bims-biprem Biomed News
on Bioprinting for regenerative medicine
Issue of 2024–07–28
eleven papers selected by
Seerat Maqsood, University of Teramo
  1. Recent advances and applications of artificial intelligence in 3D bioprinting.
  2. Light-based 3D bioprinting technology applied to repair and regeneration of different tissues: A rational proposal for biomedical applications.
  3. Advancements in three-dimensional bioprinting for reproductive medicine: a systematic review.
  4. Recent Advances in Hydrogel-Based 3D Bioprinting and Its Potential Application in the Treatment of Congenital Heart Disease.
  5. Focus on seed cells: stem cells in 3D bioprinting of corneal grafts.
  6. Hydrogel-Based 3D Bioprinting Technology for Articular Cartilage Regenerative Engineering.
  7. Bridging the Gap: Integrating 3D Bioprinting and Microfluidics for Advanced Multi-Organ Models in Biomedical Research.
  8. Advances in tumor microenvironment: Applications and challenges of 3D bioprinting.
  9. 3D-printed microrobots for biomedical applications.
  10. Emerging Biomedical and Clinical Applications of 3D-Printed Poly(Lactic Acid)-Based Devices and Delivery Systems.
  11. Mechanical and in vitro study of 3D printed silk fibroin and bone-based composites biomaterials for bone implant application.
  1. Biophys Rev (Melville). 2024 Sep;5(3): 031301
    Recent advances and applications of artificial intelligence in 3D bioprinting.
    Hongyi Chen, Bin Zhang, Jie Huang.
      3D bioprinting techniques enable the precise deposition of living cells, biomaterials, and biomolecules, emerging as a promising approach for engineering functional tissues and organs. Meanwhile, recent advances in 3D bioprinting enable researchers to build in vitro models with finely controlled and complex micro-architecture for drug screening and disease modeling. Recently, artificial intelligence (AI) has been applied to different stages of 3D bioprinting, including medical image reconstruction, bioink selection, and printing process, with both classical AI and machine learning approaches. The ability of AI to handle complex datasets, make complex computations, learn from past experiences, and optimize processes dynamically makes it an invaluable tool in advancing 3D bioprinting. The review highlights the current integration of AI in 3D bioprinting and discusses future approaches to harness the synergistic capabilities of 3D bioprinting and AI for developing personalized tissues and organs.
    DOI:  https://doi.org/10.1063/5.0190208
  2. Mater Today Bio. 2024 Aug;27 101135
    Light-based 3D bioprinting technology applied to repair and regeneration of different tissues: A rational proposal for biomedical applications.
    Wenzhuo Fang, Zhenwei Yu, Guo Gao, Ming Yang, Xuan Du, Ying Wang, Qiang Fu.
      3D bioprinting technology, a subset of 3D printing technology, is currently witnessing widespread utilization in tissue repair and regeneration endeavors. In particular, light-based 3D bioprinting technology has garnered significant interest and favor. Central to its successful implementation lies the judicious selection of photosensitive polymers. Moreover, by fine-tuning parameters such as light irradiation time, choice of photoinitiators and crosslinkers, and their concentrations, the properties of the scaffolds can be tailored to suit the specific requirements of the targeted tissue repair sites. In this comprehensive review, we provide an overview of commonly utilized bio-inks suitable for light-based 3D bioprinting, delving into the distinctive characteristics of each material. Furthermore, we delineate strategies for bio-ink selection tailored to diverse repair locations, alongside methods for optimizing printing parameters. Ultimately, we present a coherent synthesis aimed at enhancing the practical application of light-based 3D bioprinting technology in tissue engineering, while also addressing current challenges and future prospects.
    Keywords:  Bio-ink 2; Hydrogel 5; Light-based 3D bioprinting 1; Photo-crosslinked polymers 4; Tissue engineering 3
    DOI:  https://doi.org/10.1016/j.mtbio.2024.101135
  3. Reprod Biomed Online. 2024 May 21. pii: S1472-6483(24)00462-0. [Epub ahead of print]49(4): 104273
    Advancements in three-dimensional bioprinting for reproductive medicine: a systematic review.
    Serdar Aydin, Mert Yaşlı, Şule Yildiz, Bulent Urman.
