bims-cateng 2023-11-12 papers

bims-cateng

Biomed News

on Cell and tissue engineering

Issue of 2023–11–12
seven papers selected by
Chance Bowman, Dartmouth College

Overcoming biological barriers by virus-like drug particles for drug delivery.
Advances in volumetric bioprinting.
Mass Spectrometry-Based Proteomic Technology and Its Application to Study Skeletal Muscle Cell Biology.
Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.
3D Printed Magneto-Active Microfiber Scaffolds for Remote Stimulation and Guided Organization of 3D In Vitro Skeletal Muscle Models.
Synergistic coupling between 3D bioprinting and vascularization strategies.
Rational design of microRNA-responsive switch for programmable translational control in mammalian cells.

Adv Drug Deliv Rev. 2023 Nov 03. pii: S0169-409X(23)00449-0. [Epub ahead of print] 115134

Overcoming biological barriers by virus-like drug particles for drug delivery.

Yu-Ling Chen, Chun-Jie Bao, Jia-Lun Duan, Ying Xie, Wan-Liang Lu.

  Virus-like particles (VLPs) have natural structural antigens similar to those found in viruses, making them valuable in vaccine immunization. Furthermore, VLPs have demonstrated significant potential in drug delivery, and emerged as promising vectors for transporting chemical drug, genetic drug, peptide/protein, and even nanoparticle drug. With virus-like permeability and strong retention, they can effectively target specific organs, tissues or cells, facilitating efficient intracellular drug release. Further modifications allow VLPs to transfer across various physiological barriers, thus acting the purpose of efficient drug delivery and accurate therapy. This article provides an overview of VLPs, covering their structural classifications, deliverable drugs, potential physiological barriers in drug delivery, strategies for overcoming these barriers, and future prospects.

Keywords:  Virus-like particles (VLPs); drug delivery; physiological barriers; strategies

DOI:  https://doi.org/10.1016/j.addr.2023.115134
Biofabrication. 2023 Nov 03.

Advances in volumetric bioprinting.

Sibo Jing, Liming Lian, Yingying Hou, Zeqing Li, Zihao Zheng, Gang Li, Guosheng Tang, Guoxi Xie, Maobin Xie.

  The three-dimensional (3D) bioprinting technologies are suitable for biomedical applications owing to their ability to manufacture complex and high-precision tissue constructs. However, the slow printing speed of current layer-by-layer (bio)printing modality is the major limitation in biofabrication field. To overcome this issue, volumetric bioprinting (VBP) is developed. VBP changes the layer-wise operation of conventional devices, permitting the creation of geometrically complex, centimeter-scale constructs in tens of seconds. VBP is the next step onward from sequential biofabrication methods, opening new avenues for fast additive manufacturing (AM) in the fields of tissue engineering, regenerative medicine, personalized drug testing, and soft robotics, etc. Therefore, this review introduces the printing principles and hardware designs of VBP-based techniques; then focuses on the recent advances in VBP-based (bio)inks and their biomedical applications. Lastly, the current limitations of VBP are discussed together with future direction of research.

Keywords:  (bio)ink; biofabrication; biomedical application; volumetric bioprinting

DOI:  https://doi.org/10.1088/1758-5090/ad0978
Cells. 2023 Nov 01. pii: 2560. [Epub ahead of print]12(21):

Mass Spectrometry-Based Proteomic Technology and Its Application to Study Skeletal Muscle Cell Biology.

Paul Dowling, Dieter Swandulla, Kay Ohlendieck.

  Voluntary striated muscles are characterized by a highly complex and dynamic proteome that efficiently adapts to changed physiological demands or alters considerably during pathophysiological dysfunction. The skeletal muscle proteome has been extensively studied in relation to myogenesis, fiber type specification, muscle transitions, the effects of physical exercise, disuse atrophy, neuromuscular disorders, muscle co-morbidities and sarcopenia of old age. Since muscle tissue accounts for approximately 40% of body mass in humans, alterations in the skeletal muscle proteome have considerable influence on whole-body physiology. This review outlines the main bioanalytical avenues taken in the proteomic characterization of skeletal muscle tissues, including top-down proteomics focusing on the characterization of intact proteoforms and their post-translational modifications, bottom-up proteomics, which is a peptide-centric method concerned with the large-scale detection of proteins in complex mixtures, and subproteomics that examines the protein composition of distinct subcellular fractions. Mass spectrometric studies over the last two decades have decisively improved our general cell biological understanding of protein diversity and the heterogeneous composition of individual myofibers in skeletal muscles. This detailed proteomic knowledge can now be integrated with findings from other omics-type methodologies to establish a systems biological view of skeletal muscle function.

Keywords:  mass spectrometry; muscle cell biology; muscle proteomics; myology; organelle proteomics

DOI:  https://doi.org/10.3390/cells12212560
Bioact Mater. 2024 Feb;32 98-123

Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.

Aleksandra A Golebiowska, Jonathon T Intravaia, Vinayak M Sathe, Sangamesh G Kumbar, Syam P Nukavarapu.

  Tissue engineering and regenerative medicine have shown potential in the repair and regeneration of tissues and organs via the use of engineered biomaterials and scaffolds. However, current constructs face limitations in replicating the intricate native microenvironment and achieving optimal regenerative capacity and functional recovery. To address these challenges, the utilization of decellularized tissues and cell-derived extracellular matrix (ECM) has emerged as a promising approach. These biocompatible and bioactive biomaterials can be engineered into porous scaffolds and grafts that mimic the structural and compositional aspects of the native tissue or organ microenvironment, both in vitro and in vivo. Bioactive dECM materials provide a unique tissue-specific microenvironment that can regulate and guide cellular processes, thereby enhancing regenerative therapies. In this review, we explore the emerging frontiers of decellularized tissue-derived and cell-derived biomaterials and bio-inks in the field of tissue engineering and regenerative medicine. We discuss the need for further improvements in decellularization methods and techniques to retain structural, biological, and physicochemical characteristics of the dECM products in a way to mimic native tissues and organs. This article underscores the potential of dECM biomaterials to stimulate in situ tissue repair through chemotactic effects for the development of growth factor and cell-free tissue engineering strategies. The article also identifies the challenges and opportunities in developing sterilization and preservation methods applicable for decellularized biomaterials and grafts and their translation into clinical products.

