bims-biprem 2024-08-11 papers

bims-biprem

Biomed News

on Bioprinting for regenerative medicine

Issue of 2024–08–11
nine papers selected by
Seerat Maqsood, University of Teramo

3D printing chronicles in medical devices and pharmaceuticals: tracing the evolution and historical milestones.
Clinical applications of 3D printing in colorectal surgery: A systematic review.
Fabrication of GelMA - Agarose Based 3D Bioprinted Photocurable Hydrogel with In Vitro Cytocompatibility and Cells Mirroring Natural Keratocytes for Corneal Stromal Regeneration.
3D-printed titanium scaffolds loaded with gelatin hydrogel containing strontium-doped silver nanoparticles promote osteoblast differentiation and antibacterial activity for bone tissue engineering.
Extrusion-based 3D printing for development of complex capsular systems for advanced drug delivery.
Regenerative cell therapy with 3D bioprinting.
3D printed microneedles: revamping transdermal drug delivery systems.
Research and application discussion of cranial bone model preparation method based on three-dimensional reconstruction and 3D printing technology.
Three-Dimensional Electrically Conductive Scaffolds to Culture Cardiac Progenitor Cells.

J Biomater Sci Polym Ed. 2024 Aug 05. 1-44

3D printing chronicles in medical devices and pharmaceuticals: tracing the evolution and historical milestones.

Riya Patel, Shivani Patel, Nehal Shah, Sakshi Shah, Ilyas Momin, Shreeraj Shah.

  The objective of this study is to collect the significant advancements of 3D printed medical devices in the biomedical area in recent years. Especially related to a range of diseases and the polymers employed in drug administration. To address the existing limitations and constraints associated with the method used for producing 3D printed medical devices, in order to optimize their suitability for degradation. The compilation and use of research papers, reports, and patents that are relevant to the key keywords are employed to improve comprehension. According to this thorough investigation, it can be inferred that the 3D Printing method, specifically Fuse Deposition Modeling (FDM), is the most suitable and convenient approach for preparing medical devices. This study provides an analysis and summary of the development trend of 3D printed implantable medical devices, focusing on the production process, materials specially the polymers, and typical items associated with 3D printing technology. This study offers a comprehensive examination of nanocarrier research and its corresponding discoveries. The FDM method, which is already facing significant challenges in terms of achieving optimal performance and cost reduction, will experience remarkable advantages from this highly valuable technology. The objective of this analysis is to showcase the efficacy and limitations of 3D-printing applications in medical devices through thorough research, highlighting the significant technological advancements it offers. This article provides a comprehensive overview of the most recent research and discoveries on 3D-printed medical devices, offering significant insights into their study.

Keywords:  3D printed medical devise; controlled release; drug targeting; fuse deposition modeling; patent landscape

DOI:  https://doi.org/10.1080/09205063.2024.2386222
Int J Colorectal Dis. 2024 Aug 07. 39(1): 127

Clinical applications of 3D printing in colorectal surgery: A systematic review.

Alyssa C Habermann, William R Timmerman, Stephen M Cohen, Brian W Burkhardt, Michael F Amendola.

   BACKGROUND: The utilization of three-dimensional printing has grown rapidly within the field of surgery over recent years. Within the subspecialty of colorectal surgery, the technology has been used to create personalized anatomical models for preoperative planning, models for surgical training, and occasionally customized implantable devices and surgical instruments. We aim to provide a systematic review of the current literature discussing clinical applications of three-dimensional printing in colorectal surgery.
METHODS: Full-text studies published in English which described the application of 3D printing in pre-surgical planning, advanced surgical planning, and patient education within the field of colorectal surgery were included. Exclusion criteria were duplicate articles, review papers, studies exclusively dealing with surgical training and/or education, studies which used only virtual models, and studies which described colorectal cancer only as it pertained to other organs.
RESULTS: Eighteen studies were included in this review. There were two randomized controlled trials, one retrospective outcomes study, five case reports/series, one animal model, and nine technical notes/feasibility studies. There were three studies on advanced surgical planning/device manufacturing, six on pre-surgical planning, two on pelvic anatomy modeling, eight on various types of anatomy modeling, and one on patient education.
CONCLUSIONS: While more studies with a higher level of evidence are needed, the findings of this review suggest many promising applications of three-dimensional printing within the field of colorectal surgery with the potential to improve patient outcomes and experiences.

Keywords:  3D model; 3D printing; Anatomy model; Colorectal surgery; Presurgical planning

DOI:  https://doi.org/10.1007/s00384-024-04695-8
Macromol Biosci. 2024 Aug 03. e2400136

Fabrication of GelMA - Agarose Based 3D Bioprinted Photocurable Hydrogel with In Vitro Cytocompatibility and Cells Mirroring Natural Keratocytes for Corneal Stromal Regeneration.