      Reproductive failure due to age, genetics and disease necessitates innovative solutions. While reproductive tissue transplantation has advanced, ongoing research seeks superior approaches. Biomaterials, bioengineering and additive manufacturing, such as three-dimensional (3D) bioprinting, are harnessed to restore reproductive function. 3D bioprinting uses materials, cells and growth factors to mimic natural tissues, proving popular for tissue engineering, notably in complex scaffold creation with cell distribution. The versatility which is brought to reproductive medicine by 3D bioprinting allows more accurate and on-site applicability to various problems that are encountered in the field. However, in the literature, there is a lack of studies encompassing the valuable applications of 3D bioprinting in reproductive medicine. This systematic review aims to improve understanding, and focuses on applications in several branches of reproductive medicine. Advancements span the restoration of ovarian function, endometrial regeneration, vaginal reconstruction, and male germ cell bioengineering. 3D bioprinting holds untapped potential in reproductive medicine.
    Keywords:  3D bioprinting; Endometrium; Ovary; Reproduction; Testis; Vagina
    DOI:  https://doi.org/10.1016/j.rbmo.2024.104273
  4. Biomolecules. 2024 Jul 18. pii: 861. [Epub ahead of print]14(7):
    Recent Advances in Hydrogel-Based 3D Bioprinting and Its Potential Application in the Treatment of Congenital Heart Disease.
    Tasneem Salih, Massimo Caputo, Mohamed T Ghorbel.
      Congenital heart disease (CHD) is the most common birth defect, requiring invasive surgery often before a child's first birthday. Current materials used during CHD surgery lack the ability to grow, remodel, and regenerate. To solve those limitations, 3D bioprinting is an emerging tool with the capability to create tailored constructs based on patients' own imaging data with the ability to grow and remodel once implanted in children with CHD. It has the potential to integrate multiple bioinks with several cell types and biomolecules within 3D-bioprinted constructs that exhibit good structural fidelity, stability, and mechanical integrity. This review gives an overview of CHD and recent advancements in 3D bioprinting technologies with potential use in the treatment of CHD. Moreover, the selection of appropriate biomaterials based on their chemical, physical, and biological properties that are further manipulated to suit their application are also discussed. An introduction to bioink formulations composed of various biomaterials with emphasis on multiple cell types and biomolecules is briefly overviewed. Vasculogenesis and angiogenesis of prefabricated 3D-bioprinted structures and novel 4D printing technology are also summarized. Finally, we discuss several restrictions and our perspective on future directions in 3D bioprinting technologies in the treatment of CHD.
    Keywords:  3D bioprinting technologies; biomaterials; biomolecules; cells; children; congenital heart disease; constructs; grow; hydrogel properties
    DOI:  https://doi.org/10.3390/biom14070861
  5. Front Bioeng Biotechnol. 2024 ;12 1423864
    Focus on seed cells: stem cells in 3D bioprinting of corneal grafts.
    Zi-Jun Xie, Bo-Wei Yuan, Miao-Miao Chi, Jing Hong.
      Corneal opacity is one of the leading causes of severe vision impairment. Corneal transplantation is the dominant therapy for irreversible corneal blindness. However, there is a worldwide shortage of donor grafts and consequently an urgent demand for alternatives. Three-dimensional (3D) bioprinting is an innovative additive manufacturing technology for high-resolution distribution of bioink to construct human tissues. The technology has shown great promise in the field of bone, cartilage and skin tissue construction. 3D bioprinting allows precise structural construction and functional cell printing, which makes it possible to print personalized full-thickness or lamellar corneal layers. Seed cells play an important role in producing corneal biological functions. And stem cells are potential seed cells for corneal tissue construction. In this review, the basic anatomy and physiology of the natural human cornea and the grafts for keratoplasties are introduced. Then, the applications of 3D bioprinting techniques and bioinks for corneal tissue construction and their interaction with seed cells are reviewed, and both the application and promising future of stem cells in corneal tissue engineering is discussed. Finally, the development trends requirements and challenges of using stem cells as seed cells in corneal graft construction are summarized, and future development directions are suggested.
    Keywords:  3D bioprinting; cornea; keratoplasty; regenerative medicine; stem cells
    DOI:  https://doi.org/10.3389/fbioe.2024.1423864
  6. Gels. 2024 Jun 28. pii: 430. [Epub ahead of print]10(7):
    Hydrogel-Based 3D Bioprinting Technology for Articular Cartilage Regenerative Engineering.
    Hongji Zhang, Zheyuan Zhou, Fengjie Zhang, Chao Wan.