Keywords:  Bio-chemical cues; Chemotactic ability; Clinical therapies; Native micro-environment; Non-immune biomaterials and grafts; Tissue-inks

DOI:  https://doi.org/10.1016/j.bioactmat.2023.09.017
Small. 2023 Nov 10. e2307178

3D Printed Magneto-Active Microfiber Scaffolds for Remote Stimulation and Guided Organization of 3D In Vitro Skeletal Muscle Models.

Gerardo Cedillo-Servin, Ouafa Dahri, João Meneses, Joost van Duijn, Harrison Moon, Fanny Sage, Joana Silva, André Pereira, Fernão D Magalhães, Jos Malda, Niels Geijsen, Artur M Pinto, Miguel Castilho.

  This work reports the rational design and fabrication of magneto-active microfiber meshes with controlled hexagonal microstructures via melt electrowriting (MEW) of a magnetized polycaprolactone-based composite. In situ iron oxide nanoparticle deposition on oxidized graphene yields homogeneously dispersed magnetic particles with sizes above 0.5 µm and low aspect ratio, preventing cellular internalization and toxicity. With these fillers, homogeneous magnetic composites with high magnetic content (up to 20 weight %) are obtained and processed in a solvent-free manner for the first time. MEW of magnetic composites enabled the creation of skeletal muscle-inspired design of hexagonal scaffolds with tunable fiber diameter, reconfigurable modularity, and zonal distribution of magneto-active and nonactive material, with elastic tensile deformability. External magnetic fields below 300 mT are sufficient to trigger out-of-plane reversible deformation. In vitro culture of C2C12 myoblasts on three-dimensional (3D) Matrigel/collagen/MEW scaffolds showed that microfibers guided the formation of 3D myotube architectures, and the presence of magnetic particles does not significantly affect viability or differentiation rates after 8 days. Centimeter-sized skeletal muscle constructs allowed for reversible, continued, and dynamic magneto-mechanical stimulation. Overall, these innovative microfiber scaffolds provide magnetically deformable platforms suitable for dynamic culture of skeletal muscle, offering potential for in vitro disease modeling.

Keywords:  fiber scaffolds; magnetic actuation; melt electrowriting; skeletal muscle; stimuli responsive biomaterials

DOI:  https://doi.org/10.1002/smll.202307178
Biofabrication. 2023 Nov 09.

Synergistic coupling between 3D bioprinting and vascularization strategies.

Miji Yeo, Anwita Sarkar, Yogendra Pratap Singh, Irem Deniz Derman, Pallab Datta, Ibrahim T Ozbolat.

  Three-dimensional (3D) bioprinting offers promising solutions to the complex challenge of vascularization in biofabrication, thereby enhancing the prospects for clinical translation of engineered tissues and organs. While existing reviews have touched upon 3D bioprinting in vascularized tissue contexts, the current review offers a more holistic perspective, encompassing recent technical advancements and spanning the entire multistage bioprinting process, with a particular emphasis on vascularization. The synergy between 3D bioprinting and vascularization strategies is crucial, as 3D bioprinting can enable the creation of personalized, tissue-specific vascular network while the vascularization enhances tissue viability and function. The review starts by providing a comprehensive overview of the entire bioprinting process, spanning from pre-bioprinting stages to post-printing processing, including perfusion and maturation. Next, recent advancements in vascularization strategies that can be seamlessly integrated with bioprinting are discussed. Further, tissue-specific examples illustrating how these vascularization approaches are customized for diverse anatomical tissues towards enhancing clinical relevance are discussed. Finally, the underexplored intraoperative bioprinting was highlighted, which enables the direct reconstruction of tissues within defect sites, stressing on the possible synergy shaped by combining intraoperative bioprinting with vascularization strategies for improved regeneration.

Keywords:  Bioprinting; Intraoperative bioprinting; Tissue and organ substitutes; Vascularization; Vascularized tissues

DOI:  https://doi.org/10.1088/1758-5090/ad0b3f
Nat Commun. 2023 Nov 08. 14(1): 7193

Rational design of microRNA-responsive switch for programmable translational control in mammalian cells.

Hui Ning, Gan Liu, Lei Li, Qiang Liu, Huiya Huang, Zhen Xie.

Artificial RNA translation modulation usually relies on multiple components, such as RNA binding proteins (RBPs) or microRNAs (miRNAs) for off-switches and double-inverter cascades for on-switches. Recently, translational circular RNAs (circRNAs) were developed as promising alternatives for linear messenger RNAs (mRNAs). However, circRNAs still lack straightforward and programmable translation control strategies. Here, we rationally design a programmable miRNA-responsive internal ribosome entry site (IRES) translation activation and repression (PROMITAR) platform capable of implementing miRNA-based translation upregulation and downregulation in a single RNA construct. Based on the PROMITAR platform, we construct logic gates and cell-type classifier circRNAs and successfully identify desired mammalian cell types. We also demonstrate the potential therapeutic application of our platform for targeted cancer cell killing by encoding a cytotoxic protein in our engineered circRNAs. We expect our platform to expand the toolbox for RNA synthetic biology and provide an approach for potential biomedical applications in the future.

DOI: https://doi.org/10.1038/s41467-023-43065-w