Renuka Vijayaraghavan, Sravanthi Loganathan, Ravi Babu Valapa.

  The complex anatomy of the cornea and the subsequent keratocyte-fibroblast transition have always made corneal stromal regeneration difficult. Recently, 3D printing has received considerable attention in terms of fabrication of scaffolds with precise dimension and pattern. In the current work, 3D printable polymer hydrogels made of GelMA/agarose are formulated and its rheological properties are evaluated. Despite the variation in agarose content, both the hydrogels exhibited G'>G'' modulus. A prototype for 3D stromal model is created using Solid Works software, mimicking the anatomy of an adult cornea. The fabrication of 3D-printed hydrogels is performed using pneumatic extrusion. The FTIR analysis speculated that the hydrogel is well crosslinked and established strong hydrogen bonding with each other, thus contributing to improved thermal and structural stability. The MTT analysis revealed a higher rate of cell proliferation on the hydrogels. The optical analysis carried out on the 14th day of incubation revealed that the hydrogels exhibit transparency matching with natural corneal stromal tissue. Specific protein marker expression confirmed the keratocyte phenotype and showed that the cells do not undergo terminal differentiation into stromal fibroblasts. The findings of this work point to the potential of GelMA/A hydrogels as a novel biomaterial for corneal stromal tissue engineering.

Keywords:  3D printing; GelMA; agarose; corneal stroma; hydrogel; tissue engineering

DOI:  https://doi.org/10.1002/mabi.202400136
Biotechnol J. 2024 Aug;19(8): e2400288

3D-printed titanium scaffolds loaded with gelatin hydrogel containing strontium-doped silver nanoparticles promote osteoblast differentiation and antibacterial activity for bone tissue engineering.

Ramprasad Anushikaa, S Shree Ganesh, Venkadesan Sri Swetha Victoria, Abinaya Shanmugavadivu, Krishnaraj Lavanya, Sundaravadhanan Lekhavadhani, Nagarajan Selvamurugan.

  Bone tissue engineering offers a promising alternative to stimulate the regeneration of damaged tissue, overcoming the limitations of conventional autografts and allografts. Recently, titanium alloy (Ti) implants have garnered significant attention for treating critical-sized bone defects, especially with the advancement of 3D printing technology. Although Ti alloys have impressive versatility, their lack of cellular adhesion, osteogenic and antibacterial properties are significant factors that contribute to their failure. Hence, to overcome these obstacles, this study aimed to incorporate osteoinductive and antibacterial cue-loaded hydrogels into 3D-printed Ti (3D-Ti) scaffolds. 3D-Ti scaffolds were synthesized using the direct metal laser sintering method and loaded with a gelatin (Gel) hydrogel containing strontium-doped silver nanoparticles (Sr-Ag NPs). Compared with Ag NPs, Sr-doped Ag NPs increased the expression of Runx2 mRNA, which is a key bone transcription factor. We subjected the bioactive 3D-hybrid scaffolds (3D-Ti/Gel/Sr-Ag NPs) to physicochemical and material characterization, followed by cytocompatibility and osteogenic evaluation. The microporous and macroporous topographies of the scaffolds with Sr-Ag NPs showed increased Runx2 expression and matrix mineralization, with potent antibacterial properties. Therefore, the 3D-Ti scaffolds incorporated with Sr-Ag NP-loaded Gel hydrogels favored osteoblast differentiation and antibacterial activity, indicating their potential for orthopedic applications.

Keywords:  antibacterial; bone tissue engineering; osseointegration; runx2; titanium alloy

DOI:  https://doi.org/10.1002/biot.202400288
Int J Pharm. 2024 Aug 03. pii: S0378-5173(24)00784-1. [Epub ahead of print]663 124550

Extrusion-based 3D printing for development of complex capsular systems for advanced drug delivery.

Mohammed S Algahtani, Javed Ahmad, Abdul Aleem Mohammed, Mohammad Zaki Ahmad.

  This review explores the feasibility of extrusion-based 3D printing techniques for producing complex dosage forms (such as capsular shells/devices) that provide controlled drug release and targeted delivery. The current discussion explores how extrusion-based 3D printing techniques, particularly Fused Deposition Modelling (FDM) and Pressure-Assisted Modelling (PAM), offer significant advantages in fabricating such complex dosage forms. This technology enables the fabrication of single-, dual-, or multi-compartment capsular systems with customized designs/geometry of the capsular shell to achieve delayed, sustained, or pulsatile drug release. The impact of customized design/geometry on the biopharmaceutical performances of loaded therapeutics is comprehensively discussed. The potential of 3D printing techniques for different specialized drug delivery purposes like gastric floating, implants, suppositories, and printfills are also addressed. This technique has the potential to significantly improve the therapeutic outcomes, and patient adherence to medication regimens, and pave the way for personalized medicine.

