bims-biprem Biomed News
on Bioprinting for regenerative medicine
Issue of 2024–07–14
nine papers selected by
Seerat Maqsood, University of Teramo
  1. A Comprehensive Literature Review on Advancements and Challenges in 3D Bioprinting of Human Organs: Ear, Skin, and Bone.
  2. Translational Aspects of 3D and 4D Printing and Bioprinting.
  3. Biofabrication and Monitoring of a 3D Printed Skin Model for Melanoma.
  4. 3D Bioprinting with Visible Light Cross-Linkable Mucin-Hyaluronic Acid Composite Bioink for Lung Tissue Engineering.
  5. Engineering biomaterials by inkjet printing of hydrogels with functional particulates.
  6. Advanced biomedical and electronic dual-function skin patch created through microfluidic-regulated 3D bioprinting.
  7. 3D-Bioprinted Skin Tissues for Improving Wound Healing: Current Status and Perspective.
  8. The comprehensive review on 3D printing- pharmaceutical drug delivery and personalized food and nutrition.
  9. Fullerol-reinforced antioxidantive 3D-printed bredigite scaffold for accelerating bone healing.
  1. Ann Biomed Eng. 2024 Jul 08.
    A Comprehensive Literature Review on Advancements and Challenges in 3D Bioprinting of Human Organs: Ear, Skin, and Bone.
    Aishwarya Varpe, Marwana Sayed, Nikhil S Mane.
      The field of 3D bioprinting is rapidly emerging within the realm of regenerative medicine, offering significant potential in dealing with the issue of organ shortages. Despite being in its early stages, it has the potential to replicate tissue structures accurately, providing new potential solutions for reconstructive surgery. This review explores the diverse applications of 3D bioprinting in regenerative medicine, pharmaceuticals, and the food industry, specifically focusing on ear, skin, and bone tissues due to their unique challenges and implications in the field. Significant progress has been made in cartilage and bone scaffold fabrication in ear reconstruction, yet challenges in functional maturation persist. Recent advancements highlight the potential for patient-specific ear substitutes, emphasizing the need for extensive clinical trials. In skin regeneration, 3D bioprinting addresses limitations in existing models, offering opportunities for improved wound healing and realistic skin models. While challenges exist, progress in biomaterials and in-situ bioprinting holds promise. In bone regeneration, 3D bioprinting presents personalized solutions for defects, but scaffold design refinement and addressing regulatory and ethical considerations are crucial. The transformative potential of 3D bioprinting in the field of medicine holds the promise of redefining therapeutic approaches and delivering personalized treatments and functional tissues. Interdisciplinary collaboration is essential for fully realizing the capabilities of 3D bioprinting. This review provides a detailed analysis of current methodologies, challenges, and prospects in 3D bioprinting for ear, skin, and bone tissue regeneration.
    Keywords:  3D bioprinting; Biomaterials; Bone; Ear; Reconstructive surgery; Regenerative medicine; Skin
    DOI:  https://doi.org/10.1007/s10439-024-03580-3
  2. Adv Healthc Mater. 2024 Jul 09. e2400463
    Translational Aspects of 3D and 4D Printing and Bioprinting.
    Scott Taylor, Eva Mueller, Lamont R Jones, Ashley V Makela, Nureddin Ashammakhi.
      Three-dimensional (3D) printed medical devices include orthopedic and craniofacial implants, surgical tools, and external prosthetics that have been directly used in patients. While the advances of additive manufacturing techniques in the production of medical devices have been on the rise, clinical translation of living cellular constructs face significant limitations in terms of regulatory affairs, process technology, and materials development. In this perspective, the current status-quo of 3D and four-dimensional (4D) (bio)printing is summarized, current advancements are discussed and the challenges that need to be addressed for improved industrial translation and clinical applications of bioprinting are highlighted. It is focused on a multidisciplinary approach in discussing the key translational considerations, from the perspective of industry, regulatory bodies, funding strategies, and future directions.
    Keywords:  3D printing; 4D printing; regulatory approval; scale‐up; translational research
    DOI:  https://doi.org/10.1002/adhm.202400463
  3. Adv Healthc Mater. 2024 Jul 11. e2401136
    Biofabrication and Monitoring of a 3D Printed Skin Model for Melanoma.
    Paula Vázquez-Aristizabal, Malou Henriksen-Lacey, Clara García-Astrain, Dorleta Jimenez de Aberasturi, Judith Langer, Claudia Epelde, Lucio Litti, Luis M Liz-Marzán, Ander Izeta.
      There is an unmet need for in vitro cancer models that emulate the complexity of human tissues. 3D-printed solid tumor micromodels based on decellularized extracellular matrices (dECMs) recreate the biomolecule-rich matrix of native tissue. Herein a 3D in vitro metastatic melanoma model that is amenable for drug screening purposes and recapitulates features of both the tumor and the skin microenvironment is described. Epidermal, basement membrane, and dermal biocompatible inks are prepared by means of combined chemical, mechanical, and enzymatic processes. Bioink printability is confirmed by rheological assessment and bioprinting, and bioinks are subsequently combined with melanoma cells and dermal fibroblasts to build complex 3D melanoma models. Cells are tracked by confocal microscopy and surface-enhanced Raman spectroscopy (SERS) mapping. Printed dECMs and cell tracking allow modeling of the initial steps of metastatic disease, and may be used to better understand melanoma cell behavior and response to drugs.
