08. 22(1): 36
       BACKGROUND: Saliva-derived small extracellular vesicles (sEVs) are emerging as potential biomarkers for coronary artery disease (CAD). Early identification of these biomarkers is essential for effective management and improved patient outcomes. Our study aimed to isolate and characterize sEVs from saliva to identify non-invasive protein signatures in younger CAD patients.
    METHODS: Saliva sEVs were obtained from 20 CAD patients aged 18-65 years, and 20 healthy controls matched for age and gender. The saliva exosome or sEVs isolation was performed using differential ultracentrifugation and sucrose density gradient methods, and we characterized the sEVs using transmission electron microscopy, scanning electron microscopy, and nanoparticle tracking analysis. Western blotting was done with exosome markers including Anti-Flotilin-1, Anti-TSG-101, and Anti-CD63. Differentially expressed proteins (DEPs) were identified through label-free LC-MS/MS Orbitrap and data was analyzed using Proteome Discoverer 3.0 and statistical analysis using MetaboAnalyst 6.0. Protein-protein interaction network, gene ontology, and pathways enrichment analysis were performed.
    RESULTS: We identified 506 proteins using label-free LC-MS/MS proteomic approaches, with 18 significant DEPs. Notable upregulated proteins included mainly Cystatin-S (CST1/CST2/CST4), Protein S100, alpha-amylase, and Gelsolin (GSN), while downregulated proteins included Serum albumin (ALB) and Apolipoprotein A1 (APOA1). These proteins are linked to inflammation and salivary secretions largely.
    CONCLUSIONS: For the first time, we present unique saliva sEVs protein signatures associated with CAD. Validation in larger cohorts may establish Cystatin S as a potential diagnostic biomarker for CAD.
    Keywords:  Biomarker; Coronary artery disease; Cystatin; Exosome; Saliva; Small extracellular vesicles
    DOI:  https://doi.org/10.1186/s12014-025-09541-9
  4. Front Cell Dev Biol. 2025 ;13 1663973
      Arteriovenous fistula (AVF) stenosis is a complex pathological process caused by venous intimal hyperplasia, and its development is influenced by factors such as surgical injury, hemodynamic changes, inflammatory responses, and cellular proliferation and migration. Exosomes are critical mediators of intercellular communication and carry biomolecules (e.g., deoxyribonucleic acid, ribonucleic acid [RNA], and proteins) that can regulate cell functions and impact inflammatory responses, endothelial cell proliferation, and vascular smooth muscle cell migration. Studies have shown that molecules such as microRNAs within exosomes play significant roles in vascular stenosis-related diseases and can function as potential therapeutic tools and biomarkers for disease diagnosis. In addition, exosomes can serve as drug carriers with good biocompatibility and targeting capabilities, providing new avenues for the diagnosis and treatment of AVF stenosis. This article reviews the application of exosomes in AVF stenosis.
    Keywords:  arteriovenous fistula (AVF); chronic kidney disease (CKD); endothelial-to-mesenchymal transition (EndoMT); exosomes; extracellular vesicles; intimal hyperplasia; vascular remodeling
    DOI:  https://doi.org/10.3389/fcell.2025.1663973