      Articular cartilage is an avascular tissue with very limited capacity of self-regeneration. Trauma or injury-related defects, inflammation, or aging in articular cartilage can induce progressive degenerative joint diseases such as osteoarthritis. There are significant clinical demands for the development of effective therapeutic approaches to promote articular cartilage repair or regeneration. The current treatment modalities used for the repair of cartilage lesions mainly include cell-based therapy, small molecules, surgical approaches, and tissue engineering. However, these approaches remain unsatisfactory. With the advent of three-dimensional (3D) bioprinting technology, tissue engineering provides an opportunity to repair articular cartilage defects or degeneration through the construction of organized, living structures composed of biomaterials, chondrogenic cells, and bioactive factors. The bioprinted cartilage-like structures can mimic native articular cartilage, as opposed to traditional approaches, by allowing excellent control of chondrogenic cell distribution and the modulation of biomechanical and biochemical properties with high precision. This review focuses on various hydrogels, including natural and synthetic hydrogels, and their current developments as bioinks in 3D bioprinting for cartilage tissue engineering. In addition, the challenges and prospects of these hydrogels in cartilage tissue engineering applications are also discussed.
    Keywords:  bioink; biomaterials; cartilage; hydrogel; three-dimensional bioprinting; tissue engineering
    DOI:  https://doi.org/10.3390/gels10070430
  7. Bioengineering (Basel). 2024 Jun 28. pii: 664. [Epub ahead of print]11(7):
    Bridging the Gap: Integrating 3D Bioprinting and Microfluidics for Advanced Multi-Organ Models in Biomedical Research.
    Marco De Spirito, Valentina Palmieri, Giordano Perini, Massimiliano Papi.
      Recent advancements in 3D bioprinting and microfluidic lab-on-chip systems offer promising solutions to the limitations of traditional animal models in biomedical research. Three-dimensional bioprinting enables the creation of complex, patient-specific tissue models that mimic human physiology more accurately than animal models. These 3D bioprinted tissues, when integrated with microfluidic systems, can replicate the dynamic environment of the human body, allowing for the development of multi-organ models. This integration facilitates more precise drug screening and personalized therapy development by simulating interactions between different organ systems. Such innovations not only improve predictive accuracy but also address ethical concerns associated with animal testing, aligning with the three Rs principle. Future directions include enhancing bioprinting resolution, developing advanced bioinks, and incorporating AI for optimized system design. These technologies hold the potential to revolutionize drug development, regenerative medicine, and disease modeling, leading to more effective, personalized, and humane treatments.
    Keywords:  3D printing; lab-on-chip; microfluidics; multi-organ
    DOI:  https://doi.org/10.3390/bioengineering11070664
  8. Biochem Biophys Res Commun. 2024 Jul 08. pii: S0006-291X(24)00875-1. [Epub ahead of print]730 150339
    Advances in tumor microenvironment: Applications and challenges of 3D bioprinting.
    Yingying Dong, Xue Zhou, Yunyi Ding, Yichen Luo, Hong Zhao.
      The tumor microenvironment (TME) assumes a pivotal role in the treatment of oncological diseases, given its intricate interplay of diverse cellular components and extracellular matrices. This dynamic ecosystem poses a serious challenge to traditional research methods in many ways, such as high research costs, inefficient translation, poor reproducibility, and low modeling success rates. These challenges require the search for more suitable research methods to accurately model the TME, and the emergence of 3D bioprinting technology is transformative and an important complement to these traditional methods to precisely control the distribution of cells, biomolecules, and matrix scaffolds within the TME. Leveraging digital design, the technology enables personalized studies with high precision, providing essential experimental flexibility. Serving as a critical bridge between in vitro and in vivo studies, 3D bioprinting facilitates the realistic 3D culturing of cancer cells. This comprehensive article delves into cutting-edge developments in 3D bioprinting, encompassing diverse methodologies, biomaterial choices, and various 3D tumor models. Exploration of current challenges, including limited biomaterial options, printing accuracy constraints, low reproducibility, and ethical considerations, contributes to a nuanced understanding. Despite these challenges, the technology holds immense potential for simulating tumor tissues, propelling personalized medicine, and constructing high-resolution organ models, marking a transformative trajectory in oncological research.
    Keywords:  3D bioprinting technology; Cancer; The tumor microenvironment; Tumor models
    DOI:  https://doi.org/10.1016/j.bbrc.2024.150339
  9. Biomater Sci. 2024 Jul 23.
    3D-printed microrobots for biomedical applications.
    Kun Wei, Chenlong Tang, Hui Ma, Xingmiao Fang, Runhuai Yang.
