bims-resufa Biomed News
on Respiratory supercomplex factors
Issue of 2019‒05‒26
one paper selected by
Vera Strogolova
Marquette University


  1. Proc Natl Acad Sci U S A. 2019 May 20. pii: 201900890. [Epub ahead of print]
    Xu Y, Anjaneyulu M, Donelian A, Yu W, Greenberg ML, Ren M, Owusu-Ansah E, Schlame M.
      Cardiolipin (CL) is a mitochondrial phospholipid with a very specific and functionally important fatty acid composition, generated by tafazzin. However, in vitro tafazzin catalyzes a promiscuous acyl exchange that acquires specificity only in response to perturbations of the physical state of lipids. To identify the process that imposes acyl specificity onto CL remodeling in vivo, we analyzed a series of deletions and knockdowns in Saccharomyces cerevisiae and Drosophila melanogaster, including carriers, membrane homeostasis proteins, fission-fusion proteins, cristae-shape controlling and MICOS proteins, and the complexes I-V. Among those, only the complexes of oxidative phosphorylation (OXPHOS) affected the CL composition. Rather than any specific complex, it was the global impairment of the OXPHOS system that altered CL and at the same time shortened its half-life. The knockdown of OXPHOS expression had the same effect on CL as the knockdown of tafazzin in Drosophila flight muscles, including a change in CL composition and the accumulation of monolyso-CL. Thus, the assembly of OXPHOS complexes induces CL remodeling, which, in turn, leads to CL stabilization. We hypothesize that protein crowding in the OXPHOS system imposes packing stress on the lipid bilayer, which is relieved by CL remodeling to form tightly packed lipid-protein complexes.
    Keywords:  cardiolipin; lipids; membrane; mitochondria; respiration
    DOI:  https://doi.org/10.1073/pnas.1900890116