    Keywords:  3D bioprinting; SERS; dECM; melanoma; metastasis
    DOI:  https://doi.org/10.1002/adhm.202401136
  4. ACS Appl Bio Mater. 2024 Jul 12.
    3D Bioprinting with Visible Light Cross-Linkable Mucin-Hyaluronic Acid Composite Bioink for Lung Tissue Engineering.
    Sruthi C Sasikumar, Upashi Goswami, Ashok M Raichur.
      3D printing can revolutionize personalized medicine by allowing cost-effective, customized tissue-engineering constructs. However, the limited availability and diversity of biopolymeric hydrogels restrict the variety and applications of bioinks. In this study, we introduce a composite bioink for 3D bioprinting, combining a photo-cross-linkable derivative of Mucin (Mu) called Methacrylated Mucin (MuMA) and Hyaluronic acid (HA). The less explored Mucin is responsible for the hydrogel nature of mucus and holds the potential to be used as a bioink material because of its plethora of features. HA, a crucial extracellular matrix component, is mucoadhesive and enhances ink viscosity and printability. Photo-cross-linking with 405 nm light stabilizes the printed scaffolds without damaging cells. Rheological tests reveal shear-thinning behavior, aiding cell protection during printing and improved MuMA bioink viscosity by adding HA. The printed structures exhibited porous behavior conducive to nutrient transport and cell migration. After 4 weeks in phosphate-buffered saline, the scaffolds retain 70% of their mass, highlighting stability. Biocompatibility tests with lung epithelial cells (L-132) confirm cell attachment and growth, suggesting suitability for lung tissue engineering. It is envisioned that the versatility of bioink could lead to significant advancements in lung tissue engineering and various other biomedical applications.
    Keywords:  3D bioprinting; bioink; hyaluronic acid; lung tissue engineering; mucin; scaffolds
    DOI:  https://doi.org/10.1021/acsabm.4c00579
  5. Med X. 2024 ;2(1): 9
    Engineering biomaterials by inkjet printing of hydrogels with functional particulates.
    Cih Cheng, Eric J Williamson, George T-C Chiu, Bumsoo Han.
      Hydrogels with particulates, including proteins, drugs, nanoparticles, and cells, enable the development of new and innovative biomaterials. Precise control of the spatial distribution of these particulates is crucial to produce advanced biomaterials. Thus, there is a high demand for manufacturing methods for particle-laden hydrogels. In this context, 3D printing of hydrogels is emerging as a promising method to create numerous innovative biomaterials. Among the 3D printing methods, inkjet printing, so-called drop-on-demand (DOD) printing, stands out for its ability to construct biomaterials with superior spatial resolutions. However, its printing processes are still designed by trial and error due to a limited understanding of the ink behavior during the printing processes. This review discusses the current understanding of transport processes and hydrogel behaviors during inkjet printing for particulate-laden hydrogels. Specifically, we review the transport processes of water and particulates within hydrogel during ink formulation, jetting, and curing. Additionally, we examine current inkjet printing applications in fabricating engineered tissues, drug delivery devices, and advanced bioelectronics components. Finally, the challenges and opportunities for next-generation inkjet printing are also discussed.
    Graphical Abstract:
    Keywords:  Bioelectronics; Drop-on-demand printing; Drug delivery device; Particle transport; Tissue engineering; Water-matrix interaction
    DOI:  https://doi.org/10.1007/s44258-024-00024-4
  6. Bioact Mater. 2024 Oct;40 261-274
    Advanced biomedical and electronic dual-function skin patch created through microfluidic-regulated 3D bioprinting.
    Ting Dong, Jie Hu, Yue Dong, Ziyi Yu, Chang Liu, Gefei Wang, Su Chen.
      Artificial skin involves multidisciplinary efforts, including materials science, biology, medicine, and tissue engineering. Recent studies have aimed at creating skins that are multifunctional, intelligent, and capable of regenerating tissue. In this work, we present a specialized 3D printing ink composed of polyurethane and bioactive glass (PU-BG) and prepare dual-function skin patch by microfluidic-regulated 3D bioprinting (MRBP) technique. The MRBP endows the skin patch with a highly controlled microstructure and superior strength. Besides, an asymmetric tri-layer is further constructed, which promotes cell attachment and growth through a dual transport mechanism based on hydrogen bonds and gradient structure from hydrophilic to superhydrophilic. More importantly, by combining the features of biomedical skin with electronic skin (e-skin), we achieved a biomedical and electronic dual-function skin patch. In vivo experiments have shown that this skin patch can enhance hemostasis, resist bacterial growth, stimulate the regeneration of blood vessels, and accelerate the healing process. Meanwhile, it also mimics the sensory functions of natural skin to realize signal detection, where the sensitivity reached up to 5.87 kPa-1, as well as cyclic stability (over 500 cycles), a wide detection range of 0-150 kPa, high pressure resolution of 0.1 % under the pressure of 100 kPa. This work offers a versatile and effective method for creating dual-function skin patches and provide new insights into wound healing and tissue repair, which have significant implications for clinical applications.