      Microrobots, which can perform tasks in difficult-to-reach parts of the human body under their own or external power supply, are potential tools for biomedical applications, such as drug delivery, microsurgery, imaging and monitoring, tissue engineering, and sensors and actuators. Compared with traditional fabrication methods for microrobots, recent improvements in 3D printers enable them to print high-precision microrobots, breaking through the limitations of traditional micromanufacturing technologies that require high skills for operators and greatly shortening the design-to-production cycle. Here, this review first introduces typical 3D printing technologies used in microrobot manufacturing. Then, the structures of microrobots with different functions and application scenarios are discussed. Next, we summarize the materials (body materials, propulsion materials and intelligent materials) used in 3D microrobot manufacturing to complete body construction and realize biomedical applications (e.g., drug delivery, imaging and monitoring). Finally, the challenges and future prospects of 3D printed microrobots in biomedical applications are discussed in terms of materials, manufacturing and advancement.
    DOI:  https://doi.org/10.1039/d4bm00674g
  10. Bioengineering (Basel). 2024 Jul 11. pii: 705. [Epub ahead of print]11(7):
    Emerging Biomedical and Clinical Applications of 3D-Printed Poly(Lactic Acid)-Based Devices and Delivery Systems.
    Allan John R Barcena, Prashanth Ravi, Suprateek Kundu, Karthik Tappa.
      Poly(lactic acid) (PLA) is widely used in the field of medicine due to its biocompatibility, versatility, and cost-effectiveness. Three-dimensional (3D) printing or the systematic deposition of PLA in layers has enabled the fabrication of customized scaffolds for various biomedical and clinical applications. In tissue engineering and regenerative medicine, 3D-printed PLA has been mostly used to generate bone tissue scaffolds, typically in combination with different polymers and ceramics. PLA's versatility has also allowed the development of drug-eluting constructs for the controlled release of various agents, such as antibiotics, antivirals, anti-hypertensives, chemotherapeutics, hormones, and vitamins. Additionally, 3D-printed PLA has recently been used to develop diagnostic electrodes, prostheses, orthoses, surgical instruments, and radiotherapy devices. PLA has provided a cost-effective, accessible, and safer means of improving patient care through surgical and dosimetry guides, as well as enhancing medical education through training models and simulators. Overall, the widespread use of 3D-printed PLA in biomedical and clinical settings is expected to persistently stimulate biomedical innovation and revolutionize patient care and healthcare delivery.
    Keywords:  3D printing; drug delivery; medical device; polylactic acid; radiotherapy; regenerative medicine; surgery; tissue engineering
    DOI:  https://doi.org/10.3390/bioengineering11070705
  11. Proc Inst Mech Eng H. 2024 Jul 24. 9544119241259071
    Mechanical and in vitro study of 3D printed silk fibroin and bone-based composites biomaterials for bone implant application.
    Ali Imran Ansari, Nazir Ahmad Sheikh, Navin Kumar.
      When treating orthopaedic damage or illness and accidental fracture, bone grafting remains the gold standard of treatment. In cases where this approach doesn't seem achievable, bone tissue engineering can offer scaffolding as a substitute. Defective and fractured bone tissue is extracted and substituted with porous scaffold structures to aid in the process of tissue regeneration. 3D bioprinting has demonstrated enormous promise in recent years for producing scaffold structures with the necessary capabilities. In order to create composite biomaterial inks for 3D bioprinting, three different materials were combined such as silk fibroin, bone particles, and synthetic biopolymer poly (ε-caprolactone) (PCL). These biomaterials were used to fabricate the two composites scaffolds such as: silk fibroin + bovine bone (SFB) and silk fibroin + bovine bone + Polycaprolactone (SFBP). The biomechanical, structural, and biological elements of the manufactured composite scaffolds were characterized in order to determine their suitability as a possible biomaterial for the production of bone tissue. The in vitro bioactivity of the two composite scaffolds was assessed in the simulated body fluids, and the swelling and degradation characteristics of the two developed scaffolds were analyzed separately over time. The results showed that the mechanical durability of the composite scaffolds was enhanced by the bovine bone particles, up to a specific concentration in the silk fibroin matrix. Furthermore, the incorporation of bone particles improved the bioactive composite scaffolds' capacity to generate hydroxyapatite in vitro. The combined findings show that the two 3D printed bio-composites scaffolds have the required mechanical strength and may be applied to regeneration of bone tissue and restoration, since they resemble the characteristics of native bone.
    Keywords:  3D bioprinting; 3D printed scaffolds; Silk fibroin based composites ink; bovine bone particles based composites ink
    DOI:  https://doi.org/10.1177/09544119241259071
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