bims-mecosi Biomed News
on Membrane contact sites
Issue of 2021‒08‒15
one paper selected by
Verena Kohler
Stockholm University


  1. J Cell Sci. 2022 Mar 01. pii: jcs258819. [Epub ahead of print]135(5):
      Lipid droplets (LDs) are globular intracellular structures dedicated to the storage of neutral lipids. They are closely associated with the endoplasmic reticulum (ER) and are delineated by a monolayer of phospholipids that is continuous with the cytoplasmic leaflet of the ER membrane. LDs contain a specific set of proteins, but how these proteins are targeted to the LD surface is not fully understood. Here, we devised a yeast mating-based microscopic readout to monitor the transfer of LD proteins upon zygote formation. The results of this analysis indicate that ER fusion between mating partners is required for transfer of LD proteins and that this transfer is continuous, bidirectional and affects most LDs simultaneously. These observations suggest that LDs do not fuse upon mating of yeast cells, but that they form a network that is interconnected through the ER membrane. Consistent with this, ER-localized LD proteins rapidly move onto LDs of a mating partner and this protein transfer is affected by seipin, a protein important for proper LD biogenesis and the functional connection of LDs with the ER membrane.
    Keywords:   Saccharomyces cerevisiae ; Endoplasmic reticulum; Lipid droplets; Mating; Membrane fusion; Protein targeting; Seipin; Steryl esters; Triacylglycerol
    DOI:  https://doi.org/10.1242/jcs.258819