    Keywords:  3D bioprinting; Pressure sensor; Skin patches; Wound healing
    DOI:  https://doi.org/10.1016/j.bioactmat.2024.06.015
  7. Adv Exp Med Biol. 2024 Jul 10.
    3D-Bioprinted Skin Tissues for Improving Wound Healing: Current Status and Perspective.
    Nikita Gopakumar, Abdulla M Ali, Sumayah Oudda, Amarnath Singam, Seungman Park.
      Advancements in tissue engineering enable the fabrication of complex and functional tissues or organs. In particular, bioprinting enables controlled and accurate deposition of cells, biomaterials, and growth factors to create complex 3D skin constructs specific to a particular individual. Despite these advancements, challenges such as vascularization, long-term stability, and regulatory considerations hinder the clinical translation of bioprinted skin constructs. This chapter focuses on such approaches using advanced biomaterials and bioprinting techniques to overcome the current barriers in wound-healing studies. Moreover, it addresses current obstacles in wound-healing studies, highlighting the need for continued research and innovation to overcome these barriers and facilitate the practical utilization of bioprinted skin constructs in clinical settings.
    Keywords:  Bioprinting; Dermis; Epidermis; Hypodermis; Skin tissue; Wound healing
    DOI:  https://doi.org/10.1007/5584_2024_817
  8. Food Chem. 2024 Jul 05. pii: S0308-8146(24)01998-8. [Epub ahead of print]459 140348
    The comprehensive review on 3D printing- pharmaceutical drug delivery and personalized food and nutrition.
    Meenakshi Murugan, Selva Kumar Ramasamy, Geetha Venkatesan, Jintae Lee, Selvaraj Barathi, Sabariswaran Kandasamy, Prakash Kumar Sarangi.
      Three-dimensional printing is one of the emerging technologies that is gaining interest from the pharmaceutical industry as it provides an opportunity to customize drugs according to each patient's needs. Combining different active pharmaceutical ingredients, using different geometries, and providing sustained release enhances the effectiveness of medicine. One of the most innovative uses of 3D printing is producing fabrics, medical devices, medical implants, orthoses, and prostheses. This review summarizes the various 3D printing techniques such as stereolithography, inkjet printing, thermal inkjet printing, fused deposition modelling, extrusion printing, semi-solid extrusion printing, selective laser sintering, and hot-melt extrusion. Also, discusses the drug relies profile and its mechanisms, characteristics, and applications of the most common types of 3D printed API formulations and its recent development. Here, Authors also, summarizes the central flow of 3D food printing process and knowledge extension toward personalized nutrition.
    Keywords:  3D printing; Drug delivery; FDM; Pharma foods; Tablet-in-device
    DOI:  https://doi.org/10.1016/j.foodchem.2024.140348
  9. Mater Today Bio. 2024 Aug;27 101120
    Fullerol-reinforced antioxidantive 3D-printed bredigite scaffold for accelerating bone healing.
    Jielai Yang, Zihang Zhan, Xingchen Li, Mu Hu, Yuan Zhu, Yunchao Xiao, Xiangyang Xu.
      Reactive oxygen species play a vital role in tissue repair, and nonequilibrium of redox homeostasis around bone defect can compromise osteogenesis. However, insufficient antioxidant capacity and weak osteogenic performance remain major obstacles for bone scaffold materials. Herein, integrating the mussel-inspired polydopamine (PDA) coating and 3D printing technologies, we utilized the merits of both osteogenic bredigite and antioxidative fullerol to construct 3D-printed porous, biodegradable acid-buffering, reactive oxygen species (ROS) -scavenging and robust osteogenic bio-scaffold (denoted "FPBS") for in situ bone defect restoration under oxidative stress microenvironment. Initially, fullerol nanoparticles were attached to the surface of the bredigite scaffold via covalently inter-crosslinking with PDA. Upon injury, extracellular ROS capturing triggered the oxidative degradation of PDA, releasing fullerol nanoparticles to enter into cells for further intracellular ROS scavenging. In vitro, FPBS had good biocompatibility and excellent antioxidative capability. Furthermore, FPBS promoted the osteogenesis of stem cells with significant elevation of osteogenic markers. Finally, in vivo implantation of FPBS remarkably enhanced new bone formation in a rat critical calvarial defect model. Overall, with amelioration of the ROS microenvironment of injured tissue and enhancement of osteogenic differentiation of stem cells simultaneously, FPBS may hold great potential towards bone defect repair.
    Keywords:  3D-printing; Bone regeneration; Bredigite scaffold; Fullerol nanoparticle; Reactive oxygen species
    DOI:  https://doi.org/10.1016/j.mtbio.2024.101120
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