bims-biprem Biomed News
on Bioprinting for regenerative medicine
Issue of 2024–09–08
six papers selected by
Seerat Maqsood, University of Teramo
  1. Composite bioink incorporating cell-laden liver decellularized extracellular matrix for bioprinting of scaffolds for bone tissue engineering.
  2. Recent Advances in Implantable 3D-Printed Scaffolds for Repair of Spinal Cord Injury.
  3. 3D printing of bacterial cellulose for potential wound healing applications: Current trends and prospects.
  4. 3D printed biopolymer/black phosphorus nanoscaffolds for bone implants: A review.
  5. Integration of Hydrogels and 3D Bioprinting Technologies for Chronic Wound Healing Management.
  6. Application of 3D reconstruction and 3D printing technology in advanced ovarian cancer surgery: a retrospective study.
  1. Biomater Adv. 2024 Aug 30. pii: S2772-9508(24)00260-7. [Epub ahead of print]165 214017
    Composite bioink incorporating cell-laden liver decellularized extracellular matrix for bioprinting of scaffolds for bone tissue engineering.
    Pengyue You, Hang Sun, Haotian Chen, Changcan Li, Yilei Mao, Tao Zhang, Huayu Yang, Haitao Dong.
      The field of bone tissue engineering (BTE) has witnessed a revolutionary breakthrough with the advent of three-dimensional (3D) bioprinting technology, which is considered an ideal choice for constructing scaffolds for bone regeneration. The key to realizing scaffold biofunctions is the selection and design of an appropriate bioink, and existing bioinks have significant limitations. In this study, a composite bioink based on natural polymers (gelatin and alginate) and liver decellularized extracellular matrix (LdECM) was developed and used to fabricate scaffolds for BTE using 3D bioprinting. Through in vitro studies, the concentration of LdECM incorporated into the bioink was optimized to achieve printability and stability and to improve the proliferation and osteogenic differentiation of loaded rat bone mesenchymal stem cells (rBMSCs). Furthermore, in vivo experiments were conducted using a Sprague Dawley rat model of critical-sized calvarial defects. The proposed rBMSC-laden LdECM-gelatin-alginate scaffold, bioprinted layer-by-layer, was implanted in the rat calvarial defect and the development of new bone growth was studied for four weeks. The findings showed that the proposed bioactive scaffolds facilitated angiogenesis and osteogenesis at the defect site. The findings of this study suggest that the developed rBMSC-laden LdECM-gelatin-alginate bioink has great potential for clinical translation and application in solving bone regeneration problems.
    Keywords:  3D bioprinting; Bioink; Bone tissue engineering; Cavarial defect; Decellularized extracellular matrix
    DOI:  https://doi.org/10.1016/j.bioadv.2024.214017
  2. Adv Pharm Bull. 2024 Jul;14(2): 331-345
    Recent Advances in Implantable 3D-Printed Scaffolds for Repair of Spinal Cord Injury.
    Salar Khaledian, Ghobad Mohammadi, Mohadese Abdoli, Arad Fatahian, Arya Fatahian, Reza Fatahian.
      Spinal cord injury (SCI) is an important factor in sensory and motor disorders that affects thousands of people every year. Currently, despite successes in basic science and clinical research, there are few effective methods in the treatment of chronic and acute spinal cord injuries. In the last decade, the use of 3D printed scaffolds in the treatment of SCI had satisfactory and promising results. By providing a microenvironment around the injury site and in combination with growth factors or cells, 3D printed scaffolds help in axon regeneration as well as neural recovery after SCI. Here, we provide an overview of tissue engineering, 3D printing scaffolds, the different polymers used and their characterization methods. This review highlights the recent encouraging applications of 3D printing scaffolds in developing the novel SCI therapy.
    Keywords:  Collagen; Function recovery; Hydrogel; Neural protection; Polymer-based scaffold; Silk
    DOI:  https://doi.org/10.34172/apb.2024.032
  3. Int J Biol Macromol. 2024 Aug 30. pii: S0141-8130(24)06019-7. [Epub ahead of print]279(Pt 2): 135213
    3D printing of bacterial cellulose for potential wound healing applications: Current trends and prospects.
    Gouripriya D A, Jaideep Adhikari, Poonam Debnath, Shrayana Ghosh, Pooja Ghosh, Sabu Thomas, Emmanuel Ghandilyan, Pavel Gorbatov, Elza Kuchukyan, Seda Gasparyan, Prosenjit Saha.
      Several advances in skin tissue engineering have been made to restore skin damage, facilitating wound healing. Bacterial cellulose (BC), a naturally occurring polymer, has gained attention as a potential material in wound healing due to its unique physical and biological properties. In recent years, with the advent of 3D bio-printing technology, new avenues have opened for fabricating customized wound dressings and scaffolds for tissue engineering purposes. The existing literature in this field mainly focuses on the ways of modifications of bacterial cellulose to make it printable. Still, the applicability of 3D printed scaffolds for wound healing needs to be explored more. This review article focuses on the current research on using 3D-printed BC for skin regeneration, including its production methods and physical and biological properties, making it a better choice than traditional dressings. Furthermore, it also highlights the limitations and future directions for using BC in wound healing and tissue engineering applications. This review provides a comprehensive and up-to-date exploration of the applications of 3D-printed BC in wound healing, drawing insights from pre-existing studies and emphasizing patient compliance, clinical outcomes, and economic viability.