Keywords:  3D printing; Capsular shell; Controlled release; FDM; PAM; Personalized medicine; Targeted delivery

DOI:  https://doi.org/10.1016/j.ijpharm.2024.124550
Science. 2024 Aug 09. 385(6709): 604-606

Regenerative cell therapy with 3D bioprinting.

Yu Shrike Zhang, Alireza Dolatshahi-Pirouz, Gorka Orive.

In situ additive biomanufacturing of structures may boost regenerative medicine.

DOI: https://doi.org/10.1126/science.add8593
Drug Deliv Transl Res. 2024 Aug 05.

3D printed microneedles: revamping transdermal drug delivery systems.

Ashlesh Prabhu, Vishal Baliga, Raghavendra Shenoy, Akanksha D Dessai, Usha Y Nayak.

  One of the advancements of the transdermal drug delivery system (TDDS) is the development of microneedles (MNs). These micron-sized needles are used for delivering various types of drugs to address the disadvantage of other transdermal techniques as well as oral drug delivery systems. MNs have high patient acceptance due to self-administration with minimally invasive and pain compared to the parenteral drug delivery. Over the years, various methods have been adopted to evolve the MNs and make them more cost-effective, accurate, and suitable for multiple applications. One such method is the 3D printing of MNs. The development of MN platforms using 3D printing has been made possible by improved features like precision, printing resolution, and the feasibility of using low-cost raw materials. In this review, we have tried to explain various types of MNs, fabrication methods, materials used in the formulation of MNs, and the recent applications that utilize 3D-printed MNs.

Keywords:  3D printing; Fused deposition modelling; Microneedles; Stereolithography; Transdermal drug delivery systems

DOI:  https://doi.org/10.1007/s13346-024-01679-7
Surg Radiol Anat. 2024 Aug 09.

Research and application discussion of cranial bone model preparation method based on three-dimensional reconstruction and 3D printing technology.

Jing Peng, Wenjie Guo, Deqin Yang, Guohui Yang, Yanhong Shu, Ying Li, Libing Rao, Penghui Yu, Li Li.

   PURPOSE: The aim of this study was to find an alternative method to meet traditional human anatomy teaching and clinical needs in order to solve the problem of cranial specimen attrition and specimen resource shortage due to long-term use.
METHODS: We performed a computed tomography (CT) scan of a well-preserved male cranial specimen and used Mimics 19.0 software for 3D reconstruction and cranial block separation. Subsequently, we compared the recognition ability of the processed cranial digital model with that of the 3D body digital model and used 3D printing to create the cranial model and compare it with the physical specimen.
RESULTS: Twenty-two cranial bone block models were obtained, excluding the hyoid bone. Their 3D reconstructed digital models had better bony landmark recognition than the 3D body human digital models, and the differences between the 3D printed models and the physical specimens were minimal. In addition, only one stereolithography (STL) file was required to produce the cranial models, which facilitates repetitive printing at any time.
CONCLUSION: By isolating cranial bone blocks through 3D reconstruction techniques and preparing high-quality cranial models in combination with 3D printing techniques, this study solves the problem of shortage of cranial teaching specimens for the sustainable development of clinical and medical schools.

Keywords:  3D Printing; 3D Reconstruction; Cranial separation; Cranial specimens; Teaching resource

DOI:  https://doi.org/10.1007/s00276-024-03455-1
Methods Mol Biol. 2024 ;2835 269-275

Three-Dimensional Electrically Conductive Scaffolds to Culture Cardiac Progenitor Cells.

Arsalan Ul Haq, Felicia Carotenuto, Fabio De Matteis, Paolo Di Nardo.

  Three-dimensional (3D) scaffolds provide cell support while improving tissue regeneration through amplified cellular responses between implanted materials and native tissues. So far, highly conductive cardiac, nerve, and muscle tissues have been engineered by culturing stem cells on electrically inert scaffolds. These scaffolds, even though suitable, may not be very useful compared to the results shown by cells when cultured on conductive scaffolds. Noticing the mature phenotype the stem cells develop over time when cultured on conductive scaffolds, scientists have been trying to impart conductivity to traditionally nonconductive scaffolds. One way to achieve this goal is to blend conductive polymers (polyaniline, polypyrrole, PEDOT:PSS) with inert biomaterials and produce a 3D scaffold using various fabrication techniques. One such technique is projection micro-stereolithography, which is an additive manufacturing technique. It uses a photosensitive solution blended with conductive polymers and uses visible/UV light to crosslink the solution. 3D scaffolds with complex architectural features down to microscale resolution can be printed with this technique promptly. This chapter reports a protocol to fabricate electrically conductive scaffolds using projection micro-stereolithography.

Keywords:  3D cell culture; Additive manufacturing; Cardiac progenitor cells; Conductive polymers; Scaffolds; Tissue engineering

DOI:  https://doi.org/10.1007/978-1-0716-3995-5_22