    Keywords:  3D printing; Bacterial cellulose; Tissue regeneration; Wound healing
    DOI:  https://doi.org/10.1016/j.ijbiomac.2024.135213
  4. Int J Biol Macromol. 2024 Aug 30. pii: S0141-8130(24)06033-1. [Epub ahead of print] 135227
    3D printed biopolymer/black phosphorus nanoscaffolds for bone implants: A review.
    Nannan Wu, Jinghong Li, Xinyu Li, Ran Wang, Lexin Zhang, Zhiwei Liu, Tifeng Jiao.
      Bone implantation is one of the recognized and effective means of treating bone defects, but osteoporosis and bone tumor-related bone abnormalities have a series of problems such as susceptibility to infection, difficulty in healing, and poor therapeutic effect, which poses a great challenge to clinical medicine. Three-dimensional things may be printed using 3D printing. Researchers can feed materials through the printer layer by layer to create the desired shape for a 3D structure. It is widely employed in the healing of bone defects, and it is an improved form of additive manufacturing technology with prospective future applications. This review's objective is to provide an overview of the findings reports pertaining to 3D printing biopolymers in recent years, provide an overview of biopolymer materials and their composites with black phosphorus for 3D printing bone implants, and the characterization methods of composite materials are also summarized. In addition, summarizes 3D printing methods based on ink printing and laser printing, pointing out their special features and advantages, and provide a combination strategy of photothermal therapy and bone regeneration materials for black phosphorus-based materials. Finally, the associations between bone implant materials and immune cells, the bio-environment, as well as the 3D printing bone implants prospects are outlined.
    Keywords:  3D printing; Biopolymers; Black phosphorus; Bone tissue; Nanoscaffolds
    DOI:  https://doi.org/10.1016/j.ijbiomac.2024.135227
  5. ACS Biomater Sci Eng. 2024 Sep 04.
    Integration of Hydrogels and 3D Bioprinting Technologies for Chronic Wound Healing Management.
    Moses Kumi, Tianyi Chen, Zhengheng Zhang, An Wang, Gangfeng Li, Zishuo Hou, Tian Cheng, Junjie Wang, Tengjiao Wang, Peng Li.
      The integration of hydrogel-based bioinks with 3D bioprinting technologies presents an innovative approach to chronic wound management, which is particularly challenging to treat because of its multifactorial nature and high risk of complications. Using precise deposition techniques, 3D bioprinting significantly alters traditional wound care paradigms by enabling the fabrication of patient-specific wound dressings that imitate natural tissue properties. Hydrogels are notably beneficial for these applications because of their abundant water content and mechanical properties, which promote cell viability and pathophysiological processes of wound healing, such as re-epithelialization and angiogenesis. This article reviews key 3D printing technologies and their significance in enhancing the structural and functional outcomes of wound-care solutions. Challenges in bioink viscosity, cell viability, and printability are addressed, along with discussions on the cross-linking and mechanical stability of the constructs. The potential of 3D bioprinting to revolutionize chronic wound management rests on its capacity to generate remedies that expedite healing and minimize infection risks. Nevertheless, further studies and clinical trials are necessary to advance these therapies from laboratory to clinical use.
    Keywords:  3D hydrogel bioprinting; chronic wound management; hydrogel bioink; personalized wound dressing
    DOI:  https://doi.org/10.1021/acsbiomaterials.4c00957
  6. Front Oncol. 2024 ;14 1432970
    Application of 3D reconstruction and 3D printing technology in advanced ovarian cancer surgery: a retrospective study.
    Zhihui Cai, Ke Zhang, Linqian Li, Yuping Suo.
       Backgrounds: Advanced ovarian cancer is frequently accompanied by extensive peritoneal metastasis, complicating surgical interventions. This study aims to explore the application of 3D reconstruction and 3D printing technology in the treatment of advanced ovarian cancer.
    Methods: We conducted a retrospective analysis of 60 patients with stage III ovarian cancer who underwent cytoreductive surgery at Hebei University Affiliated Hospital between 2020 and 2023. Patients were randomly assigned to three groups: a 3D visualization group, a 3D visualization plus 3D printing group, and a traditional 2D CT imaging evaluation group. High-precision medical imaging techniques (e.g., CT, MRI) were employed to create digital 3D models, which were then converted into physical entities using 3D printing for surgical planning and simulation.
    Results: Both the 3D visualization group and the 3D visualization plus 3D printing group demonstrated superior outcomes in terms of surgery duration and blood loss compared to the traditional 2D CT group, indicating the efficacy of 3D reconstruction and 3D printing in preoperative planning. Postoperative recovery indicators, such as hospital stay and time to first flatus, were also more favorable in the groups utilizing 3D technology. Although there were no significant differences in postoperative complications and recurrence rates among the three groups, the groups using 3D technology showed advantages in reducing certain complications.
    Conclusions: The results indicate that medical 3D technology has significant value in the surgical planning of advanced ovarian cancer, enhancing surgical precision and reducing intraoperative risks, which may aid in improving postoperative recovery.
    Keywords:  3D printing; 3D visualization; intraoperative risks; ovarian cancer; preoperative planning
    DOI:  https://doi.org/10.3389/fonc.2024.1432970
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